A tunable inertial sheathing (TIS) system and methods for particle-size-insensitive high-throughput single-stream focusing of particles suspended in a particle-carrying fluid are provided. The TIS conditions particles to distribute locally within one of compartments of inertial force field, followed by an inertial focusing to migrate it to a single foci. For the particle localization, the TIS system introduces an arbitrary form of peripheral sheathing by generating and accumulating sheath fluid from particle-carrying fluid through a combination of inertial focusing, channel bifurcation and channel confluence. Multiple forms of the TIS system are also provided, each including one main channel and at least one bypass channel. The main channel includes and cascades at least three segments, at least one bifurcating junction and at least one confluence junction.

A microfabricated device having at least one gas-entrapping feature formed therein in a configuration that entraps air bubbles upon wetting the feature with a solvent or solution is described. The device includes a sacrificial residue in contact with the gas-entrapping feature, the dissolution of which guides the wetting of the gas-entrapping feature.

The present invention is in the field of microfluidic devices produced with silicon technology wherein at least one 3D microenvironment is present, a method of producing said device using silicon based technology, and a use of said device in various applications, typically a biological cell experiment, such as a cell or organ on a chip experiment, and use o the device as a microreactor.

A nano-fluidic device includes: a first substrate that has a nanoscale groove on one surface; and a second substrate that is integrally provided with the first substrate by bonding one surface of the second substrate to the one surface of the first substrate and forms a nanochannel with the groove of the first substrate, in which either the first substrate or the second substrate includes at least a thin portion in a part of a position overlapping the nanochannel in plan view, and the thin portion is deformed by pressing to open and close the nanochannel.

A circuit with electrical interconnect for external electronic connection and sensor(s) on a die are combined with a laminated manifold to deliver a liquid reagent over an active surface of the sensor(s). The laminated manifold includes fluidic channel(s), an interface between the die and the fluidic channel(s) being sealed. Also disclosed is a method, the method including assembling a laminated manifold including fluidic channel(s), attaching sensor(s) on a die to a circuit, the circuit including an electrical interconnect, and attaching a planarization layer to the circuit, the planarization layer including a cut out for the die. The method further includes placing sealing adhesive at sides of the die, attaching the laminated manifold to the circuit, and sealing an interface between the die and fluidic channel(s).

A microfluidic device is useful for modelling drug transmission across the vasculature and vascular barriers. The device includes a frame, a fluid-permeable lumen configured to carry a fluid through the frame in a first direction, a first chamber surrounding the lumen, and a second chamber surrounding the first fluid-permeable chamber. At least one surface of the first chamber is configured for deposition of a first population of endothelial cells. An outer surface of the second chamber is configured for deposition a second population of cells. The second chamber is configured to carry a fluid through the frame in a second direction. The fluid-permeable lumen is configured to allow the fluid to permeate through a wall of the lumen into the first chamber, and the first chamber and the second chamber are in fluid communication with each other.

A DNA sequencing device, and related methods, include a nanopore or nanochannel structure, and a nanoelectrode. The nanoelectrode includes electrode members having free ends exposed within the nanopore or nanochannel structure, an electrode gap defined between of the free ends, and plated portions formed on the free ends to provide a reduced sized for the electrode gap.

Described herein is a microfluidic cartridge for purifying target particles or target cells of a predetermined size from contaminants in a sample, the cartridge comprising a first and a second planar support the first and second planar support each having a top surface and a bottom surface, wherein the top surface of the first and/or second planar support comprises at least one embedded channel extending from one or more inlets to one or more outlets; the at least one embedded channel comprising a plurality of obstacles, wherein the microfluidic cartridge comprises at least one void space configured to be deformed when assembling the first and second planar supports into the microfluidic cartridge.

Provided herein are methods for inkjet printing objects, including objects which may be used as elements of microfluidic devices. The microfluidic devices incorporating the elements are also provided. Such microfluidic devices include those configured to quantify the expression and activity of exosomal matrix metalloprotease, MMP14. These microfluidic devices may be used in methods of monitoring breast cancer in patients having breast cancer.

There is disclosed a nanopore support structure comprising a wall layer comprising walls defining a plurality of wells, and overhangs extending from the walls across each of the wells, the overhang defining an aperture configured to support a membrane suitable for insertion of a nanopore. There is further disclosed a nanopore sensing device comprising a nanopore support structure, and methods of manufacturing the nanopore support structure and the nanopore sensing device.