The inventions cover systems and methods for generation of emulsions having suitable clarity without requiring refractive index matching between emulsion components. Systems can include: a substrate including a set of openings; a reservoir facing the substrate at a first side and containing a sample fluid configured for droplet formation upon interacting with the set of openings of the substrate; and a collecting container facing the substrate at a second side and containing a set of fluid layers configured with a density gradient and suitable immiscibility characteristics. One or more components of the system(s) can support methods for emulsion generation, in relation to enabling interactions between multiple continuous phases and a dispersed droplet phase to generate clear emulsions. Applications of the inventions(s) can include performance of droplet-based digital PCR in an improved manner (e.g., without requiring implementation of correction factors based upon Poisson statistics).

A device of immobilising and concentrating cells from cell suspensions by centrifugation using a standard centrifuge having a plurality of centrifuge tubes having respective closing lids, the device comprising: one or more funnel units, each having a broad side and a narrow side, adapted to receive one or more fluids samples; one or more hollow cylinder, having a plurality of external threads on lateral surface of the cylinder, attached at the narrow side of the each of the respective one or more funnel unit, enclosing the narrow side of the respective one or more funnel unit; one or more removable gasket disposed on the narrow side encircling circumference of the narrow side of each of the one or more funnel unit; one or more coverslip holding plate, adapted to hold a respective coverslip, deployed proximal to the circumference of the narrow side of the respective one or more funnel unit; and one or more removable caps having a plurality of internal thread, adapted to enclose the respective one or more coverslip holding plates along with the respective coverslip, configured to fasten over the respective one or more hollow cylinder; wherein the one or more removable gasket is adapted to restrict leaking of the one or more fluid samples inside the one or more funnel units.

The present disclosure provides an anti-pollution consumable and a method for Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) molecular diagnosis using the same, belonging to the technical field of nucleic acid detection and molecular diagnostics. The anti-pollution consumable includes an outer reaction tube, a sleeve and an inner reaction tube, where the inner reaction tube includes a second hollow cylindrical upper body and a second-type conical lower body sequentially from top to bottom; a top end of the second hollow cylindrical upper body is externally connected with a fixing ring perpendicular to the second hollow cylindrical upper body; a number of drain holes are provided at a bottom of the second-type conical lower body; the drain hole has a diameter of 0.01-0.8 mm; the drain hole is used for hydrophobic treatment; and the inner reaction tube is fixed inside the outer reaction tube through the sleeve.

Disclosed is a system to separate, enrich, and/or purify a cellular population from a biological tissue, such as a tissue sample. For example, an adipose tissue sample can be acquired and disrupted. The disrupted tissue sample can then be separated and purified. The separated components can include multipotent, pluripotent, or other cell populations.

Methods and devices are provided for pre-treatment of a sample containing microbial cells. In some embodiments, the pre-treatment of the sample is performed via the initial selective lysis, within a sample pre-treatment vessel, of non-microbial cells, such as blood cells, and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pre-treatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.

The present specification discloses filtration devices for processing a biofluid sample, methods of purifying a biofluid sample using the disclosed filtration devices, and systems comprising components of the filtration devices. The filtration device generally includes a collection container, a quantitative container, a filter, and a plunger slidably inserted through a plunger attachment. In a first configuration, the quantitative container is axially slidably inserted within the collection chamber, with fluid communication between the quantitative chamber and the collection chamber provided through the filter. In a second configuration, the filter and the collection container are separated from the quantitative container, the plunger attachment is fitted to the quantitative chamber open end with at least a portion of the plunger positioned within the quantitative chamber.

Provided are a medical analysis device and a cell analysis method which can capture many cancer cells, including cancer cells not expressing EpCAM. A medical analysis device including a flow channel zone that includes a chamber zone, the inner surface of the flow channel zone being at least partially provided with a layer of a hydrophilic polymer having a thickness of 2 to 200 nm.

A system for separating and concentrating a component of a whole material is disclosed. The whole material can include a material that has more than one component, such as whole blood that can include at least red blood cells, monocytes, and plasma. The system can include a substantially single container including a separation section and a concentration section wherein a component can be moved from the separation section, after a separation, to the concentration section to be concentrated. The concentrated component can then be withdrawn from the separation and concentration container for a selected procedure.

Kits and methods provide for the isolation of white blood cells from bodily fluids. In one exemplary aspect, a method for isolating white blood cells from blood includes the act of adding a blood sample to a separation tube having a distal end, a proximal end, and a valve located proximate said proximal end, said valve being configured to transition between at least first, second, and third positions. The method also includes the act of removably attaching a cap to the distal end, centrifuging the separation tube with the valve in the first position, removing the cap at the distal end of the separation tube and removably attaching a first syringe to the proximal end, switching the valve to the second position and withdrawing, via the first syringe, a red blood cell sediment. The method also includes the act of switching the valve to the first position and removing the first syringe, adding a small volume of buffer to the separation tube, removably attaching a cap to the distal end and centrifuging the separation tube and removing the cap at the distal end of the separation tube and removably attaching a second syringe to the proximal end. Additional acts include switching the valve to the second position, withdrawing the remaining red blood cell sediment via the second syringe and switching the valve to the first position and removing the second syringe.

A system for preparing a serum includes a containment device, a cage positioned inside the containment device, a cap attachable to the containment device and configured to cover the cage, an inlet port configured to introduce an autologous fluid into the containment device, and an outlet port configured to remove a serum from the containment device.