Provided is biological magnetic microsphere, comprising magnetic microsphere body. Magnetic microsphere body comprises, on outer surface thereof, at least one polymer having linear backbone and side chain. End of linear backbone fixes to outer surface of magnetic microsphere body, other ends of polymer unattach to outer surface of magnetic microsphere body. Biotin links to terminal end of side chain of polymer of biological magnetic microsphere. Further provided are modification to, preparation method for, use of biological magnetic microsphere. Biological magnetic microsphere can be handled and used conveniently, rapidly disperse and rapidly precipitate in solution without need to use large-scale experimental equipment such as high-speed centrifuge, can be connected via biotin with purification element having selectivity (such as avidin, affinity protein, polypeptide/protein tag, etc.), is versatile in application and can be widely and massively used in separation and purification of target substance, such as protein, including but not limited to antibody.

In at least one illustrative embodiment, a system may include a basin that includes an index plate positioned at a bottom of the basin. The basin is configured to receive a liquid analyte, such as a liquid food product or a nutrient broth. The index plate includes an array of multiple wells. Each well opens into an interior of the basin and is sized to receive a magnetostrictive sensor in a predetermined orientation. One or more sensor coils is positionable beneath each well. The basin may be filled with liquid analyte and magnetostrictive sensors may be positioned in the wells. The liquid analyte may be allowed to incubate at a controlled temperature. A controller may position a sensor coil beneath a well, apply a varying magnetic field to a magnetostrictive sensor in the well, and detect a frequency response of the magnetostrictive sensor. Other embodiments are described and claimed.

The present invention relates to cell-based methods for screening body fluids or tissues for factors that prevent cellular uptake of lysosomal enzymes, including neutralizing factors such as neutralizing antibodies, that arise as a result of lysosomal enzyme replacement therapy.

A method for magnetic cellular manipulation may include contacting a composition with a biological sample to form a mixture. The composition may include a plurality of particles. Each particle in the plurality of particles may include a magnetic substrate. The magnetic substrate may be characterized by a magnetic susceptibility greater than zero. The composition may also include a chargeable silicon-containing compound. The chargeable silicon-containing compound may coat at least a portion of the magnetic substrate. The biological sample may include cells and/or cellular structures. The method may also include applying a magnetic field to the mixture to manipulate the composition.

Coated Ferromagnetic Density Particles or Density Particles with binding agents bound thereto capable of binding biological molecules and methods of use and apparatus for means are disclosed. Coated particles coupled to specific binding agents can be used for molecular biology and proteomic applications in research and diagnostics.

A novel system for the analysis of molecules and cells, comprising clusters where a non-magnetic particle is supplemented with magnetic particles to form a characteristic pattern, fingerprint or bar code. Methods and devices for formation of such particles are also disclosed.

Provided is a magnetic particle excellent in detection speed when a substance to be measured, such as an antigen or an antibody, is detected from a specimen. The particle includes a magnetic particle containing a magnetic material, wherein the magnetic particle has a resin on a surface, wherein the particle has a volume average particle diameter of 0.4 μm or more and 1.5 μm or less, wherein the particle has a density of 5.1 g/cm.sup.3 or more and 10.0 g/cm.sup.3 or less, and wherein the resin has a functional group capable of binding a ligand.

This present disclosure relates to the use of donor organ-derived microvesicles to monitor the status of a transplanted organ in a subject. Accordingly, this disclosure provides for methods and kits for isolating, purifying and/or identifying donor organ-derived microvesicles from a biological sample of a subject. In certain embodiments, a method for isolating, purifying and/or identifying donor organ-derived microvesicles includes obtaining a biological sample from the subject and isolating, purifying or identifying a donor organ-derived microvesicle from the biological sample by the detection of a protein specific for the donor.

Disclosed probes comprise metal nanoparticle cores associated with magnetic particles that allow probes associated with targets to be concentrated by an applied magnetic field to increase detection sensitivity and provide sufficient spacing between concentrated probes to avoid signal quenching. The probe may comprise at least one recognition receptor, and may further comprise at least one reporter molecule, such as a fluorescent tag, a Raman reporter, or combinations thereof. Concentrating probe-target composites substantially enhances a sensing signal, such as from 5 to 10 times, compared to detection without concentrating the probes. The method may be used to detect, for example, interleukins at concentrations at least as low as 25 pg/ml in sputum or blood from a subject for early and precise profiling of viral infections, such as SARS-CoV-2 infections.

A method of manufacturing a polymer coated single ferromagnetic particle is provided. The method includes hydrophobizing one single hydrophilic ferromagnetic particle by absorbing an aliphatic acid having a hydrophobic aliphatic group and a hydrophilic acid group onto the single ferromagnetic particle to obtain a single hydrophobic ferromagnetic particle; emulsifying the one single hydrophobic ferromagnetic particle with a monomer liquid comprising a nonionic surface activating agent which re-hydrophilizes the one single hydrophobic ferromagnetic particle, to obtain an emulsified liquid; adding a radical addition initiator to the emulsified liquid; and emulsion polymerizing the monomer by radical addition polymerization.