This present disclosure provides methods and compositions that can be used to quantify cfDNA in biofluids using a hybridization approach.

The invention discloses a detergent-compatible protein assay method, composition and kit based on bio-conjugation reaction between protein and Meldrum's acid activated furfural. The method includes adding MAF in dimethyl sulfoxide (DMSO) to a protein sample solution. The amine functionalities present on the amino acid residues reacts with the MAF instantaneously at room temperature to yield deep purple colored solutions of the corresponding conjugated proteins. The reagent composition added to protein may be in the range of 90-450 mM. The intensities of purple colored solutions were proportional to the protein concentration captured by spectrophotometric measurements. The assay is sensitive in the range of 0.125-15 mg/mL, is compatible with commonly used detergents and reducing agents in protein solutions and may be employed for estimation of protein samples in the presence of detergents and reducing agents.

This disclosure relates generally to novel kits for measuring insect health, and to methods of making and using such compositions. More specifically, the invention relates to novel kits for a rapid and high-throughput measurement of lipase activity levels in insects, and the correlation of the measured lipase activity levels with insect stress.

Method, kit and composition for analyzing analytes for modifications using modification site specific antibodies to bind an analyte with his specific modification sites of interest to different dyes simultaneously with an antibody which is specific to the non-modificated analyte binding to another dye to determine the concentration of the analyte for quantification of the modified analyte in the identical sample.

Provided herein is the use of measurements of cell-free DNA, protein, and/or metabolite found in biofluid (e.g., urine) for identifying and treating organ injury. Provided herein are methods and compositions for monitoring, detecting, quantifying, and treating kidney injury in subjects suffering from or suspected of having an altered renal status by measuring amounts of cfDNA and one or more other markers, such as inflammation markers, apoptosis markers, protein, and DNA methylation.

This application relates to the use of measurements of cell-free DNA, protein, and/or metabolite found in biofluid (e.g., urine) for identifying and treating organ injury. The application includes methods and compositions for monitoring, detecting, quantifying, and treating kidney injury in subjects suffering from or suspected of having an altered renal status by measuring amounts of cfDNA and one or more other markers, such as inflammation markers, apoptosis markers, protein, and DNA methylation.

The present invention provides use of quinaldine red (QR) and/or a derivative thereof in preparation of a kit for detecting amyloid fibrils. The derivative includes 4-(4-dimethylaminostyryl)quinoline, and the present invention belongs to the technical field of amyloid fibril detection. Compared with traditional amyloid fibril detection probes, the QR and its derivative 4-(4-dimethylaminostyryl)quinoline have higher binding constants for binding with the amyloid fibrils; and good photobleaching performances; and in the present invention, after combined with the amyloid fibrils, both the QR and its derivative 4-(4-dimethylaminostyryl)quinoline have a fluorescence emission wavelength range that is close to a far-infrared band, and thus are more suitable for imaging a pathological tissue of a biological tissue.

A method of measuring the harshness of a surfactant, comprising the steps of: i) preparing a solid protein-dye complex comprising: a) a protein, which is a non-denatured corn protein and which is soluble in aqueous alcohol; and b) a protein binding dye, which is specific to the protein (a); by dissolving a) and b) in aqueous alcohol to form a solution of protein-dye complex; and removing the aqueous alcohol to form a solid protein-dye complex; and ii) providing an aqueous solution of surfactant and taking a first colour measurement, iii) adding the solid protein-dye complex to the aqueous solution of surfactant, taking a second colour measurement and measuring the change in colour between the first colour measurement and the second colour measurement; and iv) matching the change in colour with a reference scale; provides a quick and accurate way of assessing surfactant harshness towards protein, that can be easily carried out under non-laboratory conditions and enables suitable product recommendations to be made.

This application relates to the use of measurements of cell-free DNA, protein, and/or metabolite found in biofluid (e.g., urine) for identifying and treating organ injury. The application includes methods and compositions for monitoring, detecting, quantifying, and treating kidney injury in subjects suffering from or suspected of having an altered renal status by measuring amounts of cfDNA and one or more other markers, such as inflammation markers, apoptosis markers, protein, and DNA methylation.

Peptide and/or protein quantitation methods, kits, and compositions, particularly useful for mass spectrometry, are provided herein based on a bathocuproine-based composition complex such as bathocuproinedisulfonic acid disodium salt hydrate complex. The methods are one-step rapid absorbance methods using small sample volumes. They produce a robust signal with high signal to background ratio and accurately quantitate even complex peptide mixtures with low variability and high sensitivity.