An apparatus for culturing cells of the present disclosure includes: a cabinet that has a main surface and a side surface, the main surface having a window; a main surface aspiration port disposed at the main surface of the cabinet; and a side surface aspiration port disposed at the side surface of the cabinet. In the cross section of the cabinet taken along the horizontal direction, a first vessel supplying part for supplying a first vessel is disposed at a midportion of the cabinet, and a second vessel supplying part for supplying a second vessel is disposed at an inner peripheral portion of the cabinet. The first vessel supplying part has a first lid capable of entirely covering the first vessel supplying part. The first vessel has no lid. The second vessel supplying part has no lid. The second vessel has a second lid.

A method for observing biological species on a culture medium contained in a container having at least one translucent face, the method including the steps of: a) directing a light beam onto one portion of the translucent face, so as to define at least one illuminated region and at least one non-illuminated region of the face; and b) acquiring an image of a portion of the surface of the culture medium illuminated by the light beam, the acquisition being carried out through at least one of the non-illuminated regions of the translucent face and along an optical acquisition axis forming a non-zero angle (a) with the direction of propagation of the light beam.

Means which enables preparation of a thick cell aggregate by a simple process without an operation of detaching and stacking of cells is disclosed. The method for preparing a three-dimensional cell aggregate by the present invention comprises: a cell encasing step of placing a cell suspension in a cell container; and a pressure application step of applying pressure to cells in the container. The cell encasing step and the pressure application step may be carried out a plurality of times. By the present invention, a thick, robust cell aggregate can be obtained by a simple operation of applying pressure to a cell suspension or a medium containing cells.

Since the method does not require an operation of stacking a plurality of cell sheets, the cells are hardly damaged, and the conditions of the cells can be favorably maintained, so that the cells can be advantageously used as a tissue piece for transplantation.

A petri dish has interlock features between the lid and dish that allow single-handed engagement of the lid with the dish by rotation, so that the lid and dish may be raised by grasping only the lid without stabilization of the dish with a second hand. Disengagement is similarly single-handed.

The invention relates to a medium-filled Petri dish into which filters can be introduced particularly simply, and to a method for the microbiological investigation of liquids or gases by means of filtration and incubation of the filters in said Petri dishes.

A semipermeable membrane includes a holding body with a low water absorption property having a lattice structure and having a semipermeable property in a liquid phase. A cell-culturing device is provided with the semipermeable membrane at least at a portion thereof. A tissue-type chip is provided with the cell-culturing device including one type of cells. An organ-type chip is provided with the cell-culturing device including at least two types of cells. A kit for providing a multicellular structure is provided with an openable and closable sealed container including the tissue-type chip or, and a culture medium. An organ-type chip system is provided with at least two of the tissue-type chips or the organ-type chips, and the tissue-type chips or the organ-type chips are connected while maintaining a sealing property. A cell-culturing method is a method of using the cell-culturing device.

Provided is a resin film having excellent cell extensibility in cell culture. The resin film formed of a scaffold material for cell culture containing a synthetic resin, the resin film having a compressive modulus of 5.5 GPa or more at a frequency of 1 Hz as measured using a nanoindenter device in ion-exchanged water in accordance with ISO14577-1 after being immersed in the ion-exchanged water at 37° C. for 24 hours.

A device for centering of petri dishes, where the petri dishes include a bottom container with a lateral surface and a lid with a lid diameter, including an elevator with an elevator axis and an elevator drive, and the conveyor includes concentrically along the elevator axis a frustoconical through-hole tapering downwards, and the elevator drive is built to move a substantially flat plate from a neutral position downwards into a first position, which is reached as soon as the petri dish rests with its weight on the inner surface of the through-hole, and the elevator drive is built to rotate the plate during a movement of the plate from the neutral position into the first position.

The invention relates to a pro-biofilm coating applied by means of cold atmospheric plasma polymerization of a precursor on a substrate. The coating has a roughness such that it promotes the creation of more than 100% biofilm on the substrate, where the 100% of biofilm is the one as produced on the same substrate being devoid of said pro-biofilm coating. The invention also relates to a method of producing said pro-biofilm coating and a substrate coated with same.

A segmented culture dish and imprinting system allows duplicate plates to be produced rapidly and without culture-to-culture overlap. The segmented culture dish and imprinting system includes a dish stamp, a first culture dish, and a second culture dish. The dish stamp may be placed on the first and/or the second culture dishes. The first and the second culture dishes have a key on an inside surface that ensures the orientation of cultures is always preserved, eliminating the need for markings on culture dishes.