The present invention relates to a method of manufacturing an animal cartilage-derived injectable composition, an injectable composition manufactured by the method, and a use thereof.

The injectable composition of the present invention includes a collagen-containing biomaterial in a formulation injectable into joint cavity and may induce cartilage tissue regeneration by repairing tissue via direct injection to a target region without surgical incision. In addition, the injectable composition may be used as a therapeutic agent for arthritis by alleviating osteoarthritis not only because the animal cartilage-derived extracellular matrix contained in the injectable composition protects articular cartilage tissue but also because an environment capable of regenerating damaged articular tissue is created by inducing differentiation of intra-articular stem cells into chondrocytes.

A composition has hyaluronic acid and one or more materials as an admixture. The hyaluronic acid is derived from a fascia tissue layer of an alligator, the fascia layer located below a hide and above muscle tissue. The one or more materials as the admixture to the hyaluronic acid can be a carrier, diluent or excipient. The hyaluronic acid is extracted from the fascia tissue layer in the form of an oil having an oily viscosity with a molecular weight of 30,000 or greater. The oil extracted includes the hyaluronic acid and includes sodium or salts of hyaluronic acid. The oil extracted is anti-inflammatory to human tissue.

Therapeutic devices configured to be worn by an individual to apply compression, vibration, and/or heat to cartilage tissue at one or more joints of the individual. The individual dons the therapeutic device so that therapeutic units of the therapeutic device are located in proximity to the cartilage tissue, and electrical power is supplied to a control system and to the therapeutic units to operate and control each of the therapeutic units individually and simultaneously to selectively apply the compression, vibration, and/or heat to the cartilage tissue.

An object of the invention is to provide a cartilage regenerative material that suppresses infiltration of fibrous soft tissue and brings about satisfactory cartilage regeneration, and a method for producing the cartilage regenerative material. Provided is a cartilage regenerative material including a porous body of a biocompatible polymer and a biocompatible polymer film, in which the porous body contains chondrocytes and cartilage matrix, and the cartilage matrix exists in a region of 10% or more of a region extending from the surface of the transplant face of the porous body to a depth of 150 μm along the thickness.

The present invention relates to a method of manufacturing collagen for restoring cartilage tissue usable for a joint in an injection manner, and a usage method thereof. The present invention provides a method of obtaining a high-concentration collagen-solution injection by aseptically filling an injection container with collagen separated from pig skin tissue, and a method of using the high-viscosity collagen loaded into the injection container for the purpose of cartilage restoration. Cartilage tissue regeneration is effectively induced when tissue restoration is implemented with respect to a cartilage-deficient portion using collagen, which is a biocompatible material, in a form that is capable of being injected into an application site with an injection needle without a surgical incision. Accordingly, restoration and regeneration of cartilage are easily and quickly induced in an animal, excluding a human, while relieving the burden related to surgery.

A composite material can include a gel and at least one nanostructure disposed within the gel. A method for healing a soft tissue defect can include applying a composite material to a soft tissue defect, wherein the composite material includes a gel and a nanostructure disposed within the gel. A method for manufacturing a composite material for use in healing soft tissue defects can include providing a gel and disposing nanofibers within the gel.

The disclosure provides a chondrocyte cell sheet comprising one or more layers of confluent cells comprising chondrocytes and chondroprogenitor cells. Methods of generating cartilage tissue in a subject are also provided. The disclosure also provides a method for producing chondrocyte cell sheets comprising culturing chondrocytes and chondroprogenitor cells in culture solution on a temperature-responsive polymer which has been coated onto a substrate surface of a cell culture support, wherein the temperature-responsive polymer has a lower critical solution temperature in water of 0-80° C.; adjusting the temperature of the culture solution to below the lower critical solution temperature, whereby the substrate surface is made hydrophilic and adhesion of the cell sheet to the surface is weakened; and detaching the cell sheet from the culture support.

A meniscus replacement device for replacing damaged soft tissue at a host knee includes a first component comprising a first tissue-interface surface shaped to free-floatingly interface with tissue structure of one of a femur and a tibia in a knee joint having a damaged soft tissue, and comprises a second component comprising a second tissue-interface surface shaped to free-floatingly interface with a second tissue structure of the other of the femur and the tibia in the knee joint. The second component may include a containment cavity receiving at least a portion of the first component. In another form, the free floating soft joint tissue replacement component and the base component are fixed together. In some aspects, the second tissue-interface surface is shaped to fit contours of a natural tibia plateau. In some aspects, the first tissue-interface surface is shaped to fit contours of a femoral surface.

Provided herein are meniscus implant compositions, as well as method for making and using the same. The subject meniscus implants find use in repairing and/or replacing damaged or diseased meniscal tissue in a mammalian subject.

An enhanced bone graft product includes a host matrix, a bone graft material, a scaffold, or a biological construct, and a composition including a therapeutically effective amount of protocatechuic acid. The bone graft material may be autographic, allographic, alloplastic, or xenographic. The bone graft material may include fresh bone, freeze-dried bone, or demineralized freeze-dried bone. The composition may include a pharmaceutically acceptable carrier, adjuvant, or vehicle. The composition may include protocatechuic acid at a concentration of about 10 μM to about 250 μM. The composition may further include mesenchymal stem cells, adipose tissue-derived stem cells, endothelial progenitor cells, mesenchymal skeletal stem cells, synovial stem cells, bone marrow extract, osteoblasts, chondrocytes, osteocytes, or combinations thereof.