The present invention provides assay devices and methods for detecting the presence of an analyte in a sample. Devices according to the invention include a reagent zone, one or more capture zones and a detection zone. Capture zones can reduce the quantity of labelled conjugate that reaches the detection zone in the presence of a negative marker in the sample and/or in the absence of a positive marker in the sample, facilitating high sensitivity and improved specificity testing.

The invention aims to suppress non-specific reaction effectively in an immunochromatographic assay method for an analyte. The object can be achieved by an immunochromatographic assay method for an analyte, the method including using a test strip including the following (1) to (3): (1) a sample pad containing a sample application region for applying a biological sample that may contain the analyte; (2) an insoluble membrane containing at least one detection region to which a binding partner immunologically reactive with the analyte is immobilized, wherein the binding partner is immobilized to a label; and (3) a conjugate containing a binding partner immunologically reactive with the analyte, wherein the binding partner is immobilized to a label.

The invention aims to carry out an immunoassay method for a respiratory tract virus, wherein non-specific reaction is suppressed. The object can be achieved by an immunoassay method for a respiratory tract virus, the method comprising: (A) bringing a biological sample that may contain a respiratory tract virus into contact with an anionic surfactant; (B) bringing the respiratory tract virus into contact with a conjugate, to forma first complex; (C) bringing the first complex into contact with an antibody immunologically reactive with the respiratory tract virus, to forma second complex; and (D) measuring a signal derived from the conjugate.

Provided are: a coloring cellulose microparticle enabling false positive to be significantly reduced while maintaining a high detection sensitivity; and an immunochromatographic diagnostic kit using the same.

The present disclosure relates to a kit for quantitative detection using a fluorescent microarray, and belongs to the technical field of protein detection. The kit of the present disclosure includes a detection plate and a detection antibody coupled with fluorescent microspheres, where the detection plate is provided with a plurality of reaction chambers; the reaction chamber is provided with an opening, and an inner bottom surface of the reaction chamber is provided with a plurality of detection sites that are arranged side by side along a length direction of the reaction chamber at an interval. The kit of the present disclosure may detect allergen-specific IgE, IgG and IgA with high sensitivity, as well as rapidly and quantitatively detect an allergen-specific antibody IgE, IgG and IgA concentration in human serum or plasma, and may screen dozens of allergens at a time.

An object is to strongly suppress a false negative reaction and a false positive reaction, which cannot be sufficiently suppressed by a conventional method, in detection of an antigen such as a virus, a bacterium, or a protein to be detected in a specimen originated from a body fluid such as a nasal swab specimen, a nasal aspirate specimen, a nasal wash specimen, a blown snot specimen, a pharyngeal swab specimen, or a saliva specimen with a detection reagent utilizing an antigen-antibody reaction or a reaction between substances interacting with each other. The present invention provides a test reagent for detecting a target substance in a specimen by utilizing an antigen-antibody reaction or a binding reaction between substances interacting with each other, comprising a specimen extracting solution containing a chelating agent.

The present invention discloses a method for testing different hormones based on different time periods of pregnancy preparation, including four method steps: fertility test, ovulation test, ovulation confirmation test and pregnancy test. The present invention provides a set of systematic and perfect pregnancy preparation management plan for pregnancy preparation women, which can provide reasonable and effective test guidance for pregnancy preparation women, helps pregnancy preparation women to determine and analyze various hormone test results, and gives corresponding suggestions based on the test results to better help women complete the pregnancy preparation process. The present invention comprehensively and systematically analyzes and determines various hormone levels of women during pregnancy preparation, with high accuracy and reliable analysis results. This pregnancy preparation management method, combined with a hormone test device, enables pregnancy preparation women to complete various hormone tests at home and analyze the test results without going to the hospital frequently, has little impact on life, and brings convenience to the pregnancy preparation women.

The present invention provides a method for preparing colloidal palladium nanoparticles and using them for increased sensitivity in lateral flow immunoassays. Glutaraldehyde is used in preparing the colloidal palladium that allows rapid attachment of biomolecules. Colloidal palladium nanoparticles are labeled with a protein, such as a biomolecule or an antibody. These labeled colloidal palladium particles catalytically develop a dye to detect the presence of an analyte.

An apparatus comprising a mobile computing device (902) physically coupled to a lightbox (904). The apparatus includes camera hardware, processing circuitry in communication with the camera hardware, and an interface in communication with the processing circuitry. The camera hardware is configured to capture image data associated with an output signal area of a biological chromatographic test strip (410) inserted into a receiving slot of the lightbox. The processing circuitry is configured to determine, based on the image data captured by the camera hardware, a concentration of a target analyte in a test sample submitted via the biological chromatographic test strip. The interface is configured to output data indicative of the concentration of the target analyte determined by the processing circuitry.

An immunoassay device that authenticates a biological sample while performing an assay for an analyte or analytes of interest is provided. The device includes a sample receiving zone to receive the biological sample; a validation zone placed before or after the sample receiving zone to validate the biological sample; a conjugate zone having labels conjugated with primary antibodies or reagents specific to a plurality of characteristic markers of the biological sample and the analytes of interest; and a reaction zone having secondary antibodies or antigens or reagents specific to the primary antibodies or reagents that bind with the plurality of characteristic markers of the biological sample and analytes of interest.