Devices and methods for determining activity of one or more coagulation factors in a blood sample are provided. The device may comprise an inlet port for deposition of a sample, a reaction compartment, a detection compartment, a control compartment, or any combination thereof. One or more compartments may be fluidically connected. One or more compartments may comprise plasma deficient of a coagulation factor, an ionic citrate source, an ionic calcium source, one or more coagulation contact phase activator reagents, a phospholipid, or a mixture, or any combination thereof.

The application relates to a sample holder (110) and a system (100). The application also relates to a method for processing a biological sample (S) and use of the sample holder or of the system in an analytical method or a diagnostic method. The sample holder (110) comprises a tubular member (111) with a wall that is at least locally transparent and at least locally permeable for reagents, wherein the tubular member consists at least partially of a transparent material.

A microfluidic chip orients and isolates components in a sample fluid mixture by two step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.

A solid reagent containment unit is formed by a support; a frame body fixed to the support and delimiting internally, together with the support, an analysis volume; a reagent-adhesion structure within the analysis volume; and at least one reagent cavity, which extends within the reagent-adhesion structure. The reagent-adhesion structure is of an adhesion material embossable at temperatures lower by 6-8° C. than its own melting point and has a melting point such as not to interfere with the analysis. The reagent cavity forms a retention wall, laterally surrounding the reagent cavity, and houses dried reagents. The adhesion material is chosen among wax, such as paraffin, a polymer, such as polycaprolactone, a solid fat, such as cocoa butter, and a gel, such as hydrogel or organogel.

An apparatus, system, and method for filtering and assaying a fluid sample are described. In an embodiment, the apparatus includes a filtration unit comprising: a filter bracket shaped to removably couple with a fluid sample cup and a vacuum container; and a filter housing cooperatively couplable to the filter bracket and comprising a filter configured to filter fluid passing through the filter bracket; and an assay device shaped to cooperatively couple with the filter housing and comprising a porous matrix positioned to be in fluidic communication with the filter when the filter housing is cooperatively coupled with the assay device.

The present disclosure relates to a biological test cassette and a medical test system, the biological test cassette comprising a support, a cassette body, a chip, a first connecting tube and a second connecting tube. The support is provided with a first via hole. The cassette body is provided with a second via hole corresponding to the position of the first via hole, mounted on a top surface of the support, and further provided with a pretreatment chamber and a first flow channel in communication with the pretreatment chamber. The chip is slidably provided on a bottom surface of the support, and provided with an analyzing and processing chamber and a second flow channel in communication with the analyzing and processing chamber. The second connecting tube can be freely bent and deformed during the process of pulling the chip out from or pushing the chip back into the support.

There is provided a microfluidic chip for sensing an analyte in a sample by colorimetry. The microfluidic chip comprises: an inlet adapted to receive the sample; an incubation chamber having an incubation chamber inlet fluidly connected to the inlet downstream thereof, to incubate the analyte in the sample; a filter barrier fluidly connected to the incubation chamber, downstream of the incubation chamber inlet; a sensing chamber fluidly connected to the incubation chamber, downstream of the filter barrier, the sensing chamber having a plasmonic nanosurface, the plasmonic nanosurface including nanostructures protruding from the plasmonic nanosurface, the nanostructures having a size that is smaller than that of the diffraction limit of light, the nanostructures having a metallic layer that is plasmon-supported on top of a back reflector layer; and an outlet fluidly connected to the sensing chamber downstream thereof.

The embodiments disclose a test cartridge assembly system including a test cartridge assembly for loading of a test sample moving the fluid through the internal fluidic channels processing and presenting the sample to one or more electrochemical sensors for measuring analytes in the test sample, at least one fluidic channel formed directly in a rigid portion of an upper test cartridge assembly housing to form a fluidic path, a port coupled to the at least one fluidic channel for receiving a spring loaded vacuum source, a port coupled to the upper test cartridge assembly housing to communicate the vacuum through the upper cartridge housing into the fluidic path, and a functional layer coupled to the test cartridge assembly configured to provide electrical functionalities and interconnections to various fluidic components.

The present disclosure relates to a mechanism for fluidic sealing of a reaction cartridge in a reaction system using a single linear actuator. A single motion provided by the linear actuator is used to establish leak-resistant fluid communication between the reaction cartridge and two independent fluidic channels. The dual-sealing assembly described herein enables the use of fewer parts and a simpler control unit. The use of fewer parts and simpler control system allow for a very compact sealing mechanism and could also increase reliability, will be easier to manufacture as it will require less manufacturing testing and calibration, and is more tolerant of variance in the part being sealed (the reaction cartridge). In some embodiments, the reaction cartridge comprises a solid support matrix and a reaction reagent attached to the solid support matrix, and the reaction system is used for treating macromolecules, such as polypeptides, for sequencing and/or analysis.

A solid reagent containment unit is formed by a support; a frame body fixed to the support and delimiting internally, together with the support, an analysis volume; a reagent-adhesion structure within the analysis volume; and at least one reagent cavity, which extends within the reagent-adhesion structure. The reagent-adhesion structure is of an adhesion material embossable at temperatures lower by 6-8° C. than its own melting point and has a melting point such as not to interfere with the analysis. The reagent cavity forms a retention wall, laterally surrounding the reagent cavity, and houses dried reagents. The adhesion material is chosen among wax, such as paraffin, a polymer, such as polycaprolactone, a solid fat, such as cocoa butter, and a gel, such as hydrogel or organogel.