The present invention relates to a method for production of an improved sensor surface for an SPR instrument, comprising forming a self assembled monolayer (SAM) on a surface and attaching ligands and protein resistant groups, preferably polyethylene glycol (PEG), directly to functional groups on said surface. The invention also relates to a sensor surface produced by these methods use thereof in SPR (surface plasmon resonance) assays or interactions.

The present disclosure provides a method for determining of aggregates comprising one or more macromolecules, in a first sample potentially comprising aggregates of the macromolecule(s), comprising the steps of (a) contacting a first sample with a sensing surface of an interaction analysis sensor, said sensing surface having immobilised thereon a ligand comprising a hydrophobic group, which is capable of increased binding interaction with aggregates of macromolecule(s) compared to non-aggregated macromolecule(s); (b) determining at least one parameter for the interaction of the first sample with the sensing surface; (c) Performing at least one of steps (i) and (ii): (i) Comparing the at least one parameter determined in step (b) with the corresponding parameter(s) determined for at least one additional sample potentially comprising aggregates of the macromolecule(s); (ii) determining at least one parameter related to aggregate(s) of the macromolecule(s); and (d) determining the presence, fraction, concentration, and/or amount of macromolecule(s) in the form of aggregate(s) in the first sample. The present disclosure also relates to uses of said method and an interaction analysis sensor for use in said method, as well as an interaction analysis sensor and a method for determining of the stability of a macromolecule.

Disclosed herein is a biomolecular detection device (1) for analyzing a cell, vesicle or a cellular or vesicular component, comprising an evanescent illuminator with an optical coupling unit configured for generating an evanescent field from coherent light (L) with a predefined wavelength on a first surface of the evanescent illuminator. The first surface of evanescent illuminator comprises a template nanopattern (5), containing a coherent arrangement of a plurality of predetermined lines along which membrane recognition elements for a binder structure (82) of a transmembrane protein (81), preferably a laterally diffusible transmembrane protein, of the cell, vesicle or the cellular or vesicular component (8) are arranged. The membrane recognition elements (53) are configured to bind the binder structure (82) of the transmembrane protein (81) for forming a transmembrane nanopattern within the cell, vesicle or the cellular or vesicular component (8) based on the template nanopattern (5) of the evanescent illuminator, such that light of the evanescent field is scattered by the cell, vesicle or the cellular or vesicular component (8) bound to the membrane recognition elements (53). The predetermined lines are arranged such that light scattered by the cell, vesicle or cellular or vesicular components (8) bound to the membrane recognition elements (53) constructively interferes at a predefined detection site (7) with a difference in optical path length that is an integer multiple of the predefined wavelength of the coherent light (L).

A semiconductor device according to the present invention includes a substrate, a plurality of electrodes on the substrate, an insulator provided with one or a plurality of openings exposing at least one electrode among the plurality of electrodes on the substrate, the insulator covering at least a portion of the plurality of electrodes, and a semiconductor sheet on the insulator and one or a plurality of exposed portions exposed from the one or the plurality of openings on the substrate.

Systems, methods, and apparatus are disclosed for determining quantitative hormone and chemical analyte results from qualitative test results. An image is taken of an ovulation test device. The image is analyzed to identify a color intensity ratio (T/C ratio) between a color density of a test-line to a color density of a control-line. Additionally, a quantitative substance level may be determined using the T/C ratio, by identifying the type of test device and referencing a data structure that relates quantitative substance levels to T/C ratios for the identified type of test device.

Disclosed herein is a method of diagnosing a malignant glioblastoma. The method can comprise of isolating glioma-derived exosomes from a bodily fluid of the subject, and characterizing the amount of Cluster of Differentiation (CD44) and Cluster of Differentiation 133 (CD133) present in the glioma-derived exosomes. This method allows for the diagnosis of a malignant glioblastoma using CD44 and CD133 levels in EGFRviii specific immunocaptured exosomes from bodily fluids, which has previously not been recognized as providing an indication of a glioblastoma.

The present invention relates to a method for virus assay. More closely the invention relates a method for total quantification of adenovirus in a sample as well as total and functional (active) adenovirus in a sample. The method for determining adenovirus concentration in a sample comprises subjecting said sample to SPR (surface plasmon resonance) assay with immobilized FX (Factor X) and/or immobilized CAR (coxsackievirus and adenovirus receptor) on a sensor surface, wherein the adenovirus concentration is determined from sample binding to immobilized FX and/or immobilized CAR. CAR can be replaced by an ligand binding to adenovirus fiber, such as an anti-adenovirus fiber antibody. FX can be replaced by a ligand binding to adenovirus hexon, such as an anti-adenovirus hexon antibody. The method can be used for quality control in an adenovirus purification process, for example for gene therapy.

The present invention provides a sensing device for detecting an analyte containing a non-metallic element such as F. A working sensor has a 3D array of voids each having a void internal wall. The void internal walls have cavities each having a cavity internal wall made from a material containing the non-metallic element. A binding of the analytes to the cavities induces a detectable variation of the optical property of the 3D array of voids. The invention exhibits numerous technical merits such as high sensitivity, high specificity, fast detection, ease of operation, low power consumption, zero chemical release, and low operation cost, among others.

The present disclosure relates generally to a sensor chip and methods for the detection of an analyte. In particular, the disclosure relates to a sensor chip for detecting an analyte in a subject suffering from a neurodegenerative disease. The sensor chip comprises a conductive layer on a membrane support layer, wherein a plurality of apertures extend through the conductive layer and the membrane support layer and are arranged such that illumination of the conductive layer and/or the membrane support layer produces a surface plasmon resonance.

The present disclosure describes methods of detecting viral biomolecules such as viruses through frequency response. A method (200) of detecting a vims includes exposing (210) a sensor surface to a fluid sample containing a suspected virus. The sensor surface can be a surface of a resonator having a clean resonant frequency from about 1 MHz to about 1 GHz. The surface can be modified with molecular recognition groups selective for binding to the viral biomolecule. A resonant frequency of the resonator can be measured (220) after exposing the sensor surface to the fluid sample. The measured resonant frequency can be compared (230) with a clean resonant frequency indicating the presence of the viral biomolecule bound to the molecular recognition groups and then outputted (240) as a detection signal.