A metal organic framework composite is provided. The composite includes a plurality of zinc ions, each coordinated with a salen ligand to form a salen complex metal-organic framework; and gold nanoparticles dispersed on a surface and pores of the salen complex metal-organic framework. Antibodies may be immobilized on a surface of the gold nanoparticles to be used in highly sensitive diagnostic methods for detecting and quantifying protein biomarkers such as human chorionic gonadotropin hormone.

The present invention relates to systems that utilize a combination of immunoassay and magnetic immunoassay techniques to detect an analyte within an extended range of specified concentrations. In particular, a device is provided for detecting an analyte in a biological sample. The device includes a first electrochemical sensor positioned on a substrate. The first electrochemical sensor includes an immobilized layer of antibody configured to bind to the analyte. The device further includes a second electrochemical sensor positioned adjacent to the first electrochemical sensor on the substrate, and a magnetic material that generates a magnetic field aligned with respect to the second electrochemical sensor. The magnetic field captures magnetic beads that have an immobilized layer of antibody configured to bind to the analyte, and concentrates the magnetic beads on or near a surface of the second electrochemical sensor.

The present invention relates to systems that utilize a combination of immunoassay and magnetic immunoassay techniques to detect an analyte within an extended range of specified concentrations. In particular, a device is provided for detecting an analyte in a biological sample. The device includes a first electrochemical sensor positioned on a substrate. The first electrochemical sensor includes an immobilized layer of antibody configured to bind to the analyte. The device further includes a second electrochemical sensor positioned adjacent to the first electrochemical sensor on the substrate, and a magnetic material that generates a magnetic field aligned with respect to the second electrochemical sensor. The magnetic field captures magnetic beads that have an immobilized layer of antibody configured to bind to the analyte, and concentrates the magnetic beads on or near a surface of the second electrochemical sensor.

The present invention relates to the field of Mycobacterium tuberculosis. More specifically, the present invention provides compositions and methods for detecting M. tuberculosis. In a specific embodiment, a method comprises the steps of (a) contacting a patient sample with a solid support coated with antibodies to carbapenem resistance factor A (CrfA); washing unbound molecules from the solid support using a buffer; and incubating the solid support with a beta-lactamase substrate. In certain embodiments, the beta-lactamase substrate is chromogenic. In another embodiment, the method further comprises the step of visually detecting a color change from the hydrolysis of the substrate by CrfA protein bound to the antibodies on the solid support. In yet another embodiment, the method further comprises the step of measuring color intensity of the strip at 490 nm using a spectrophotometer.

The present invention relates to the field of Mycobacterium tuberculosis. More specifically, the present invention provides compositions and methods for detecting M. tuberculosis. In a specific embodiment, a method comprises the steps of (a) contacting a patient sample with a solid support coated with antibodies to carbapenem resistance factor A (CrfA); washing unbound molecules from the solid support using a buffer; and incubating the solid support with a beta-lactamase substrate. In certain embodiments, the beta-lactamase substrate is chromogenic. In another embodiment, the method further comprises the step of visually detecting a color change from the hydrolysis of the substrate by CrfA protein bound to the antibodies on the solid support. In yet another embodiment, the method further comprises the step of measuring color intensity of the strip at 490 nm using a spectrophotometer.

Methods and kits for detecting and/or quantitating target proteins in biological samples. In particular, the method comprises capture and immobilization of the target protein, protein denaturation, proteolytic digestion, and analysis using a mass spectrometry-based technique.

Methods and kits for detecting and/or quantitating target proteins in biological samples. In particular, the method comprises capture and immobilization of the target protein, protein denaturation, proteolytic digestion, and analysis using a mass spectrometry-based technique.

A lateral flow test strip (LFTS) platform measures osteocalcin (OC) in saliva to identify early indications of bone loss and minimize bone fracture risk associated with osteoporosis. The OC assay embodiments are based on the experimentally identified optimal markers which exhibit selectivity with very low false positives, and sensitivity relevant to clinical requirements. A prospective clinical study sampling of 20 patients demonstrated excellent correlation of OC in saliva with bone mineral density (BMD). Salivary OC and Dpd levels were validated with a standard commercial ELISA kit against serum (OC) and urine (Dpd). Multiplexed LFTS are used to increase specificity of an assay.

A lateral flow test strip (LFTS) platform measures osteocalcin (OC) in saliva to identify early indications of bone loss and minimize bone fracture risk associated with osteoporosis. The OC assay embodiments are based on the experimentally identified optimal markers which exhibit selectivity with very low false positives, and sensitivity relevant to clinical requirements. A prospective clinical study sampling of 20 patients demonstrated excellent correlation of OC in saliva with bone mineral density (BMD). Salivary OC and Dpd levels were validated with a standard commercial ELISA kit against serum (OC) and urine (Dpd). Multiplexed LFTS are used to increase specificity of an assay.

The present invention provides a method for suppressing non-specific binding of a binding protein to a surface molecule of eukaryotic cell membrane or exosome, including immobilizing the binding protein on a carrier in the presence of gelatin.