Patent classifications
A01H1/045
Generation of hapoloid plants based on KNL2
The present invention relates to non-transgenic and transgenic plants, preferably crop plants, comprising at least one mutation of the KINTEOCHORE NULL2 (KNL2) protein, especially a mutation causing a substitution of an amino acid within the KNL2 protein, preferably within the C-terminal region of the KNL2 protein, which preferably have the biological activity of a haploid inducer. Further, the present invention provides methods of generating the plants of the present invention and haploid and double haploid plants obtainable by crossing the plants of the present invention with wildtype plants as well as methods of facilitating cytoplasm exchange.
Red-yellow striped peppers
Certain aspects of the present disclosure relate to chimeric pepper plants that produce pepper fruit having a yellow and red striped color, and to seeds that produce the chimeric pepper plants. Other aspects of the present disclosure relate to methods of generating and selecting chimeric pepper plants that produce pepper fruit having a yellow and red striped color, as well as methods of limiting propagation of off-type pepper plant progeny that produce pepper fruit of a single color.
Powdery mildew resistant pepper plants
The present disclosure provides Capsicum annuum plants exhibiting increased resistance to Leveillula taurica. Such plants comprise novel introgressed genomic regions associated with disease resistance on chromosome 6. In certain aspects, compositions and methods for producing, breeding, identifying, and selecting plants or germplasm with an increased disease resistance phenotype are provided.
Methods for genotyping haploid embryos
The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.
METHOD FOR SELECTING SEED SIZE IN WATERMELON AND GENERATING MODIFICATIONS IN THE TOMATO-SEED SIZE GENE
The disclosure provides methods for selecting watermelon plants for genetic elements on chromosome 2 and 6 which genetic elements predict the average seed size produced in the fruits of said plants and watermelon plants and plant parts comprising modifications in the tomato-seed size gene (Ts-gene) on chromosome 2, and methods of producing seeds and plants.
METHOD FOR IDENTIFYING M1 GENERATION PLANT MUTANTS RESULTING FROM PHYSICAL AND CHEMICAL MUTAGENESIS AND FOR ACQUIRING MUTANT, IDENTIFICATION OF GENOTYPING PRIMER FOR ORYZA SATIVA MUTATION, MUTANT GENE, AND USE THEREOF
The present disclosure discloses a method for identifying a M1 generation plant mutant resulting from physical and chemical mutagenesis, a method for acquiring the plant mutant, a mutant gene, and use thereof. The method for identifying a M1 generation plant mutant resulting from physical and chemical mutagenesis includes: mutagenizing a plant to obtain an M1 generation plant mutant, extracting a mixed pool of DNA from the obtained M1 generation plant mutant, subjecting the mixed pool of DNA to high-depth targeted sequencing for a target gene region, and aligning a sequencing result with the target gene region to identify whether there are target single nucleotide polymorphisms (SNPs) and/or Indel. The method of the present disclosure has high efficiency and high accuracy, involves simple operations, and is of progressive significance for the identification and acquisition of innovative germplasms.
Rice event GSX2-55
The present disclosure provides a rice event GSX2-55 and a detecting and using method thereof, and provides a plant, a plant cell, a tissue or a seed thereof containing a specificity polynucleotide of the event GSX2-55. The present disclosure further provides a measuring method and kit for detecting existence of the rice event GSX2-55 based on DNA sequence of the recombinant construct inserted into rice genome and Flanking sequence of insertion site and a method for generating rice recessive cell nucleus male sterile line.
MOLECULAR BREEDING METHOD FOR WHEAT FUSARIUM HEAD BLIGHT-RESISTANCE
Disclosed is a molecular marker polymerization wheat breeding method for Fusarium head blight-resistance. The method uses an intermediate material for Fusarium head blight-resistance as a resistance source, and excellent varieties (strains) in a northern part of Huanghuai wheat production area as agronomic parents, and uses molecular marker-assisted selection and conventional breeding techniques. The molecular markers are a linked marker LJJT-1 of a Fusarium head blight-resistant gene Fhb1 and linked markers LJJ-2 and LJJ-3 of a Fusarium head blight-resistant gene Fhb2, so as to create a semi-winter Fusarium head blight-resistant breeding material with outstanding target traits and excellent comprehensive traits, which lays a material foundation for cultivating Fusarium head blight-resistant wheat varieties suitable for planting in the Huanghuai wheat production area.
GENETIC LOCI ASSOCIATED WITH DISEASE RESISTANCE IN SOYBEANS
The present invention relates to methods and compositions for identifying, selecting and/or producing a Disease resistant soybean plant or germplasm using markers, genes and chromosomal intervals derived from Glycine clandestina, e.g. PI339656, or a progeny thereof. A soybean plant or germplasm that has been identified, selected and/or produced by any of the methods of the present invention is also provided. Disease resistant soybean seeds, plants and germplasms are also provided.
Stevia Cultivar '320032' With Super High Rebaudioside A Content
A stevia cultivar with super high Rebaudioside A content, designated ‘320032’, is disclosed. Another embodiment relates to the plant parts of stevia cultivar ‘320032’, to the plants of stevia ‘320032’ and to methods for producing a stevia plant produced by crossing the cultivar ‘320032’ with itself or another stevia variety, including methods using marker assisted breeding. Another embodiment further relates to hybrid stevia seeds and plants produced by crossing the cultivar ‘320032’ with another stevia cultivar. Twelve highly polymorphic SNPs loci and the corresponding genomic sequences used to identify plant variety ‘320032’-derived plant materials are also disclosed.