Hybrid microparticle having a polymer core and a shell which surrounds the polymer core at least in sections and which has a silicon dioxide layer; characterized by an RF value, the RF value being defined as the ratio of an external surface area amenable to the adsorption of nitrogen to a surface area which is computable from an arithmetic mean diameter of the hybrid microparticle considered as an ideal sphere, where the shell has a structure selected from: closed and smooth, with the shell having an RF value of between 1 and 1.5; closed and hillocky, with the shell having an RF value of between 1.51 and 3; or open, with the shell having an RF value of greater than 3.01.

Particle-based detection of analytes including, for example, systems, kits, and methods for growth, isolation, and/or monitoring of analytes are generally disclosed. In some embodiments, the systems and methods described herein are generally directed to the capture and/or concentrating of a target species (e.g., analyte) to be detected and/or monitored. In some embodiments, the materials, systems, and methods described herein may be used to create luminescent signals in response to the presence of selected analytes such as bacteria, viruses, and parasites. In some cases, the target analyte is a pathogenic bacteria, a pathogenic virus, a pathogenic parasite, or toxin.

Particle-based detection of analytes including, for example, systems, kits, and methods for growth, isolation, and/or monitoring of analytes are generally disclosed. In some embodiments, the systems and methods described herein are generally directed to the capture and/or concentrating of a target species (e.g., analyte) to be detected and/or monitored. In some embodiments, the materials, systems, and methods described herein may be used to create luminescent signals in response to the presence of selected analytes such as bacteria, viruses, and parasites. In some cases, the target analyte is a pathogenic bacteria, a pathogenic virus, a pathogenic parasite, or toxin.

An object of the present invention is to provide a reagent kit, a measurement kit, and a measurement method for immunologically measuring serum amyloid A with high accuracy and high sensitivity without using an alcohol designated as a hazardous material on the fire protection law. According to the present invention, provided is a reagent kit for measuring serum amyloid A, including first particles having a label and modified with a first binding substance having a property of specifically binding to serum amyloid A, and at least one nonionic surfactant having a molecular weight of 1000 or less.

A poly(methyl methacrylate) (PMMA) membrane having a highly porous, reticulated, 3-D structure suitable for lateral flow diagnostic applications is described. Also described is a method for producing a poly(methyl methacrylate) (PMMA) membrane that comprises the steps of mixing a suitable amount of PMMA, a solvent and a optionally one of either a co-solvent or a non-solvent to produce a solution, casting a thin film of the solution onto a support, and removal of the solvent from the solution to produce the PMMA membrane. A lateral flow diagnostic device comprising a highly porous PMMA membrane as a reaction membrane is also described.

A poly(methyl methacrylate) (PMMA) membrane having a highly porous, reticulated, 3-D structure suitable for lateral flow diagnostic applications is described. Also described is a method for producing a poly(methyl methacrylate) (PMMA) membrane that comprises the steps of mixing a suitable amount of PMMA, a solvent and a optionally one of either a co-solvent or a non-solvent to produce a solution, casting a thin film of the solution onto a support, and removal of the solvent from the solution to produce the PMMA membrane. A lateral flow diagnostic device comprising a highly porous PMMA membrane as a reaction membrane is also described.

Provided are methods for detecting a target molecule or particle suspected to be present in a sample, comprising (a) contacting the sample with (i) a fusion molecule comprising a ligand capable of binding to the target molecule or particle and a binding domain, and (ii) a polymer scaffold comprising at least one binding motif to which the binding domain is capable of binding, under conditions that allow the target molecule or particle to bind to the ligand and the binding domain to bind to the binding motif; (b) loading the polymer into a device comprising a pore that separates an interior space of the device into two volumes, and configuring the device to pass the polymer through the pore from one volume to the other volume, wherein the device further comprises a sensor adjacent to the pore configured to identify objects passing through the pore; and (c) determining, with the sensor, whether the fusion molecule or particle bound to the binding motif is bound to the target molecule or particle, thereby detecting the presence of the target molecule or particle in the sample.

Provided herein are encoded microcarriers for analyte detection in multiplex assays. The microcarriers are encoded with an analog code for identification and include a capture agent for analyte detection. Also provided are methods of making the encoded microcarriers disclosed herein. Further provided are methods and kits for conducting a multiplex assay using the microcarriers described herein.

Provided herein are encoded microcarriers for analyte detection in multiplex assays. The microcarriers are encoded with an analog code for identification and include a capture agent for analyte detection. Also provided are methods of making the encoded microcarriers disclosed herein. Further provided are methods and kits for conducting a multiplex assay using the microcarriers described herein.

Articles with covalently attached thiocarbonylthio-containing groups are provided. More specifically, the articles include a solid polymeric substrate with a plurality of thiocarbonylthio-containing groups covalently attached directly to a carbon atom in a polymeric backbone of the solid polymeric substrate. Methods of making the articles with covalently attached thiocarbonylthio-containing are provided. Additionally, methods of using these articles to generate further articles with covalently attached polymeric chains are provided.