The present disclosure relates to methods of producing viable Cannabis sativa seeds by inducing male flowers on a genetically female Cannabis sativa plant. The pollen produced by the induced flowers of such plant can be used to pollinate female flowers from the same plant or another female plant to produce seed. In certain embodiments, feminised Cannabis sativa seed is produced on a production scale.

The invention provides novel compositions and methods for liquid-mediated delivery of pollen to a female reproductive part of a recipient plant. The pollen suspension solutions provided include a surfactant, an oil, a solute, or an aqueous solution, and about 2% to about 20% pollen by weight. The methods provided include producing a liquid pollen suspension solution comprising pollen from a donor plant and spraying the solution onto at least a first female reproductive part of a recipient plant, thereby pollinating the female reproductive part with the pollen from the donor plant.

The invention provides novel methods for liquid-mediated delivery of pollen grains to enclosed stigmas in recipient female flowers. For example, methods for liquid-mediated pollination are provided. The methods provided include collecting pollen from a donor plant, suspending the collected pollen in a liquid solution, and introducing said solution to an enclosed stigma of a recipient flower bud on a recipient plant, thereby pollinating the flower with the pollen from the donor plant.

A method of weed control is provided. The method comprises artificially pollinating at least one weed species of interest with pollen that reduces fitness of said at least one weed species of interest, the pollen being in a formulation comprising an effective amount of a carbohydrate. Also provided are compositions and kits that can be used for performing the methods described herein.

A method of applying a Chemical Hybridization Agent (CHA) to a plant. The method includes providing a CHA source and bringing the CHA source in contact with a root of the plant. The method further includes having the root take up a part of the CHA. The disclosure further relates to methods of producing a haploid and/or doubled-haploid plant or hybrid seed. The disclosed method may be used in various aspects of plant breeding.

A breeding method for rapidly stabilizing cotton distant hybridization progeny is disclosed. It belongs to the technical field of plant breeding. The disclosure is based on VSG (G. barbadense semi-compatible material) as female parent, general G. hirsutum materials as male parent of the cross to get F1 generation of G. barbadense-G. hirsutum hybrid materials, and treats the F1 generation of young bolls with 0.03-0.05% of colchicine aqueous solution to induce F2 generation to separate the stable intermediate materials or new varieties with excellent genes of both G. barbadense and G. hirsutum, so as to produce new cotton germplasm resources. The main innovation of the present disclosure lies in that it changes the previous treatment position and method of colchicine on cotton plant, and the disclosure is not limited by haploid and directly treats the cotton bolls on F1 cotton plants.

A maize pollen composition containing polyether ether ketone and pollen for collecting pollen and storing pollen.

A method of applying a Chemical Hybridization Agent (CHA) to a plant. The method includes providing a CHA source and bringing the CHA source in contact with a root of the plant. The method further includes having the root take up a part of the CHA. The disclosure further relates to methods of producing a haploid and/or doubled-haploid plant or hybrid seed. The disclosed method may be used in various aspects of plant breeding.

Disclosed are a leaf vein absorption method and a functional modification material for plant. The functional modification material has a covering with a functional material and a protector with a polymeric stabilizing material. The functional material is a unitary, a multivariate, or a high-entropy compound, or a semiconducting or an optoelectronic material. The covering covers a surface of a plant organ, wherein the covering comprises a stomata expansion material to enable the functional material to be absorbed into a plant through stomata. The protector covers a surface of the covering. The functional material is capable of maintaining its functional presence on a surface of the plant until the plant organ undergoes natural apoptosis. Through an absorption technology of leaf veins on the surface of the plant, special functions can be achieved without modifying or implanting plant genes.

The present invention provides pollen compositions, methods and kits for making said compositions, and methods of using said compositions to fertilize a plant. The pollen within these compositions has increased longevity and can be used to fertilize plants for an extended period of time as compared to pollen prepared using conventional methods. Also provided are methods of collecting pollen from a maize plant by maturing individual tassel branches off of the plant and vessels for collecting the pollen from these individual branches.