The invention provides for delivery, engineering and optimization of systems, methods and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.

The invention provides methods for rearing and genetic manipulation of the genome of sap-feeding insects (e.g., whiteflies and others) to identify genetic targets for pest control, insecticides for pest control, and approaches to the genetic control of these pests.

A method of enhancing larvicide susceptibility in a mosquito larva is provided. The method comprising introducing into the mosquito larva an isolated nucleic acid agent comprising a nucleic acid sequence which specifically reduces the expression of at least one larvicide resistance gene product of the larva, thereby enhancing larvicide susceptibility in said mosquito larva.

The present invention, in some embodiments thereof, relates to methods for enhancing fitness of pathogen-infected plants, and, more particularly, but not exclusively, to methods of using RNA interference for modulation of plant-pathogen resistance response gene expression. In particular, the present invention provides compositions and methods for enhancing host plant fitness and fruit yield and quality following Candidatus Liberibacter spp infection and, specifically, Candidatus Liberibacter spp infection in citrus plants and trees, as in Huang Long Bing.

Disclosed herein are methods and compositions for genome editing of a Rosa locus, using fusion proteins comprising a DNA binding domain and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.

The purpose of the present invention is to provide a set of two polypeptides for use in light-dependent genetic recombination in which the N-terminal side fragment and the C-terminal side fragment of a Cre protein having an amino acid sequence of SEQ ID NO: 1 respectively bind to two proteins light-dependently forming a dimer.

Described herein are methods and compositions for generating single sex offspring using enhance trans-splicing approach via an RNA binding framework. In particular, methods and compositions are provided to generate single sex and genetically modified offspring. These techniques can be applied to compassionate animal breeding.

The invention provides methods for rearing and genetic manipulation of the genome of sap-feeding insects (e.g., whiteflies and others) to identify genetic targets for pest control, insecticides for pest control, and approaches to the genetic control of these pests.

Disclosed herein is a genetically modified mouse whose genome comprises a transgene encoding a Miltenberger blood group antigen subtype III (Mi.II antigen). According to embodiments of the present disclosure, the Mi.III antigen comprises the amino acid sequence of SEQ ID NO: 1. Also disclosed herein are a method of producing the genetically modified mouse, and uses of the genetically modified mouse in selecting a drug candidate for treating hypertension.

Described herein are anchor-modified immunoglobulin polypeptides, wherein the anchor moors the immunoglobulin polypeptide to a receptor of interest. The anchor-modified immunoglobulin polypeptides are generally characterized at the N-terminus with an anchor, e.g., the receptor binding portion of a ligand that binds a receptor. Non-human animals genetically modified with recombinant immunoglobulin segments that encode the anchor-modified immunoglobulin polypeptides are capable of making the anchor-modified immunoglobulin polypeptides. Such non-human animals also provided, along with methods and compositions for making and using the non-human animals. Methods for producing anchor-modified immunoglobulins from non-human animals are also provided, as well as anchor-modified immunoglobulins generated therefrom.