The present invention relates to an affinity purification system comprising a polypeptide (protein) that binds selectively (e.g. specifically) and reversibly to its cognate peptide tag (ligand). In particular, the polypeptide comprises: (i) an amino acid sequence as set forth in SEQ ID NO: 1, wherein X at position 77 is selected from alanine, glycine, serine, asparagine, or threonine; (ii) a portion of (i) comprising an amino acid sequence as set forth in SEQ ID NO: 2, wherein X at position 56 is selected from alanine, glycine. serine, asparagine or threonine; (iii) an amino acid sequence with at least 80% sequence identity to a sequence as set forth in SEQ ID NO: 1, wherein the polypeptide comprises alanine, glycine, serine, asparagine or threonine at a position equivalent to position 77 of SEQ ID NO: 1. and wherein the poly peptide comprises histidine at positions equivalent to positions 30, 84 and 85 of SEQ ID NO: 1; or (iv) a portion of (iii) comprising an amino acid sequence with at least 80% sequence identity to a sequence as set forth in SEQ ID NO: 2, wherein the polypeptide comprises alanine, glycine, serine, asparagine or threonine at a position equivalent to position 56 of SEQ ID NO: 2, and wherein the polypeptide comprises histidine at positions equivalent to positions 9, 63 and 64 of SEQ ID NO: 2, wherein the polypeptide binds selectively and reversibly to a peptide comprising an amino acid sequence as set forth in SEQ ID NO: 3, 4 or 5.

The present invention relates to an affinity purification system comprising a polypeptide (protein) that binds selectively (e.g. specifically) and reversibly to its cognate peptide tag (ligand). In particular, the polypeptide comprises: (i) an amino acid sequence as set forth in SEQ ID NO: 1, wherein X at position 77 is selected from alanine, glycine, serine, asparagine, or threonine; (ii) a portion of (i) comprising an amino acid sequence as set forth in SEQ ID NO: 2, wherein X at position 56 is selected from alanine, glycine. serine, asparagine or threonine; (iii) an amino acid sequence with at least 80% sequence identity to a sequence as set forth in SEQ ID NO: 1, wherein the polypeptide comprises alanine, glycine, serine, asparagine or threonine at a position equivalent to position 77 of SEQ ID NO: 1. and wherein the poly peptide comprises histidine at positions equivalent to positions 30, 84 and 85 of SEQ ID NO: 1; or (iv) a portion of (iii) comprising an amino acid sequence with at least 80% sequence identity to a sequence as set forth in SEQ ID NO: 2, wherein the polypeptide comprises alanine, glycine, serine, asparagine or threonine at a position equivalent to position 56 of SEQ ID NO: 2, and wherein the polypeptide comprises histidine at positions equivalent to positions 9, 63 and 64 of SEQ ID NO: 2, wherein the polypeptide binds selectively and reversibly to a peptide comprising an amino acid sequence as set forth in SEQ ID NO: 3, 4 or 5.

To provide a chromatography carrier that has excellent antifouling properties and exhibits excellent pressure-resistant performance and shatter-resistant performance. A chromatography carrier comprising: a polymer having a partial structure containing at least two groups represented by C(O)NH.

Anion exchange-hydrophobic mixed mode ligands and methods of their use are provided.

Anion exchange-hydrophobic mixed mode ligands and methods of their use are provided.

The present invention relates to superficially porous particles (SPPs), also called core-shell, porous shell or fused core particles, which are state-of-the-art support materials used in the production of HPLC columns. Hydrolytically stable, highly selective superficially porous particle (SPP) hydrophilic interaction liquid chromatographic (HILIC) stationary phases having higher efficiencies and shorter retention times than analogous stationary phases on fully porous particles (FPP) is provided.

The present invention relates to superficially porous particles (SPPs), also called core-shell, porous shell or fused core particles, which are state-of-the-art support materials used in the production of HPLC columns. Hydrolytically stable, highly selective superficially porous particle (SPP) hydrophilic interaction liquid chromatographic (HILIC) stationary phases having higher efficiencies and shorter retention times than analogous stationary phases on fully porous particles (FPP) is provided.

A superpolar sorbent network is a sol-gel network of at least one metal oxide precursor condensed and at least one polyhydroxy molecule. The metal oxide precursor is a silicate precursor, aluminate precursor, titanate precursor, zirconate precursor, germinate precursor, or any combinations thereof, and the polyhydroxy molecule has a multiplicity of hydroxyl groups. The polyhydroxy molecule can be an organic molecule derived from nature. The superpolar sorbent network can be used as a particulate or bulk sorbent for sampling or removal of analytes or contaminants from an environment or can be coated on a tube or particulate substrate for use as a chromatographic stationary phase.

A superpolar sorbent network is a sol-gel network of at least one metal oxide precursor condensed and at least one polyhydroxy molecule. The metal oxide precursor is a silicate precursor, aluminate precursor, titanate precursor, zirconate precursor, germinate precursor, or any combinations thereof, and the polyhydroxy molecule has a multiplicity of hydroxyl groups. The polyhydroxy molecule can be an organic molecule derived from nature. The superpolar sorbent network can be used as a particulate or bulk sorbent for sampling or removal of analytes or contaminants from an environment or can be coated on a tube or particulate substrate for use as a chromatographic stationary phase.

The present invention provides a lipid extraction device comprising a capillary tube, wherein the capillary tube comprises a first region containing a first filler; and a second region present in a region other than the first region and containing a second filler having polarity different from the first filler. The present invention also provides a system and a method for extracting and analyzing lipids from a biological sample using the lipid extraction device.