Disclosed is a covalently-linked multilayered three-dimensional matrix comprising capture molecules, linkers and spacers (referred to as a Molecular Net) for specific and sensitive analyte capture from a sample. Also disclosed herein is a Molecular Net comprising covalently-linked multilayered three-dimensional matrix comprising more than one type of capture molecule and more than one type of linker and may comprise one or more spacer for specific and sensitive capture of more than one type of analyte from a sample. A Molecular Net may comprise a pseudorandom nature. Use of various capture molecules, linkers and spacers in a Molecular Net may confer unique binding properties to a Molecular Net. Porosity, binding affinity, size exclusion abilities, filtration abilities, concentration abilities and signal amplification abilities of a Molecular Net may be varied and depend on the nature of components used in its fabrication. Uses of a Molecular Net may include analyte capture, analyte enrichment, analyte purification, analyte detection, analyte measurement and analyte delivery. Molecular Nets may be used in liquid phase or on solid phases such as nanomaterials, modified metal surfaces, nanospheres, microspheres, microtiter plates, slides, pipettes, cassettes, cartridges, discs, probes, lateral flow devices, microfluidics devices, microfluidics devices, optical fibers and others.

Devices and methods for the detection of analytes are disclosed. Devices and methods for detecting food-borne pathogens are disclosed.

A device for performing a multiplex lateral flow immunoassay is provided in which a liquid sample, such as a biological sample, is simultaneously tested for the presence of multiple analytes of interest. Methods that employ the device in the simultaneous detection of multiple analytes of interest within a liquid test sample are also provided.

The present invention relates to a method for detecting food poisoning bacteria, and more particularly, to a method for rapidly and quantitatively isolating food poisoning bacteria contents which contaminate food and the like. The method according to the present invention is characterized by including the steps of: introducing magnetic nanoparticles which can bind to bacteria into a sample for measuring the bacteria so as to bind the magnetic nanoparticles to the bacteria; isolating the magnetic nanoparticles; passing the nanoparticles which are isolated by using magnetism through a solution having high viscosity so as to separate the magnetic nanoparticles to which bacteria are bound from magnetic nanoparticles to which no bacteria are bound; and quantifying the magnetic nanoparticles to which bacteria are bound.

Pseudomonas aeruginosa flagellin protein recruits the mammalian host sialidase enzyme neuraminidase-1 (NEU1) to remove sialic acid residues from the extracellular domain of the mammalian cell-surface protein MUC1 (MUC1-ED), thereby exposing a cryptic binding site on the MUC1-ED protein backbone for flagellin binding. NEU1-driven MUC1-ED desialylation rapidly increases P. aeruginosa adhesion to the airway epithelium. MUC1-ED desialylation also increases MUC1-ED cleavage and shedding from the cell surface, where desialylated, shed MUC1-ED competitively blocks P. aeruginosa adhesion to cell-associated MUC1-ED. Presented herein are data showing that exogenously-administered, deglycosylated MUC1-ED peptides reduced adhesion of P. aeruginosa to airway epithelial cells. Also presented are data showing that administration of P. aeruginosa to mice in combination with deglycosylated MUC1-ED decreased P. aeruginosa recovered from the lungs at 48 hr and 72 hr post-infection. Such findings are extended to the methods of treatment and prevention of bacterial infections defined herein.

An object of the present invention is to elucidate the cause of degradation of the reliability of detecting causative bacteria of environmental mastitis such as Escherichia coli, Klebsiella bacteria, Streptococcus bacteria, and CNS, and to provide a highly reliable detection means. Another object of the present invention is to provide a highly reliable detection means based on the immunochromatographic method, which enables quicker determination compared with the cultivation-based method, and a diagnosis kit using it. The present invention provides a method for detecting a causative bacterium of environmental mastitis of a livestock animal, which comprises: the step of determining whether number of the causative bacterium in a milk of a subject livestock animal is not smaller than a determination value defined beforehand on the basis of distribution of amounts of the causative bacterium in milks derived from a non-disease group and distribution of the amounts of causative bacterium in milks derived from a disease group by an immunological test method, and wherein the immunological test method is an immunochromatographic method using an antibody labeled with not less than 0.6/cm.sup.2 and not more than 3.5/cm.sup.2 of gold colloid particles (particle-labeled antibody).

A method, device and kit for detecting sepsis or bacteremia in a patient includes detecting peptidoglycan associated lipoprotein (Pal) from Gram-negative bacteria in the urine of the patient is disclosed.

The disclosure provides methods, compositions, and kits for enhanced detection of microbes in samples and monitoring of antimicrobial activity in a subject.

The present invention relates to a synthetic oligosaccharide of general formula (I): T*-[(—U.sub.x+4—U.sub.x+3—U.sub.x+2—U.sub.x+1—U.sub.x).sub.m—(V.sub.x+2—V.sub.x+1—V.sub.x).sub.1-m-T-O-L-E that is related to Klebsiella pneumoniae serotype O3, O3b and/or O5 lipopolysaccharide and conjugate thereof. Said synthetic oligosaccharide, said conjugate and pharmaceutical composition containing said synthetic oligosaccharide or said conjugate are useful for prevention and/or treatment of diseases associated with Klebsiella pneumoniae. Furthermore, the synthetic oligosaccharide of general formula (I) is useful as marker in immunological assays for detection of antibodies against Klebsiella pneumoniae serotype O3, O3b and/or O5 bacteria.

Disclosed is information related to determining Escherichia coli from a sample such as urine. According to the method, part of sample is admixed with a reagent including a lanthanide(III) ion, a transition metal ion, and a transition metal ion/E. coli-specific M13 phage, and another part of the sample is admixed with the reagent including lanthanide(III) ion, the transition metal ion a wild-type M13 phage. The signals derived from the lanthanide(III) ions in the admixtures were detected with time-gated luminescence measurement. The presence of E. coli in the sample was determined by comparing the lanthanide(III) ion signal in the presence transition metal ion/E. coli-specific M13 phage and the wild type M13 phage.