A target component is collected using a preparative separation and purification device having a holder for holding a trap column in which the target component has been captured, a liquid feeder for feeding a first solvent having compatibility with the water remaining in the trap column and a second solvent having low compatibility with water and high compatibility with the first solvent into the trap column, a flow-path switch for connecting the exit end of the trap column to a waste liquid flow path and a collection flow path, and a control unit for controlling the flow-path switch so that solution including water flows into the waste liquid flow path.

When a liquid in the column is replaced by carbon dioxide in a supercritical state in the chromatograph, an operation of a first pump is controlled by a flow rate control unit, and the carbon dioxide in the supercritical state is supplied at a constant pressure. Moreover, when a flow rate of the carbon dioxide in the supercritical state reaches a predetermined flow rate thereafter, the flow rate control unit controls an operation of the first pump so that the carbon dioxide in the supercritical state is supplied at a constant flow rate.

Exemplary embodiments of the present disclosure are directed to manipulating pressure-related hysteresis in a pressurized flow system by setting the pressure of the system to a predetermined location in the hysteresis band to advantageously minimize an effect of the pressure related hysteresis on the pressure of the system or to advantageously benefit from the effects of the hysteresis on the pressure of the system.

The present disclosure generally relates to systems, methods and devices for providing pressurized solvent flow in chromatography systems.

Systems, methods and devices are taught for providing analytical methods for peak-shaped responses separated in time or space, including quantitation of chromatographic peaks based on a width measurement of a peak trace at a selected height as a quantitation element. Methods of treating a peak trace as a composition of exponential functions representing a leading and a trailing end are included. Methods that facilitate the detection of impurities in peak trace outputs are also included.

A liquid chromatography having an on-line cleaning function, comprising a first flow channel (L3), a second flow channel (L21), an analysis flow channel (L22) and waste liquid flow channels, further comprising a cleaning flow channel (L25), a direction switch valve (V1) and a multi-flow channel switch valve (V2), etc. The liquid chromatography changes the flow path of the liquid by changing the communication relationship between the two-position switch valves, thus realizes the on-line cleaning function for a first chromatographic column (C1), a middle chromatographic column (C2), a filter or a protector (B2) respectively, and realize the simultaneous on-line cleaning function for the first chromatographic column (C1) and the middle chromatographic column (C2).

Examples of gas liquid separators include a chamber, a fluid mixture inlet, a gas outlet and a liquid outlet. The fluid mixture inlet and the gas and liquid outlets are in fluid communication with the chamber. A fluid mixture received at the fluid mixture inlet diffuses inside the chamber and is separated into a liquid and a gas. The separated liquid is gravity-fed to the liquid outlet. The gas liquid separators have reduced dispersion and increased liquid recovery in comparison to conventional gas liquid separators used for chromatographic separations. The reduced dispersion yields an improvement in the shape of chromatographic peaks.

Exemplary embodiments are directed to a gas liquid separator that includes a chamber, a fluid mixture inlet, a solvent outlet and a gas outlet. The gas liquid separator can include a phase-change inducing mechanism disposed in or proximate to the fluid mixture inlet. Exemplary methods of improving separation of a fluid mixture in a gas liquid separator and CO.sub.2-based chromatography flow systems including a gas liquid separator are also provided.

Flow through pressure sensors for use in fluid chromatography systems include a planar device formed from diffusion bonding of a plurality of metallic sheets and at least one sensing element. The planar device has a top surface, a bottom surface and a flow through channel. A diaphragm formed from a portion of one of the top or bottom surfaces is located adjacent to a sensing region of the flow through channel and is attached to the sensing element. The diaphragm is sized to deflect a distance in response to fluid pressure in the sensing region, which has an internal volume of less than about 25 microliters. The diaphragm and attached sensing element form a pressure sensor that measures strain or deflection of the diaphragm to calculate a pressure within the sensing region.

Exemplary embodiments of the present disclosure are directed to manipulating pressure-related hysteresis in a pressurized flow system by setting the pressure of the system to a predetermined location in the hysteresis band to advantageously minimize an effect of the pressure related hysteresis on the pressure of the system or to advantageously benefit from the effects of the hysteresis on the pressure of the system.