The present invention relates to microfluidic fluidic devices, methods and systems as microfluidic kidney on-chips, e.g. human Proximal Tubule-Chip.

An embodiment of a channel device (1) according to the present disclosure includes a channel (2) and a first space (3) and a second space (4) located in the channel (2). The channel (2) includes a side surface along a direction in which a liquid flows. The second space (4) is connected to the first space (3). An upper end of the second space (4) is located at a different height from an upper end of the first space (3). At least a part of the first space (3) is located between the side surface of the channel (2) and at least a part of an outer periphery of the second space (4).

A method for detecting an analyte reactive towards luminol, comprising the steps of: feeding into a reaction chamber an alkaline solution of luminol, noble metal nanoparticles and at least one analyte reactive towards luminol, wherein the reaction chamber is in the form of a curved channel; detecting the light emitted due to a chemiluminescence reaction taking place in said channel; and discharging a reaction mass from said channel, characterized in that the average diameter of the metal nanoparticles is greater than 25 nm. Also provided is a microfluidic device for carrying out the method.

Example implementations include a method of manufacturing a biochemical sensor by forming a fluid conduit in a microfluidic layer, forming an electrode on an electrode layer, forming a biochemical sensor on the electrode layer, bonding the electrode layer to a first surface of the microfluidic layer, and bonding a barrier layer to a second surface of the microfluidic layer. Example implementations also include a method of electrically detecting a biochemical by contacting an electrode array to a biological surface, obtaining a biofluid at the electrode array from the biological surface, obtaining a response current associated with the biofluid at the electrode array, and generating a quantitative biochemical response based at least partially on the response current. Example implementations further include applying a current to the biological surface. Example implementations further include filtering electrical interference at the electrode array. Example implementations further include generating a quantitative biochemical response based on the response current.

Embodiments relate to a bioagent capture and identification system including a microfluidic platform for label-free, size-based capture, enrichment, and optical profiling of bioagents using vertically aligned carbon nanotubes coated in gold nanoparticles. Bioagent identification can be automated using machine learning. Captured bioagents remain viable after capture and analysis. In the nanotube fabrication process, catalyst precursor layers are fabricated using patterned stamps. In addition, nanotube diameter and density are increased by increasing the concentration of metal content in the catalyst precursor layer.

Provided is a digital microfluidic device for quick polymerase chain reaction. The digital microfluidic device includes an enclosed chamber for holding droplets comprising PCR mixtures. The chamber has an upper layer and a lower layer, which provide a top heater and a bottom heater contained in a thermal electrode respectively to form dual heaters. The lower layer further has an array of electrodes and a dielectric layer, e.g. Norland Optical adhesive 61, coating thereon. Such arrangement of the digital microfluidic device allows quick and homogeneous heating of droplets to lower the heating voltage, shorten the reaction time, and prevent the dielectric layer from breakdown during the thermal cycle.

A system for transferring a sample into a microfluidic system, including a sample loading chamber, wherein a first sub-volume of the sample loading chamber is separated from at least one second sub-volume of the sample loading chamber by a filter module. The first sub-volume forms a pressure chamber provided for the loading of the sample, and there is at least one second sub-volume for providing the microfluidic system with the sample.

The application relates to a sample holder (110) and a system (100). The application also relates to a method for processing a biological sample (S) and use of the sample holder or of the system in an analytical method or a diagnostic method. The sample holder (110) comprises a tubular member (111) with a wall that is at least locally transparent and at least locally permeable for reagents, wherein the tubular member consists at least partially of a transparent material.

The present invention relates to a method for determining an analyte using surface enhanced RAMAN spectroscopy and to a device which is suitable for this purpose.

The present disclosure relates to a biological test cassette and a medical test system, the biological test cassette comprising a support, a cassette body, a chip, a first connecting tube and a second connecting tube. The support is provided with a first via hole. The cassette body is provided with a second via hole corresponding to the position of the first via hole, mounted on a top surface of the support, and further provided with a pretreatment chamber and a first flow channel in communication with the pretreatment chamber. The chip is slidably provided on a bottom surface of the support, and provided with an analyzing and processing chamber and a second flow channel in communication with the analyzing and processing chamber. The second connecting tube can be freely bent and deformed during the process of pulling the chip out from or pushing the chip back into the support.