A microfluidic chip orients and isolates components in a sample fluid mixture by two step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.

Provided are a microfluidic apparatus, a method and system for introducing a substance into a cell. The microfluidic apparatus includes a cavity channel, a bulk wave generating device and a surface acoustic wave generating device; a microstructure is arranged on an inner wall of the cavity channel, and the microstructure is constructed for forming a bubble by a solution at the microstructure when the solution is injected into the cavity channel; the bulk wave generating device is configured to generate a bulk wave, the bulk wave enables the bubble to resonate for generating a flow field; and the surface acoustic wave generating device is configured to generate a surface acoustic wave and control a position of at least one particle in the solution.

Provided are methods for biological sample processing and analysis. A method can comprise providing a substrate configured to rotate. The substrate can comprise an array having immobilized thereto a biological analyte. A solution comprising a plurality of probes may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector can be configured to detect a signal from the at least one probe coupled to the biological analyte, thereby analyzing the biological analyte.

A liquid handling device includes a common channel, a plurality of wells, a magnetic beads chamber and a plurality of valves. The plurality of valves are rotary membrane valves disposed on the circumference of a first circle. The magnetic beads chamber is disposed on a circumference of the second circle concentric with the first circle.

Provided is an apparatus for inducing and/or controlling flow of a fluid within a microchannel in a microfluidic device. The apparatus includes a fluid reservoir configured for holding a volume of fluid to be transported through said microfluidic channel and also configured for fluid connection to an inlet of said microfluidic channel. The apparatus also includes an evaporation reservoir configured for fluid connection to an outlet of said microfluidic channel. The evaporation reservoir includes at least one wetting, wicking or hydrophilic structure positioned at least partly within the reservoir. The wetting, wicking, or hydrophilic structure is capable of absorbing or conducting a fluid present in the microfluidic channel via wicking action or capillary force and maintaining a substantially constant volume of fluid in the evaporation reservoir. In use, evaporation of fluid at the outlet results in fluid being drawn from the fluid reservoir through the microfluidic channel to thereby create a flow of the fluid in the microfluidic channel.

The present invention relates to a micro-object extraction method using diffusiophoresis enabling collection and extraction of micro-objects by using the concentration difference of a solution including the micro-objects to be extracted, and a micro-object identification method using same, wherein the present invention has the following advantages: desired micro-objects can be easily extracted only with a simple device by using diffusiophoresis; the collection and extraction of micro-objects can be easily controlled by changing the type of solution injected into a micro-channel; and energy usage is efficient by using self-powered energy by diffusiophoresis without separate external power required for extracting micro-objects.

Systems and methods for detecting the presence of a target nucleic acid in a sample via a recombinase polymerase amplification (RPA) reaction followed by a FEN1 cleavage detection reaction are disclosed. One aspect of the present disclosure relates to systems involving a sample collection device for collecting a sample and performing an RPA reaction on the sample, followed by the detection of the amplified product via a two-step FEN1 cleavage detection reaction which generates a fluorescent signal indicative of the presence of amplified product.

The present disclosure relates to a mechanism for fluidic sealing of a reaction cartridge in a reaction system using a single linear actuator. A single motion provided by the linear actuator is used to establish leak-resistant fluid communication between the reaction cartridge and two independent fluidic channels. The dual-sealing assembly described herein enables the use of fewer parts and a simpler control unit. The use of fewer parts and simpler control system allow for a very compact sealing mechanism and could also increase reliability, will be easier to manufacture as it will require less manufacturing testing and calibration, and is more tolerant of variance in the part being sealed (the reaction cartridge). In some embodiments, the reaction cartridge comprises a solid support matrix and a reaction reagent attached to the solid support matrix, and the reaction system is used for treating macromolecules, such as polypeptides, for sequencing and/or analysis.

A system for selective microcapsule extraction includes a non-planar core-shell microfluidic device. The non-planar core-shell microfluidic device generates microcapsules defining a core-shell configuration. A subset of the microcapsules contain aggregates, tissues, or at least one cell. A camera captures images of the microcapsules. A detection module includes a processor and a memory. The memory includes instructions that when executed by the processor causes the detection module to provide the images of the microcapsules as an input to a machine learning model. The machine learning model identifies microcapsules containing aggregates, tissues, or at least one cell. A force generator generates a force to extract the microcapsules. A microcontroller selectively activates the force generator to generate the force when the detection module identifies a microcapsule containing aggregates, tissues, or at least one cell to extract the microcapsule.

A lateral flow assay device comprises a test strip to receive a quantity of fluid comprising a quantity of exosomes and detect the presence of a target analyte on the surface of the exosomes. The test strip comprises a conjugate pad that contains a set of one or more types of tetraspanin binding reagents conjugated with a label. Each type of tetraspanin binding reagent is configured to bind with a corresponding type of exosome tetraspanin and form an immunocomplex comprising an exosome. The conjugate pad is fluidly connected to a membrane. The membrane comprises a test line comprising an immobilized binding reagent to the target analyte. The immobilized binding reagent to the target analyte is configured to bind to a protein of the target analyte on the surface of an exosome in an immunocomplex comprising the exosome.