The invention relates to a device for separation of fluidal biological material components, particularly separation of components of the material collected by liposuction for subsequent transfer of the adipose tissue in an injection form. The device comprises a container with an outlet duct arranged in it, the outlet duct having its upper end adapted for collecting the material, and its lower end that is in fluid communication with the inner space of the container at its bottom. Further, the invention relates to a separating float for separation of the fluidal biological material components, the float being intended to be used in the device, wherein the float comprises at least one by-pass opening for passage of the fluidal biological material contained in the container from the area below the separating float into the area above it. The invention also relates to a kit including the device and the float.

Preparation of a product comprising stromal vascular fraction cells includes washing human biological material comprising adipose in a container apparatus having an internal filter, which divides an internal containment volume of the container apparatus into a tissue retention volume on one side of the filter and a filtrate volume on an opposite side of the filter, and a mixing device with at least one rotatable mixing member disposed in the tissue retention volume. The washing includes operation of the mixing device to rotate the mixing member through the human biological material within the tissue retention volume, and the washing is followed by digesting washed material within the internal containment volume with added enzyme, centrifuging of the container apparatus to prepare a centrifuged pellet in the filtrate volume, selectively removing material of the pellet and preparing a product with a mixture of stromal vascular fraction cells of removed pellet material and an aqueous suspension liquid.

The invention is a process and system, consisting of a reinjection pump, connecting tubing, a reinjection cannula and a recipient site, with fluid management features including a peristaltic pump head and controls for pressure limits, flow rates and flow distribution wherein the process of controlling the reinjected fat and fluid is unique because of the control of flow rates, pressures, matching cannula hole sizes, and maintaining a closed continuous system where the harvesting and reinjection is done all together with a completely closed system. The process utilizes a method and control system to manage pressure levels during reinjection procedures of viable soft tissue. Although this case specifically relates to its use in the reinjection of adipose fat and other tissue back into the body during liposuction procedures, it can be applied to any medical procedure of introducing or re-introducing materials into the body.

The present disclosure relates to a tissue collection apparatus. The tissue collection apparatus comprises a housing defining an inlet and an outlet, a first filter disposed within the housing, a second filter disposed within the housing, the second filter configured to isolate tissue particles of a desired size that pass through the first filter under the application of an aspiration force applied through the housing. A method of harvesting tissue is also disclosed.

A closed system for harvesting fat through liposuction, concentrating the aspirate so obtained, and then re-injecting the concentrated fat into a patient comprises as its main components a low pressure cannula having between about 7 to 12 side holes of about 1-2 mm by 2.0 to 4.0 mm, a spring loaded syringe holder with a constant force or coiled ribbon spring to apply a substantially constant pressure over the full excursion of the plunger, and a preferably flexible collection bag which is also preferably graduated, cylindrical over most of its body and funnel shaped at its bottom, all of which are connected through flexible tubings to a multi-port valve. The multi-port valve has two flutter/duck bill valves which restrict the fluid flow to a one way direction which effectively allows the syringe to be used to pump fat out of a patient and into a collection bag in a continuous manner. After the bags are centrifuged to concentrate the fat, the excess fluids are separated and the valve is re-connected to permit the syringe pump to reverse fluid flow to graft the concentrated fat back into the patient.

A method for ex vivo treating blood or plasma is provided. The method includes (a) ex vivo contacting a blood or plasma with an enzyme composition to react the enzyme composition with the blood or plasma, wherein the enzyme composition is capable of eliminating electronegative low-density lipoprotein from the blood or plasma by the activity of the enzyme composition, and the enzyme composition is selected from a group consisting of: a first enzyme for eliminating a glycan residue of an electronegative low-density lipoprotein (LDL); a second enzyme for eliminating ceramide carried by a electronegative low-density lipoprotein (LDL); and a combination thereof; and (b) terminating contact between the blood or plasma and the enzyme composition to terminate the reaction of the enzyme composition with the blood or plasma.

A system for determining components of matter removed from a body includes a container for receiving the matter, a device for weighing the container and the matter therein, an instrument for obtaining information relating to determine the components of the matter based in part on a weight of the matter determined by said device and the information relating to the at least one aspect of the matter obtained by said instrument. A display includes a screen for displaying information relating to the matter provided by said processer. The information may include a height and/or a color of the matter among other aspects. An insert containing an absorbent material may be utilized to separate fat cells from a fluid when liposuction is used to remove the matter. The insert may be porous allowing fluid to enter the absorption.

The invention is a method and control system to manage pressure levels during reinjection procedures of viable soft tissue. Although this case specifically relates to its use in the reinjection of adipose fat and other tissue back into the body during harvesting and reinjection procedures, it can be applied to any medical procedure of introducing or re-introducing materials into the body.

A medical instrument includes a handpiece, an electromechanical transducer disposed in the handpiece, and an electrical circuit disposed at least partially in the handpiece for supplying alternating electrical current of a predetermined frequency to the transducer. A probe is operatively connected to the transducer for transmitting vibrations generated by the transducer to an operative site in a patient. A switching device is mounted to the handpiece and is operatively connected to the circuit and the transducer for enabling the supply of power to the transducer during a motion of the probe in a preselected direction relative to the handpiece and for disabling the supply of power to the transducer upon a termination of motion of the probe in the preselected direction.

A cell washing apparatus is provided to wash a cell-containing fluid. The apparatus is arranged to exchange one or more exchangeable entities from a cell-containing first fluid, and comprises, a first fluid conduit and a second fluid conduit, the second fluid conduit separated from the first fluid conduit by a semi-permeable membrane disposed therebetween; the first fluid conduit having a first fluid inlet and a first fluid outlet, the first fluid conduit arranged to transport the first fluid in a first direction between the first fluid inlet and the first fluid outlet; the second fluid conduit being arranged to house a second fluid; wherein the semi-permeable membrane comprises a plurality of pores arranged to permit transport of said one or more exchangeable entities from the first fluid to the second fluid; wherein the one or more exchangeable entities comprise free haemoglobin and/or blood plasma; and wherein the first fluid is whole blood isolated from a human body, or packed red blood cells. The cell washing apparatus of the present invention aims to solve the problem of harmful species that accumulate in a cell-containing fluid (such as, for example, transfusion blood) during storage and other applications, in which it is desired to transfer red blood cells to a clean suspending liquid.