This present disclosure provides devices, systems, and methods for performing point-of-care analysis of a target analyte in a biological fluid via a binding assay. The present disclosure includes a cartridge for collecting the target analyte contained in a fluid sample and performing an assay. The cartridge includes an assay stack having a first separation layer, a second separation layer, and a detection membrane. The cartridge also includes a plurality of first complexes comprising a capture molecule and a magnetic bead and a plurality of second complexes comprising a detection molecule and a detection label. Further, the detection membrane includes a substrate that interacts with the detection label to elicit a quantifiable response in the presence of the target analyte. The quantifiable response corresponds to an amount of detection antibody present in the detection membrane, and the amount of detection antibody present corresponds to an amount of the target analyte present.

The present embodiments relate to a fluid control device using centrifugal force. The fluid control device using centrifugal force includes a fluid control portion comprising a plurality of chambers and controlling a movement of a fluid inside the chamber; a lower fixing portion positioned on a lower portion of the fluid control portion and fixing the plurality of chambers; an upper fixing portion positioned an on upper portion of the fluid control portion and fixing the plurality of chambers; and a fastening member penetrating and fastening the lower fixing portion, the fluid control portion, and the upper fixing portion, wherein the plurality of chambers are disposed to face each other and placed on the lower fixing portion.

Devices, methods and systems are provided for isolating extracellular matrix bodies. More particularly, this invention discloses devices, methods and systems for isolating extracellular matrix bodies from a biological sample for use in diagnosis and prognosis of a subject. A device may have restriction channels for isolating extracellular matrix bodies and uniform flow channels for precise pressure measurements.

Spherical grains and sacrificial particles are mixed in a suspension. The sacrificial particles are larger than the spherical grains. The suspension is injected into a channel in a microfluidic chip, and the spherical grains form microporous structures in the channel. The microporous structures are sintered in the channel. A solvent is injected into the channel, and the solvent dissolves the sacrificial particles and forms macropores between at least some of the microporous structures, thereby forming a mixed-porosity microfluidic chip.

The present invention relates to a blood components collection and separation media (1) comprising a substrate (3) having a maximal flow pore size enabling the retention of at least red cells on the surface of the substrate (3), the blood components collection and separation media (1) comprises boundary walls (7) forming a pattern (9) and being made of a hydrophobic resin, and the pattern (9) presenting: a collection zone (91); at least one storage zone (93) aimed at collecting at least one component of the whole blood sample (5); and at least one channel (95) connecting the collection zone (91) to the at least one storage zone (93), the channel (95) forming a bottleneck between the collection zone (91) and the storage zone (93). The present invention further relates to a blood components collection and separation device and a blood components separation and extraction process.

A single junction sorter for a microfluidic particle sorter, the single-junction sorter comprising: an input channel, configured to receive a fluid containing particles; an output sort channel and an output waste channel, each connected to the input channel for receiving the fluid therefrom; a bubble generator, operable to selectively displace the fluid around a particle to be sorted and thereby to create a transient flow of the fluid in the input channel; and a vortex element, configured to cause a vortex in the transient flow in order to direct the particle to be sorted into the output sort channel.

The present invention discloses an electrophoretic chip comprising: (a) a non-conductive substrate designed to support elements of said electrophoretic chip; (b) an electrode structure for conducting current through said electrophoretic chip, printed on said non-conductive substrate and comprising a counter electrode and at least one working electrode, each electrode comprising a conductive low-resistance ink layer printed on the non-conductive substrate, and a carbon ink layer printed on top of and fully or partially covering said conductive low-resistance ink layer; (c) a dielectric ink insulator layer placed on top of, and covering, said electrode structure, said dielectric ink insulator layer having at least one opening above the counter electrode and at least one opening above said at least one working electrode, thereby forming at least one addressable location; and (d) a molecule capturing matrix spotted on and covering said at least one addressable location, thereby creating at least one microgel region.

Aspects of this disclosure relate to systems, devices, and methods for the transfer of fluid to fluidic receptacles. In some embodiments, the fluid contains one or more molecules (e.g., one or more peptides, proteins, and/or nucleic acids) of interest, and the fluidic receptacle includes an integrated device. In certain embodiments, the one or more molecules can be immobilized on the integrated device for subsequent analysis (e.g., sequencing). Certain aspects of the present disclosure are directed towards systems and methods that can, in some instances, enhance the immobilization of the one or more molecules on the integrated device, e.g., by improving a rate of sample interaction with the integrated device. Through the use of systems, devices, and methods of the instant disclosure, target molecules may be more readily sequenced or prepared for sequencing. For example, in some embodiments, systems, devices, and methods of the instant disclosure allow automated loading of the fluidic receptacle using a fluidic device.

The present disclosure provides a chip, a microfluidic device including the chip, and a method for sorting target droplets. The chip includes a first container for accommodating a first fluid, a second container for accommodating a second fluid, a delivery channel including a first flow channel communicating with the first container and a second flow channel communicating with the second container, the first flow channel and the second flow channel intersecting and communicating with each other at a junction, and at least one collector. A portion of the first flow channel includes the junction and is divided into two sections by the junction, in each section, the section thickens gradually along a first direction away from the junction. The second flow channel includes the junction and is divided into two sections by the junction, in each section, the section thickens gradually along a second direction away from the junction.

Devices, systems and methods are disclosed which relate to a prefiltration device that can be used with the concentrating pipette instruments and other devices which draw a sample in through one opening and dispense a concentrated or eluted sample out through the same opening. The device allows the sample to be passed through a prefilter when entering the opening and then bypassed the prefilter when being dispensed through the same opening.