Provided is a method for manufacturing a medical diagnostic chip using a mixed lithography method. The method includes forming first patterns in a first region using first lithography, and forming second patterns in a second region using second lithography, wherein a part of the second patterns is formed in a part of the first region among the region where the first region and the second region are adjacent to each other.

Disclosed are devices and methods useful for confined-channel digital microfluidics that combine high-throughput droplet generators with digital microfluidic for droplet manipulation. The present disclosure also provides an off-chip sensing system for droplet tracking.

A microfluidic device including a serum separator, a quantum dot and antibody inlet connected to the serum separator, a quantum dot linked immunosorbent assay (QLISA) chamber connected to the serum separator, and an outlet connected to the QLISA chamber. The microfluidic device is configured to determine an amount of drug in a serum.

The present disclosure provides a cartridge for use in nucleic acid extraction and a nucleic acid extraction method which can simplify a nucleic acid extraction process and can be applicable to a point-of-care testing (POCT). The cartridge includes a chamber module, an air valve module, and a liquid valve module. The chamber module includes a plurality of chambers for extracting nucleic acids from a sample including a pretreatment chamber in which the sample is crushed and homogenization, cell disruption, and purification are performed. The air valve module is installed on the chamber module to control a pressure required to move a fluid between the plurality of chambers. The liquid valve module is installed below the chamber module to move the fluid between the plurality of chambers.

An embodiment for a microfluidic device is provided. The device comprises two areas, arranged side-by-side, and a trigger channel. They include a first area, which is delimited by a first liquid pinning barrier, and a second area, which is delimited by a second liquid pinning barrier. The latter extends parallel to the first liquid pinning barrier to delimit a corridor. The trigger channel extends through the corridor between the two areas. In addition, the trigger channel connects the first liquid pinning barrier with the second liquid pinning barrier, allowing a first liquid pinned at the first liquid pinning barrier and a second liquid pinned at the second liquid pinning barrier to be contacted, each, by a reverse flow of the second liquid in the trigger channel and thereby start mixing at a level of the corridor, in operation. The invention is further directed to related methods of operation.

The present invention relates to methods of conducting cholinesterase inhibition assays. In one instance, the assays can be configured to determine the presence of inactivated and activated cholinesterases. Also described herein are microfluidic devices and systems for conducting such assays.

Methods and devices for isolating microbial cells from a sample, extracting eukaryotic DNA from a sample, and identifying the microbial species in the sample are disclosed herein.

There is provided a microfluidic device (10) comprising a microfluidic structure having multiple spatially defined cell capturing channels (2) configured for enabling growth of cells or genetic libraries of cells or cell strains that are capable of producing or secreting compounds. The microfluidic structure of the microfluidic device (10) further comprises multiple spatially defined detection chambers (1; 1A) configured to receive and accommodate target entities. Each of the detection chambers (1) is configured for fluid connection with at least one of the cell capturing channels (2), and has a selective barrier (3A) defined between the detection chamber (1; 1A) and the respective cell capturing channel or channels (2) and adapted for allowing flow of at least one of the compounds from the respective cell capturing channel or channels (2) into the detection chamber (1; 1A) enabling the target entities in the detection chamber to be exposed to said at least one compound, while stopping cells or target entities from passing through the selective barrier (3A).

The present disclosure provides devices and methods using Plasma coil r separating a fluid—e.g., plasma or serum—from whole blood. In some embodiments, the devices and methods use hydrophobic or superhydrophobic surfaces to encourage whole blood to contact a selective membrane that extracts the desired fluid component from the blood.

The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.