Method, kit and composition for analyzing analytes for modifications using modification site specific antibodies to bind an analyte with his specific modification sites of interest to different dyes simultaneously with an antibody which is specific to the non-modificated analyte binding to another dye to determine the concentration of the analyte for quantification of the modified analyte in the identical sample.

The present invention relates to a stable protein and/or amino acid detecting composition that can be used as a reagent for in situ detection, such as on surfaces. The invention also relates to a method for detecting protein and/or amino acid on surfaces using the composition and kits comprising the composition.

Methods and systems for identifying capsid viral proteins in a sample containing viral vectors are provided, including determining the ratio of the capsid viral proteins of adeno-associated virus. The methods and systems comprise denaturing the capsid viral proteins in the sample, labelling the denatured capsid viral proteins with a lysine-conjugation dye, generating a separation profile of the denatured/labelled capsid viral proteins using microchip capillary electrophoresis, quantifying levels of the capsid viral proteins based on the separation profile, determining a quantification ratio of the capsid viral proteins based on the separation profile, and normalizing the quantification ratio based on lysine contents of the capsid viral proteins.

The kit for quantitative analysis of Glycated Albumin (GA) according to an embodiment of the present application comprises: a first composition including a reaction buffer for inducing binding with albumin present in a blood sample; a second composition including a decomposition reagent for a decomposition reaction to break down glycated albumin (GA) included in the blood sample into glycated amino acids; a third composition including a reaction reagent for an enzymatic reaction of the glycated amino acids present in the blood sample; and a fourth composition including a chromogenic agent being oxidized by hydrogen peroxide (H.sub.2O.sub.2) generated from the enzymatic reaction of the glycated amino acids.

Methods and systems for identifying capsid viral proteins in a sample containing viral vectors are provided, including determining the ratio of the capsid viral proteins of adeno-associated virus. The methods and systems comprise denaturing the capsid viral proteins in the sample, labelling the denatured capsid viral proteins with a lysine-conjugation dye, generating a separation profile of the denatured/labelled capsid viral proteins using microchip capillary electrophoresis, quantifying levels of the capsid viral proteins based on the separation profile, determining a quantification ratio of the capsid viral proteins based on the separation profile, and normalizing the quantification ratio based on lysine contents of the capsid viral proteins.

Methods of minimizing hook effect interference in an immunoassay are disclosed. Also disclosed are reagents, kits, and immunoassay devices that may be utilized in accordance with the method.

A staining kit is provided, including a first pattern including antibodies against T cell, B cell, NK cell, monocyte, regulatory cell, CD8, CD45, and CTLA4; a second pattern including antibodies against T cell, B cell, NK cell, monocyte, regulatory cell, dendritic cell, and CD45; a third pattern including antibodies against T cell, B cell, NK cell, monocyte, CD8, CD45, CD45RA, CD62L, CD197, CX3CR1 and TCR.sub.; and a fourth pattern including antibodies against B cell, CD23, CD38, CD40, CD45 and IgM, wherein the antibodies of each pattern are labeled with fluorescent dyes. A method of identifying characterized immune cell subsets of a disease and a method of predicting the likelihood of NPC in a subject in the need thereof using the staining kit are also provided.

The invention provided herein relates to methods for protein synthesis, purification and characterisation.

Methods of minimizing hook effect interference in an immunoassay are disclosed. Also disclosed are reagents, kits, and immunoassay devices that may be utilized in accordance with the method.

An object of the present invention is to provide a test piece for measuring albumin, which is not affected by globulin or coexisting substances in serum, have high measurement accuracy, and have good repeatability, and to provide a method for measuring albumin.

According to the present invention, there is provided a test piece for measuring albumin, including a support and a reagent holding layer provided over the support, in which the reagent holding layer contains a protein denaturant, an SH reagent, bromocresol purple, and a nonionic surfactant, a content of the bromocresol purple in the reagent holding layer is 0.78 g/m.sup.2 to 3.81 g/m.sup.2, and a ratio of a content of the nonionic surfactant to the content of the bromocresol purple is 0.01 to 0.3.