The present invention relates to a method of preparing a three-dimensional (3D) cell composition comprising the steps of a) forming a support matrix containing oral fibroblasts suspended within the support matrix by mixing fibrinogen, a modifier and oral fibroblasts with thrombin, b) incubating the support matrix in a cell culture media for a sufficient time to allow development of a first layer of the three-dimensional cell composition, and c) seeding oral keratinocytes on a surface of the first layer and culturing the oral keratinocytes to form a second layer of the three-dimensional cell composition. In specific embodiments of the invention, the method is exemplified for the production of an artificial gingival tissue, wherein polyethylene oxide), 4-arm, succinimidyl glutarate terminated is used as the modifier. Also disclosed are uses of the 3D cell composition which include treatment of a gum disease or condition, regenerative therapy and for in vitro testing.

The present disclosure relates to a method for removing residual acid of implant that has been surface treated using acid, the method including thermal decomposition step of thermally decomposing and removing the acid remaining on the implant; base treatment step of treating the acid remaining on the implant with base, thereby neutralizing and removing the acid; and washing step of washing and removing the acid and the base remaining on the implant with washing water. According to the present disclosure, the acid remaining on the surface of the fixture can be effectively removed, and thus there is an effect of preventing the problem of bone loss that may occur near the placed implant.

In one aspect, the disclosure relates to protective, anti-bacterial coatings for medical implants and methods of making the same. Also disclosed herein are methods for improving the anti-bacterial properties of a medical device coated with silicon carbide (SiC) or titanium nitride (TiN). Further disclosed herein are medical devices including an anti-microbial layer prepared by the disclosed methods. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Provided herein are compositions for bone formation, comprising a scaffold of hydroxyapatite (HA) and tricalcium phosphate (TCP) in a ratio of from 0/100 to 15/85, collagen, and bioactive glass, wherein the bioactive glass is uniformly dispersed in both interior and surface portions of the scaffold, and wherein the composition is sterilized and packaged.

The systems and methods herein are directed to photo-biomodulation (PBM) and anti-microbial photo disinfection therapy, aPDT, a low laser light therapy (LLLT) of morula stem cells in gingivae (GMoSC) in-vivo and in-vitro for autologous and/or allogenic applications in tissue and organ engineering with or without exosomes. The morula stem cells are initially found in gingivae in a clustered state where their proliferation capabilities are preserved through life, suitable for autologous and/or allogenic applications. The morula stem cells in gingivae tissues in the imperceptible suture of maxillae/pre-maxillae in a clustered state as a coating of gingivae tissues have been treated with PBM, aPDT, or LLLT, thereby clustered state is laser excited, which allows the morula stem cells to be imbued with a first proliferation effect and a second proliferation effect.

The present disclosure features adhesive compositions and methods of use thereof related to the medical, veterinary, and dental fields.

The present invention relates to preparation of bioink composed of cellulose nanofibril hydrogel with native or synthetic Calcium containing particles. The concentration of the calcium containing particles can be between 1% and 40% w/v. Such bioink can be 3D Bioprinted with or without human or animal cells. Coaxial needle can be used where cellulose nanofibril hydrogel filled with Calcium particles can be used as shell and another hydrogel based bioink mixed with cells can be used as core or opposite. Such 3D Bioprinted constructs exhibit high porosity due to shear thinning properties of cellulose nanofibrils which provides excellent printing fidelity. They also have excellent mechanical properties and are easily handled as large constructs for patient-specific bone cavities which need to be repaired. The porosity promotes vascularization which is crucial for oxygen and nutrient supply. The porosity also makes it possible for further recruitment of cells which accelerate bone healing process. Calcium containing particles can be isolated from autologous bone, allogenic bone or xenogeneic bone but can be also isolated from minerals or be prepared by synthesis. Preferable Calcium containing particles consist of β-tricalcium phosphate which is resorbable or natural bone powder, preferably of human or porcine origin. The particles described in the present invention have particle size smaller than 400 microns, or more preferably smaller than 200 microns, to make it possible to handle in printing nozzle without clogging and to obtain a good resolution. Cellulose nanofibrils can be produced by bacteria orbe isolated from plants. They can be neutral, charged or oxidized to be biodegradable. The bioink can be additionally supplemented by other biopolymers which provide crosslinking. Such biopolymers can be alginates, chitosans, modified hyaluronic acid or modified collagen derived biopolymers.

The invention provides composite materials that form a biocompatible and bioresorbable settable ceramic-forming composition, and that possesses high strength when set and other desirable mechanical properties. The composite materials may include additive materials that provide beneficial advantages in the handling and physical properties of the material. When a hydrated precursor, the composite material is capable of being injected through cannulas for placement in treatment sites. The composite material provided desirable handling properties and sets in a clinically relevant time period.

A support element (S) for supporting implants (I), such as dental implants, the support element comprising a bar (S1) and a plurality of pins (S5) that are fitted to the bar (S1) and that are arranged parallel to one another, each pin (S5) defining a free end that is provided with reception means (S8) that are suitable for co-operating with the implant (S) so as to hold it on the reception means (S8) of the pin (S5), the bar (S1) including at least one mounting end (S4) for mounting the bar (S1) on another support device, thereby forming a support structure; the support element being characterized in that each pin (S5) is provided with a removal system (S9) for removing the implant (S) from the reception means (S8), without coming into contact with an exposed portion of the implant (S).

The present invention relates to a bone implant with a porous lithium tantalate membrane and a method for preparing the bone implant. The bone implant comprises: (1) a substrate; and (2) a porous membrane on the substrate, wherein the substrate is selected from the group consisting of a tantalum substrate, a niobium substrate, a tantalum-niobium alloy substrate and a titanium substrate, and wherein the porous membrane is selected from the group consisting of a porous lithium tantalate membrane, a porous lithium niobate membrane, a porous lithium tantalate-lithium niobate mixture membrane and a porous titanium oxide membrane. The bone implant of the present invention has one or more of the following beneficial effects: (1) The bone implant has excellent corrosion resistance; (2) the elasticity modulus of the bone implant can be adjusted according to process conditions so that it has higher biocompatibility with the elasticity modulus of a human or animal bone (such as an alveolar bone and a cranium); (3) the white color of the bone implant is close to the color of the bone itself and the bone implant has an aesthetic appearance; (4) the bone implant has excellent bacteriostatic properties.