A fluid separation apparatus is disclosed. The fluid separation apparatus includes a diluter which includes a first filter channel through which a fluid flows, a fluid structure formed to protrude outward from the first filter channel and including an air bag, and a first vibration generator configured to generate a first sound wave and which is configured to filter at least one substance included in the fluid based on a vortex of the fluid formed on an interface between the first filter channel and the air bag, and a separator which includes a second filter channel through which the diluted fluid flows, a second vibration generator configured to generate a second sound wave passing through the second filter channel, and a plurality of outlet channels branched from the second filter channel, wherein a plurality of substances included in the diluted fluid are separated based on the molecular weight.

A drug screening platform simulating hyperthermic intraperitoneal chemotherapy including a dielectrophoresis system, a microfluidic chip and a heating system is disclosed. The dielectrophoresis system is used to provide a dielectrophoresis force. The microfluidic chip includes a cell culture array and observation module and a drug mixing module. The cell culture array and observation module are used to arrange the cells into a three-dimensional structure through the dielectrophoresis force to construct a three-dimensional tumor microenvironment. The drug mixing module is coupled to the cell culture array and observation module and used to automatically split and mix the inputted drugs and output the drug combinations into the cell culture array and observation module. The heating system is used for real-time temperature sensing and heating control of the drug combinations on the microfluidic chip to simulate high-temperature drug environment when performing hyperthermic intraperitoneal chemotherapy on the three-dimensional tumor microenvironment.

A microfluidic device comprising a channel within a substrate and a condenser or a hydrodynamic focusing chamber along the channel, configured to focus a fluid containing particles of a plurality of sizes. A negative angle deterministic lateral displacement (DLD) array is configured to receive the focused fluid and separate the particles in the focused fluid into three sizes ranges. The negative angle DLD array comprises a plurality of rows of pillars, wherein the rows of pillars are positioned to repeat a pattern every N rows with a shift of M columns, N and M are relatively coprime, and N is greater than 1.

The present invention relates to vitro method for analysing a liquid sample as to the presence, identity and properties of microbes comprising: a) isolating microbes from the liquid sample; b) analysing said microbes spectroscopically by means of spontaneous Raman spectroscopy; and c) determining antibiotic susceptibility of said microbes spectroscopically by means of spontaneous Raman spectroscopy. The present invention also refers to device for analysing a liquid sample as to the presence, identity and properties of microbes, wherein the device comprises as a first unit (i) a chip comprising a filtering unit and an antibiotics exposure unit capable of determining the susceptibility of microbes to an antibiotic; as a second unit (ii) a Raman spectroscopy system; and as a third unit (iii) an evaluation module which is coupled to the Raman spectroscopy system.

Described herein is a microfluidic cartridge for purifying target particles or target cells of a predetermined size from contaminants in a sample, the cartridge comprising a first and a second planar support the first and second planar support each having a top surface and a bottom surface, wherein the top surface of the first and/or second planar support comprises at least one embedded channel extending from one or more inlets to one or more outlets; the at least one embedded channel comprising a plurality of obstacles, wherein the microfluidic cartridge comprises at least one void space configured to be deformed when assembling the first and second planar supports into the microfluidic cartridge.

A detection chip is disclosed. The detection chip includes a sample injection structure, a filter structure, and a reaction structure which are sequentially connected. The filter structure includes a first main body, and a first inlet portion and a first outlet portion respectively on two sides of the first main body. A width of the first inlet portion gradually decreases in a direction away from the first main body, and a width of the first outlet portion gradually decreases in a direction away from the first main body.

Provided is an integrated microfluidic device for SARS-CoV-2 detection. Also provided is a method for detecting SARS-CoV-2 by using the same, comprising viral lysis, RNA extraction, and reverse-transcription loop-mediated isothermal amplification (RT-LAMP). The integrated microfluidic device of the present disclosure is small in size, automatically operatable, and easy to use by ordinary people, and the present disclosure can achieve rapid detection with high sensitivity and specificity.

A fluidic coupler to engage a plurality of flow cells of a sensor device includes a body and a plurality of fluidics interfaces formed in the body. Each fluidic interface of the plurality of fluidics interfaces includes an opening, a first port in fluid communication with the opening, a second port, and a third port in fluidic communication with the second port.

A mesoscale fluidic system comprises a substrate having a sample chamber and an analysis chamber. The sample chamber comprises a cell permeable filter defining a sample application compartment and a conditioning medium compartment. The sample chamber has a sample inlet port in the sample application compartment. The analysis chamber has an entry port and an exit port. The conditioning medium compartment is in fluid communication with the entry port of the analysis chamber via a channel. The sample application compartment is below the cell permeable filter and the conditioning medium compartment is above the cell permeable filter. The mesoscale fluidic system is suited for analysing cellular motility in a sample. Also disclosed is a method of estimating the quantity of motile cells in a sample and a method of extracting motile cells from non-motile cells.

A point of use analyzer includes pump, valve, port, and storage channel. The storage channel may hold multiple assay packets composed of reagent aliquots separated by bounding slugs. The storage channel may define an elongated lumen having two ends with each of the ends coupled to the valve. A sampling device for use with the analyzer engages the port and may include a recurrent coaxial tube having a separation medium. A method of using the analyzer with the sampling device includes steps of pumping a fluid to displace a sample into the separation medium and out through the opposed connection.