The present invention relates to a nanoscreen for regulating stem cell adhesion and differentiation. Moreover, the present invention relates to a method of regulating stem cell adhesion and differentiation using the nanoscreen. According to the nanoscreen of the present invention and the method of regulating stem cell adhesion and differentiation using the same, it is possible to efficiently regulate stem cell adhesion and differentiation by applying a magnetic field to the nanoscreen.

Disclosed herein are methods for diagnosing or prognosticating SARS-CoV-2 infection and/or COVID-19 in a subject. The methods set forth improved immunoassays, sensing platforms, and methods for detecting SARS-CoV-2 infection and re-infection.

Disclosed herein are methods for diagnosing or prognosticating SARS-CoV-2 infection and/or COVID-19 in a subject. The methods set forth improved immunoassays, sensing platforms, and methods for detecting SARS-CoV-2 infection and re-infection.

The invention relates to a process for preparation of an unpassivated cantilever comprising the steps of: 1) providing a silicon cantilever sensor having two sides; 2) coating one side of the cantilever with at least a gold layer; and 3) functionalizing both sides of the cantilever with a self-assembled monolayer (SAM) of a probe molecule by incubating the cantilever in a solution having a concentration of the probe molecule of between 1 to 1000 μM.

The invention also relates to an unpassivated cantilever sensor comprising a silicon layer coated on one side with a coating comprising Au and being uncoated or unpassivated on the opposite side, wherein the Au coated surface comprises a self-assembled monolayer of a probe molecule and wherein the surface area occupied per probe molecule is in the range 0.4-1.5 nm.sup.2.

The invention relates to a process for preparation of an unpassivated cantilever comprising the steps of: 1) providing a silicon cantilever sensor having two sides; 2) coating one side of the cantilever with at least a gold layer; and 3) functionalizing both sides of the cantilever with a self-assembled monolayer (SAM) of a probe molecule by incubating the cantilever in a solution having a concentration of the probe molecule of between 1 to 1000 μM.

The invention also relates to an unpassivated cantilever sensor comprising a silicon layer coated on one side with a coating comprising Au and being uncoated or unpassivated on the opposite side, wherein the Au coated surface comprises a self-assembled monolayer of a probe molecule and wherein the surface area occupied per probe molecule is in the range 0.4-1.5 nm.sup.2.

A device and method for detecting and assessing the quantity of a biological, biochemical, or chemical analyte in a test sample using a simple light source and the naked eye are disclosed. In one embodiment, the device comprises a nanohole sensor chip with two sections, the first of which is a test section, upon which capture agents for a particular analyte are immobilized, and the second of which is a reference section, upon which capture agents conjugated with known quantities of the analyte are immobilized. In another embodiment of the invention, a nanohole sensor chip with a test section and a plurality of reference sections is disclosed. The sensor utilizes light intensity changes exhibited by Fano resonances in the nanoholes for detection of analytes, and allows comparison between the light intensity changes between the reference sections and the test sections for assessing the quantity of the analyte in the sample.

A device and method for detecting and assessing the quantity of a biological, biochemical, or chemical analyte in a test sample using a simple light source and the naked eye are disclosed. In one embodiment, the device comprises a nanohole sensor chip with two sections, the first of which is a test section, upon which capture agents for a particular analyte are immobilized, and the second of which is a reference section, upon which capture agents conjugated with known quantities of the analyte are immobilized. In another embodiment of the invention, a nanohole sensor chip with a test section and a plurality of reference sections is disclosed. The sensor utilizes light intensity changes exhibited by Fano resonances in the nanoholes for detection of analytes, and allows comparison between the light intensity changes between the reference sections and the test sections for assessing the quantity of the analyte in the sample.

A system and method for assaying high and low abundant biomolecules within a biological fluid sample is provided. The method includes: a) placing a biological fluid sample in contact with a first nanostructure surface; b) interrogating the sample with a light source, the sample in contact with the first nanostructure surface, the interrogation using a SERS technique; c) detecting an enhanced Raman scattering from at least one high abundant biomolecule type and producing first signals representative thereof; d) placing the sample in contact with a second nanostructure surface having a targeting agent that targets a low abundant biomolecule; e) interrogating the sample with the light source using the SERS technique; f) detecting the enhanced Raman scattering from the low abundant biomolecules and producing second signals representative thereof; and g) assaying the biological fluid sample using the first signals and the second signals.

A kit, system and method for the detection of a pathogen, in particular SARS-CoV-2, by surface enhanced Raman spectroscopy (SERS) obtained from a sample brought into contact with non-magnetic native metal nanoparticles. The kit includes the non-magnetic native metal nanoparticles and a software designed to detect the presence of the pathogen in the sample.

A kit, system and method for the detection of a pathogen, in particular SARS-CoV-2, by surface enhanced Raman spectroscopy (SERS) obtained from a sample brought into contact with non-magnetic native metal nanoparticles. The kit includes the non-magnetic native metal nanoparticles and a software designed to detect the presence of the pathogen in the sample.