High-frequency irreversible electroporation (H-FIRE) is a tissue ablation modality employing bursts of electrical pulses in a positive phase-interphase delay-negative phase-interpulse delay pattern. Despite accumulating evidence suggesting the significance of these delays, their effects on therapeutic outcomes from clinically-relevant H-FIRE waveforms have not been studied extensively. The present invention provides methods of pulse delivery, including delays, that mitigate bubble formation and/or minimize the risk of arcing, such as due to the presence of bubbles, and/or minimize muscle stimulation are described herein.

Products of manufacture for delivering hydrogen, hydrogen peroxide, and/or oxygen to a tissue in need thereof comprising i) a hydrated hydrogel, and ii) at least one cathode electrode and at least one anode electrode implanted within or upon the hydrated hydrogel.

In alternative embodiments, provided are products of manufacture and kits, and methods, for hydrogen and hydrogen peroxide generation, and for the treatment of diseases, conditions and infections responsive to exposure to a therapeutic amount of hydrogen, metastable hydrogen peroxide and/or oxygen treatment; including providing a hydrogen, metastable hydrogen peroxide and/or oxygen treatment or delivery system for cosmetic purposes.

A locator assembly (100) for determining a location of an arrhythmogenic foci (632) in or near a heart (101). The locator assembly (100) having a longitudinal axis (100a) and a circumference (100c). The locator assembly (100) includes a device body (112) and a plurality of components that are coupled to the device body (112). The plurality of components are distributed about the longitudinal axis (100a) and the circumference (100c). The device body (112) is movable between (i) a contracted state wherein the device (112) body is contracted, and (ii) an expanded state wherein the device body (112) is expanded outwardly from the contracted state so that the device body (112) has an increased circumference (100c).

A locator assembly (100) for determining a location of an arrhythmogenic foci (632) in or near a heart (101). The locator assembly (100) includes a device body (112) and a plurality of electrodes (102). The plurality of electrodes (102) receive electrical signals from the heart (101) to determine the location of the arrhythmogenic foci (632). The plurality of electrodes (102) can be coupled to the device body (112). At least two of the plurality of electrodes (102) can positioned circumferentially about the device body (112). The plurality of electrodes (102) can be positionable so that the plurality of electrodes (102) are in electrical communication with the heart (101).

High-frequency irreversible electroporation (H-FIRE) is a tissue ablation modality employing bursts of electrical pulses in a positive phase-interphase delay-negative phase-interpulse delay pattern. Despite accumulating evidence suggesting the significance of these delays, their effects on therapeutic outcomes from clinically-relevant H-FIRE waveforms have not been studied extensively. The present invention provides methods of pulse delivery, including delays, that mitigate bubble formation and/or minimize the risk of arcing, such as due to the presence of bubbles, and/or minimize muscle stimulation are described herein.