Methods of making functionalized support material are disclosed. Functionalized support material suitable for use in chromatography columns or cartridges, such as in a high pressure liquid chromatography (HPLC) column or a fast protein liquid chromatography (FPLC) column, is also disclosed. Chromatography columns or cartridges containing the functionalized support material, and methods of using functionalized support material, such as a media (e.g., chromatographic material) in a chromatography column or cartridge, are also disclosed.

Methods of making functionalized support material are disclosed. Functionalized support material suitable for use in chromatography columns or cartridges, such as in a high pressure liquid chromatography (HPLC) column or a fast protein liquid chromatography (FPLC) column, is also disclosed. Chromatography columns or cartridges containing the functionalized support material, and methods of using functionalized support material, such as a media (e.g., chromatographic material) in a chromatography column or cartridge, are also disclosed.

The present invention provides a stationary phase for solid-phase microextraction (SPME) devices based on nickel and titanium alloy nuclei and a metal-organic framework (MOF) exterior, which may be used for chromatographic analysis in environmental, food, etc. applications. The method of preparation of the stationary phases consists of a number of steps which provide a covalent adhesion of the MOF to the nickel/titanium alloy. In these stationary phases, the metal-organic framework is the only component that comes into contact with the sample to be analysed. The interior of the stationary phase is executed in nitinol and endows the system with thermal and mechanical stability, this being the first time that it is used to support a metal-organic framework, and presenting extractive advantages in comparison with commercial SPME stationary phases.

A separating medium for hydrophilic interaction chromatography useful in separating hydrophilic compounds. The hydrophilic interaction chromatography separating medium, which is formed from a support and a ligand carried by the support, is a separating medium wherein the ligand is a (meth)acrylic polymer having a constituent unit derived from the compound indicated by formula (I). ##STR00001##

A separating medium for hydrophilic interaction chromatography useful in separating hydrophilic compounds. The hydrophilic interaction chromatography separating medium, which is formed from a support and a ligand carried by the support, is a separating medium wherein the ligand is a (meth)acrylic polymer having a constituent unit derived from the compound indicated by formula (I). ##STR00001##

The invention relates to a novel biomimetic affinity purification material and its application in the purification of chitosanase, which belongs to the field of industrial biotechnology. The affinity ligand for the biomimetic affinity material is chitodisaccharides, the connecting arm is cyanuric chloride, and the base medium is epoxy-activated Sepharose™ 6B. The desorption constant (Kd) and the theoretical maximum adsorption capacity (Qmax) of the biomimetic affinity material are 24.2 μg/mL and 24.1 mg/g, respectively. Using the above biomimetic affinity material, a chitosanase biomimetic affinity purification method is established, which can produce high-purity chitosanase with high efficiency and low cost, and has good industrial application potential.

The invention relates to a novel biomimetic affinity purification material and its application in the purification of chitosanase, which belongs to the field of industrial biotechnology. The affinity ligand for the biomimetic affinity material is chitodisaccharides, the connecting arm is cyanuric chloride, and the base medium is epoxy-activated Sepharose™ 6B. The desorption constant (Kd) and the theoretical maximum adsorption capacity (Qmax) of the biomimetic affinity material are 24.2 μg/mL and 24.1 mg/g, respectively. Using the above biomimetic affinity material, a chitosanase biomimetic affinity purification method is established, which can produce high-purity chitosanase with high efficiency and low cost, and has good industrial application potential.

The present disclosure is directed to stationary phase materials for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified silica particle stationary phase materials, which are optionally also methoxy-terminated polyethylene glycol surface modified.

The present disclosure is directed to stationary phase materials (e.g., porous inorganic-organic hybrid particles) for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified stationary phase materials.

The present disclosure is directed to stationary phase materials (e.g., porous inorganic-organic hybrid particles) for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified stationary phase materials.