The invention discloses a microfluidic device for the culture, selection and/or analysis of sample organisms such as nematodes, as well as for other biological entities such as for instance animal embryos. The device features reservoirs, culture chambers and smart filtering systems allowing for the selection of specific populations/specimens of sample organisms, thus permitting long-term cultures thereof as well as phenotypic/behavioural analyses. Systems and methods for using the microfluidic device are within the present disclosure as well.

The present disclosure features portable wide field fluorimeter systems, e.g., in the form of low-cost mobile platforms, and methods to perform fluorometric assays to detect a change in fluorescence intensity in liquid samples, e.g., caused by the presence of a target analyte, e.g., a protein, e.g., an enzyme (e.g., β-lactamase) expressed by a target pathogen in a liquid sample in a point-of-care setting. In some implementations, a portable system for detecting a change in fluorescence intensity in a liquid sample includes a microfluidic device, an optical assembly including an emission filter and one or more lenses, and an analyzer device that collects and processes a fluorescent signal for the detection of a target analyte produced by the target pathogen present in the liquid sample.

Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereby forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents.

The present invention provides a method for the synthesis of an injectable composition comprising a [.sup.18F]-labelled pyridaben derivative that is advantageous over prior methods. In particular, the method of the present invention comprises a method of radiosynthesis that permits a more facile purification using solid phase extraction (SPE).

The invention relates to a device for analyzing solid biological elements and to a device for implementing same. The device comprises a plate (1) of tubes, the lower ends (2) of which are perforated and the upper ends (4) of which are open on the tube plate (1) to allow the introduction of an element to be analyzed (5), a deep-well plate (6) into which the tube plate (1) is inserted and a lifter (7) for raising the tube plate (1) from the deep-well plate (6). Each tube (3) in the tube plate (1) comprises at least one opening (9) toward its upper end (4) to allow air to pass through and each tube (3) can be closed at its upper end (4) with a stopper (8). The invention is applicable particularly in the medical, agri-food and forensic science fields.

A two-piece assembly for sequential through-matrix processing of solutions and/or solids is provided, the assembly having an inner vial which maintains and holds the matrix and an outer vial which is configured to receive the inner vial at the upper or lower parked positions, to respectively allow or impede passage of the solution through the matrix of the upper vial. Captured molecules can be treated with enzymes and/or chemistries in situ in the matrix, and without the need for the use of strong chaotropic agents such as urea or detergents like SDS.

Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and/or elution of fractions containing analytes.

Examples herein involve sorting a droplet including a biologic sample. In a particular example, sorting a droplet including a biologic sample includes generating a droplet including a biologic sample and a pH sensitive surfactant, and heating a nucleic acid molecule in the biologic sample. The pH sensitive surfactant may change the surface tension of the droplet responsive to amplification of the nucleic acid molecule. The droplet may be sorted into one of a plurality of sorting lanes based on the surface tension of the droplet, where a sorting lane among the plurality of sorting lanes is associated with droplets including the amplified nucleic acid molecule. A determination of whether the droplet includes the amplified nucleic acid molecule may be performed by detecting passage of the droplet in one of the plurality of sorting lanes.

A cartridge includes: a first reservoir capable of accommodating a sample liquid; a sheath liquid conduit; a sterilization filter; a mixer; a nozzle; a droplet collection member; and a check valve. The sterilization filter is provided at the sheath liquid conduit. The check valve is connected to a waste-droplet collection member. A sample liquid flow path and a sheath liquid flow path are isolated from a surrounding environment around the cartridge and are maintained in a sterile state. The sample liquid flow path extends from the first reservoir to the droplet collection member. The sheath liquid flow path extends from the sterilization filter to the droplet collection member.

The present invention relates methods for separation and/or concentration of cell nuclei and/or live cells from cellular and nuclear debris, and dead cells using magnetic levitation.