A nucleic acid detection host includes a host body, a detection kit installation area, a sample heating area, a sampling area, and an image collection unit. The detection kit installation area is configured to detachably install a nucleic acid detection kit. The sampling area is disposed above the detection kit installation area and is connected to the detection kit installation area. The sampling area is configured to add a detection solution into the nucleic acid detection kit. The image collection unit is configured to collect an image of the nucleic acid detection kit. A nucleic acid detection device including the nucleic acid detection host is also disclosed. The nucleic acid detection device has a simple structure, which is portable, flexible, and convenient, and can be used at home.

The present disclosure provides systems, methods, and devices for processing a biological sample. The device may be a microfluidic device comprising a first channel, at least one chamber, and a second channel. The first channel may be in fluid communication with the chamber. The chamber may be configured to receive a portion of the biological sample from the channel. The second channel may be configured for pressurized outgassing of the chamber, first channel, or both the chamber and first channel.

A sample treatment and molecule analysis cartridge is configured to be mounted in a treatment machine vertically. The cartridge has a sample inlet opening, a fluidic inlet, and a fluidic outlet. The cartridge houses an extraction chamber extending vertically from the sample inlet opening and connected to the fluidic inlet; a waste chamber extending vertically, alongside the extraction chamber; and a collector extending along the extraction chamber and the waste chamber and having a smaller height than the extraction chamber and the waste chamber. A fluidic circuit connects together the extraction chamber, the waste chamber, the collector, the fluidic inlet, and the fluidic outlet, and is configured to connect the fluidic outlet to vent openings of the extraction chamber, the waste chamber, and the collector, and to connect the bottom end of the extraction chamber to the fluidic inlet, the waste chamber, and the collector.

A disposable assay platform for detecting a target nucleic acid comprising multiple chambers and a method for operating the assay platform. Solutions containing the target nucleic acid move from one chamber to the next chamber by opening a vent pocket. The resulting pressure change enables the solution to flow to the next chamber. The platform comprises an electronic layer and one or more fluid layers bonded together. All heating operations can be performed by using resistive heating elements in the platform. All cooling operations are preferably passive. The platform is preferably operated when in a vertical orientation and can be docked to an external docking station that controls the operation of the platform.

The technology described herein generally relates to microfluidic cartridges configured to amplify and detect polynucleotides extracted from multiple biological samples in parallel. The technology includes a microfluidic substrate, comprising: a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another: an inlet; a first valve and a second valve; a first channel leading from the inlet, via the first valve, to a reaction chamber; and a second channel leading from the reaction chamber, via the second valve, to a vent.

The present application is generally directed to systems, methods, and devices for diagnostics for sensing and/or identifying pathogens, genomic materials, proteins, and/or other small molecules or biomarkers. In some implementations, a small footprint low cost device provides rapid and robust sensing and identification. Such a device may utilize microfluidics, biochemistry, and electronics to detect one or more targets at once in the field and closer to or at the point of care. In some implementations, the systems and methods herein implement a reader device, an assay cartridge, and a mobile or external device configured to receive abiological sample, test the biological sample, and provide test results to a patient or user associated with the patient. The test results may be packaged with additional information, including symptoms suffered by the patient, a diagnosis, and follow-up instructions. In some embodiments, the test results may also be provided with or aggregated with other test results to generate aggregate information.

A test apparatus for a test using a test solution containing a sample with the use of a microchannel device is provided. The test apparatus includes a pressure application unit that is connected to a first opening and applies an air pressure to inject the test solution into a microchannel, an opening and closing unit that switches between opening and closing of a second opening, and a controller that controls the pressure application unit and the opening and closing unit. The controller controls the opening and closing unit to close the second opening, controls the pressure application unit to inject the test solution into the microchannel, controls the opening and closing unit to open the second opening after the test solution is injected into the microchannel, and controls the pressure application unit to apply an air pressure to collect in a collection portion, the test solution in a branch channel.

This invention provides devices, systems, and methods for performing point-of-care, analysis, including multiplexed analysis, of a biological fluid analyte, such as blood. The invention includes a cartridge for collecting the biological fluid analyte. The cartridge is configured to be inserted into an assay reader, in which one or more assay reactions may be performed. The assay reader is designed to read and report the results of the one or more assay reactions.

This disclosure relates to an apparatus for simultaneously filling a plurality of sample chambers. In one aspect, the apparatus comprises a common fluid source and a plurality of independent, continuous fluidic pathways. Each independent, continuous fluidic pathway comprises a sample chamber and a pneumatic compartment. The sample chamber is connected to the common fluid source, and the pneumatic compartment is connected to the sample chamber. The sample chamber comprises, in part, an assay chamber. The assay chamber comprises a monolithic substrate and a plug having optically transmissive properties. In some embodiments, the assay chamber contains a magnetic mixing element. In some embodiments, the assay chamber is a double tapered chamber. In some embodiments, a ratio of a volume of the sample chamber to a volume of the pneumatic compartment is substantially equivalent for each fluidic pathway of the plurality of fluidic pathways.

Embodiments of the invention relate to centrifugal fluidic devices, apparatus, and methods. Embodiments disclosed are fluidic devices, and associated apparatus and methods, which can partition a fluid sample from a single inlet or plurality of inlets into a plurality of chambers via their fluid inlets. Each chamber possesses a fluid outlet and a gas outlet. Partitioned fluid can be further distributed under centrifugal pressure to downstream fluidics modules, permitting various multiplexed assays to be performed including nucleic acid amplification tests.