The present invention provides a method for specifically cleaving a Cα-C bond of a peptide backbone and/or a side chain of a protein and a peptide, and a method for determining amino acid sequences of protein and peptide. A method for specifically cleaving a Cα-C bond of a peptide backbone and/or a side chain bond of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of at least one hydroxynitrobenzoic acid selected from the group consisting of 3-hydroxy-2-nitrobenzoic acid, 4-hydroxy-3-nitrobenzoic acid, 5-hydroxy-2-nitrobenzoic acid, 3-hydroxy-5-nitrobenzoic acid, and 4-hydroxy-2-nitrobenzoic acid. A method for determining an amino acid sequence of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of the above specific hydroxynitrobenzoic acid to specifically cleave a Cα-C bond of a peptide backbone and/or a side chain bond, and analyzing generated fragment ions by mass spectrometry.

Methods of modifying renewable protein sources and uses thereof are provided. In some embodiments, renewable protein sources can be modified to become a flocculant and/or coagulant through the use of a hydrolysis process. Further modifications can be performed in order to enhance the flocculant/coagulant ability of the modified protein material. Such modified protein material can be used to coagulate and/or flocculate waste water colloidal suspensions, either alone or in combination with a coagulant, by mixing the modified protein material with waste water colloidal suspensions to create a mixture and allowing mixture to settle. In some embodiments, the waste water colloidal suspension can be mature fine tailings (MFT).

Methods of modifying renewable protein sources and uses thereof are provided. In some embodiments, renewable protein sources can be modified to become a flocculant and/or coagulant through the use of a hydrolysis process. Further modifications can be performed in order to enhance the flocculant/coagulant ability of the modified protein material. Such modified protein material can be used to coagulate and/or flocculate waste water colloidal suspensions, either alone or in combination with a coagulant, by mixing the modified protein material with waste water colloidal suspensions to create a mixture and allowing mixture to settle. In some embodiments, the waste water colloidal suspension can be mature fine tailings (MFT).

The present invention relates to a method for hydrolyzing eggshell membrane, comprising the step of treating a suitable amount of eggshell in a solution containing a denaturing agent, a reducing agent, a buffer, and an enzyme. The invention also relates to a composition for hydrolyzing eggshell membrane according to the preceding method.

The present invention relates to a method for hydrolyzing eggshell membrane, comprising the step of treating a suitable amount of eggshell in a solution containing a denaturing agent, a reducing agent, a buffer, and an enzyme. The invention also relates to a composition for hydrolyzing eggshell membrane according to the preceding method.

Described herein is an operationally simple, one-pot solid-supported preparation of saturated stapled peptides. Following completion of ruthenium-catalysed metathesis, solid-phase transfer hydrogenation was achieved using triethylhydrosilane at elevated temperatures. The utility of the method has been demonstrated on 14- and 16-mer peptides to yield the corresponding cyclic a-helix stabilised stapled peptides.

Described herein is an operationally simple, one-pot solid-supported preparation of saturated stapled peptides. Following completion of ruthenium-catalysed metathesis, solid-phase transfer hydrogenation was achieved using triethylhydrosilane at elevated temperatures. The utility of the method has been demonstrated on 14- and 16-mer peptides to yield the corresponding cyclic a-helix stabilised stapled peptides.

The present invention relates to the field of medicinal synthesis, and discloses a method for synthesizing degarelix. The method of the present invention as a whole divides the synthesis of degarelix into two parts from amino acids at positions 5 and 6, employs proper protective groups in part of the protected amino acids therein, and finally uses in association with a specific acidolysis agent to complete the whole synthesis process. In the present invention, a proper synthesizing scheme is selected, and adaptive protective group and acidolysis agent are selected, so that the overall synthesis process is optimized, the purity of degarelix is significantly improved with a higer total yield, and the production of the toxic hydantoin degradation product is avoided.

The present invention relates to a novel insulin analog, use thereof, and a method for preparing the analog.

The present invention relates to a novel insulin analog, use thereof, and a method for preparing the analog.