The extraction system according to the invention for extracting analytes from a sample comprises an extraction cell for receiving the sample, which has at least one first fluid connector for the supply or discharge of a fluid, and a clamping device for clamping the extraction cell, and a fluid connection device for establishing a fluid connection with the at least one first fluid connector of the extraction cell,
wherein an extraction cell holder is provided for receiving the extraction cell, and the clamping device has a guiding and positioning arrangement for linearly inserting the extraction cell holder, together with the extraction cell, in order to position the extraction cell in an insertion position.

A microfluidic device includes a first substrate including at least one microfluidic channel and a plurality of microwells, as well as a cooperating second substrate defining multiple split-walled cell trap structures that are registered with and disposed within the plurality of microwells. A method for performing an assay includes flowing cells and a first aqueous medium into a plurality of microwells of a microfluidic device, wherein each microwell includes a cell trap structure configured to trap at least one cell. The method further comprises flowing a nonpolar fluid with low permeability for analytes of interest through a microfluidic channel to flush a portion of the first aqueous medium from the microfluidic channel while retaining another portion of the first aqueous medium and at least one cell within each microwell. Surface tension at a non-polar/polar medium interface prevents molecule exchange between interior and exterior portions of microwells.

The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest.

A method of nucleic acid extraction utilizes small magnetizable beads (e.g., iron, cobalt, nickel, etc.) that react to the presence of a magnetic field by participating in a paramagnetic flux. The magnetizable beads clump together in a mass inside of a reaction vessel. The reaction vessel can be different shapes and sizes, but it is typically a cylindrical test tube. A magnet is positioned proximal to the reaction vessel with the beads inside. By rotating the magnet around the tube (or spinning the tube), the beads will follow the magnet around the tube due to the magnetic flux, creating a grinding or macerating effect while mixing the contents. When biological samples are also placed into the reaction vessel with the magnetizable beads, they become homogenized in a solution in the reaction vessel as the magnet moves around the vessel.

A system for collecting a biological sample from a passenger cabin includes a collector for collecting particulate samples positioned within at least one of an outlet flow path or a recirculation flow path. The system includes at least one of an outflow valve positioned in the outlet flow path downstream from the collector or a HEPA (high efficiency particulate air) filter positioned in the recirculation flow path downstream from the collector. A method for collecting particulates from cabin air includes capturing particulates in at least one of an outlet flow path or a recirculation flow path with a collector for a period of time, removing the collector from at least one of the outlet flow path or the recirculation flow path for testing, and inserting a clean collector into at least one of the outlet flow path or the recirculation flow path for use during another period of time.

The present invention relates to the field of assay devices and methods for performing assays, such as immunoassays. The system comprises a means to receive the target sample collected from a subject; a means to measure the target analyte possibly present in said target sample; and a process of measuring the target analytes in real time manner. The target analyte includes but is not limited to any biological analyte, microbial entity like those of viral or bacterial sources such as SARS-CoV-2. The invention thus primarily relates to measuring analytes of interest to detect and treat related indications, such as COVID-19.

Disclosed herein is a single-cell analysis workflow involving whole genome amplification for developing single-cell whole genome DNA libraries. The single-cell analysis workflow involves encapsulating and lysing cells in individual droplets and releasing genomic DNA from chromatin within the droplet. Transposases access the released genomic DNA and insert adaptor sequences into the cleaved nucleic acid fragments, thereby generating tagmented genomic DNA fragments that span the whole genome. Tagmented genomic DNA undergo nucleic acid amplification and sequencing for generating single-cell whole genome DNA libraries.

A thermal analysis of samples and proposes a device for a thermal analysis of a sample, having: a sample chamber for receiving a sample crucible including a crucible cover attached thereto, in the interior of which a sample to be analyzed is located, wherein the sample chamber has a chamber opening for introducing the sample crucible into the sample chamber; a temperature control mechanism for controlling the temperature of the sample chamber; a measuring mechanism for measuring a temperature of the sample and one or several further measured variables; a gas conveying mechanism for creating a gas atmosphere in the sample chamber; a chamber cover, which can be attached to the chamber opening of the sample chamber; and a piercing mechanism equipped with a needle, which is suitable to pierce a hole into the crucible cover of the sample crucible by means of the needle when the sample crucible is received in the sample chamber and when the chamber cover is attached to the chamber opening. A corresponding method for a thermal analysis of a sample, a sample crucible including a crucible cover, as well as a covering/piercing unit are further proposed as part of the invention.

Disclosed herein are devices and methods for contacting a tissue sample with liquids to perform a variety of processes and analyses. In various embodiments, the device includes a hydrophilic flow path defined at least in part by a surface pattern (e.g., bounded by hydrophobic regions). The flow path includes a sample area sized to receive a biological sample. The flow path includes at least one valve configured to control flow in the flow path in response to an external stimulus.

Aspects of this disclosure relate to systems that include a channel with at least one fluid in particle in fluid. The at least one particle can move along a defined path of the channel in response to a magnetic field. At least one structure is integrated with the channel, such as a sensor to generate an output signal related to the magnetic field or a magnetic structure to apply the magnetic field. Relates methods are also disclosed.