A bag assembly for use with a heat exchanger includes a flexible bag having of one or more sheets of polymeric material, the bag having a first end that bounds a first compartment and an opposing second end that bounds a second compartment, a support structure being disposed between the first compartment and the second compartment so that the first compartment is separated and isolated from the second compartment. A first inlet port, a first outlet port, and a first drain port are coupled with the flexible bag so as to communicate with the first compartment. A second inlet port, a second outlet port, and a second drain port are coupled with the flexible bag so as to communicate with the second compartment.

A bag assembly for use with a heat exchanger includes a flexible bag having of one or more sheets of polymeric material, the bag having a first end that bounds a first compartment and an opposing second end that bounds a second compartment, a support structure being disposed between the first compartment and the second compartment so that the first compartment is separated and isolated from the second compartment. A first inlet port, a first outlet port, and a first drain port are coupled with the flexible bag so as to communicate with the first compartment. A second inlet port, a second outlet port, and a second drain port are coupled with the flexible bag so as to communicate with the second compartment.

A method for assembling a mixer-container intended for receiving a biopharmaceutical fluid includes providing a container with a mixing device including at least one shaft having an adjustable length, and at least one first bearing attached to a wall of the container, the shaft extending at least into the inner space from the first bearing; a rigid outer device that compresses the container; and a drive motor located outside the container, the first bearing of the container is placed so as to be spaced apart from the motor, and the length of the shaft is adjusted along the main axis by arranging the shaft opposite the motor to enable the motor to rotate the shaft.

In one aspect, a hydroformylation reaction process comprises contacting an olefin, hydrogen, and CO in the presence of a homogeneous catalyst in a cylindrical reactor to provide a reaction fluid, wherein the reactor has a fixed height, and wherein a total mixing energy of at least 0.5 kW/m3 is delivered to the fluid in the reactor; removing a portion of the reaction fluid from the reactor; and returning at least a portion of the removed reaction fluid to the reactor, wherein the returning reaction fluid is introduced in at least two return locations positioned at a height that is less than 80% of the fixed height, wherein the at least two return locations are positioned above a location in the reactor where hydrogen and carbon monoxide are introduced to the reactor, and wherein at least 15% of the mixing energy is provided by the returning reaction fluid.

A sparge for use in a high-pressure vessel operated at elevated temperatures and having high energy agitators for suspending mineral containing particles in a slurry. The sparge injects reagent fluids into the slurry to reduce reaction times and for controlling process parameters for extracting valuable minerals from the particles. The sparge has a vapour lock to inhibit the flow of particulate material and detritus material under low or no fluid flow situations which occur commonly in the operation of high pressure autoclaves. The sparge has a fluid flow path that increases in cross-sectional area in the direction of flow of reagent fluids so as to keep reagent fluids flowing at a velocity below a critical impingement velocity that can cause metal materials of the sparge to either wear rapidly, combust and in the worst case lead to loss of containment and violent and rapid depressurisation of the highpressure vessel.

A fluid transfer assembly for single use bioreactors includes a fluid transfer housing that can be mounted to the impeller shaft using a bearing that places the fluid transfer assembly directly below the lowest impeller but allows the impeller shaft to spin inside independently of the fluid transfer assembly. A fluid conduit connects the fluid transfer housing to a port in the single use bag wall which allows fluids to be introduced into the sparger and which also helps prevent the fluid transfer assembly from rotating with the impeller shaft.

A sparger assembly for a bioprocessing system includes a base and a plurality of spargers connected to the base, each sparger including a plurality of pores, the plurality of spargers each have a generally cylindrical shape. Each of the plurality of spargers includes a sidewall and a top, which define the cylindrical shape, the sidewall and the top each include a plurality of pores. The pores of the sidewall can be arranged around a circumference of the sidewall at an array of heights. Ridges may also be located on the sidewall above a respective array of pores.

A method for sterilizing by X-rays an assembly containing a single-use device intended to receive a biopharmaceutical fluid, comprising: placing a plurality of dosimeters, repeatedly passing the assembly in front of the X-ray radiation window, according to a first face then according to a second face of the assembly, at several irradiation power-time pairs (PTi), followed by mapping the radiation dose; and determining an optimum power-time pair (PTopt) for which the mapping reveals that all dosimeters have recorded a radiation dose above a minimum sterility dose (Dmin), and for which the dosimeter associated with a fragile element of the single-use device records a radiation dose below a maximum dose (Dmax) defined as being the dose from which the X-ray irradiation deteriorates the fragile element.

An impeller (18), for example, a Rushton impeller for a bioreactor system (10) is disclosed. The impeller (18) includes a hub (44), optionaly including a slot (168), a plurality of blades (46), and one or more turbulators (64). The plurality of blades (46) is disposed along a circumferential direction (48) of the hub (44) and spaced apart from each other. Each of the plurality of blades (46) is coupled to at least a portion of a circumference (50) and/or a top surface (1351;1451;1551) of the hub (44). Each blade of the plurality of blades (46) includes a pressure face (52) and a suction face (54). The one or more turbulators (64) is disposed on at least a portion of the suction face (54), the pressure face (52), or both, of a blade of the plurality of blades (46).

A method for assembling a mixer-container intended for receiving a biopharmaceutical fluid includes providing a container with a mixing device including at least one shaft having an adjustable length, and at least one first bearing attached to a wall of the container, the shaft extending at least into the inner space from the first bearing; a rigid outer device that compresses the container; and a drive motor located outside the container, the first bearing of the container is placed so as to be spaced apart from the motor, and the length of the shaft is adjusted along the main axis by arranging the shaft opposite the motor to enable the motor to rotate the shaft.