The membrane bioreactor for simultaneous enzymatic cellulose hydrolysis and product separation is a vessel having a lower hydrolysis reaction chamber and an upper distilled water chamber separated by a semipermeable membrane attached to the top of the lower reaction chamber. The membrane is supported on a stainless steel mesh and sealed to the mesh by epoxy glue to prevent leakage. A peristaltic pump is connected to the reaction chamber and maintains a flow of distilled water through the membrane and the upper chamber, the effluent being collected in a beaker or other product collection vessel. The reaction chamber is agitated at a moderate rate by a magnetic stirrer, and the upper chamber is agitated more rigorously by a mechanical stirrer. A thermocouple and temperature controller and a buffer solution, respectively, maintain temperature and pH in the reaction chamber optimal for enzymatic hydrolysis of cellulose.

A sample collection device including a collection tube at least partially defining a storage volume therein, a mouthpiece coupled to the collection tube, the mouthpiece defining a channel providing access to the storage volume, and a filter at least partially positioned within the channel, where the filter is configured to filter a sample as it passes through the channel and into the storage volume.

The disclosure provides systems and methods for producing a cannabinoid product, which comprises contacting a cannabinoid precursor in a first phase with a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible. The disclosure also provides a composition comprising the cannabinoid precursor in a first phase and a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible.

The disclosure provides systems and methods for producing a cannabinoid product, which comprises contacting a cannabinoid precursor in a first phase with a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible. The disclosure also provides a composition comprising the cannabinoid precursor in a first phase and a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible.

Methods of decellularization of tissue, such as mammalian tissue, are provided, along with methods of making an extracellular matrix (ECM) preparation. Systems and apparatus useful in performing the methods are also provided.

A nucleic acid synthesis device and a nucleic acid purification device, uses thereof, and a nucleic acid synthesis method and a nucleic acid purification method. The nucleic acid synthesis device includes a solid support, and the solid support includes a controlled pore glass (CPG), the CPG is an unmodified and bare CPG, a surface of the CPG has a hydroxyl group, and the hydroxyl group is attachable, though a covalent bond, to a phosphoramidite-protected nucleotide monomer or multimer for synthesis of nucleic acid. The nucleic acid synthesis device of the present disclosure can be used for not only synthesis of an oligonucleotide primer, but also for purification of enzymatic digestion and PCR product by using the oligonucleotide primer immobilized on the CPG, and has advantages of simple structure, small volume, light weight, high efficiency, low costs, and diversified functions.

A membrane structure for moving a gaseous object species from a first region having an object species first concentration, through the membrane structure, to a second region having an object species second concentration different from the first concentration is described. The membrane includes a supporting substrate having a plurality of pores therethrough, each of the plurality of pores defined by a first end, a second end and a surface of the supporting substrate extending between the first end and the second end as well as a nanoporous layer within the plurality of pores, wherein the nanoporous layer comprises a hydrophilic layer and a hydrophobic layer. The membrane also includes a liquid transport medium within the hydrophilic layer. The liquid transport medium includes a liquideous permeation medium and at least one enzyme within the liquideous permeation medium. The at least one enzyme is reinforced by at least one stabilizing component.

A process and apparatus for the enzymatic degradation of a cellulosic substrate is disclosed. The process comprises agitating a composition with milling particles, wherein the milling particles are or comprise a lignocellulosic material and wherein the composition comprises: a. the cellulosic substrate; b. a cellulase enzyme; and c. a liquid medium.

The present disclosure provides a system for mimicking the secondary lymphoid organs where suspension cells (e.g., T cells) are expanded; methods el expanding activating, and transfecting the suspension cells in the synthetic, microenvironment, and suspension cells produced by such systems and methods.

The present invention provides a system for recovering protein in a production process of an ultrahigh maltose syrup, including a saccharification tank, an enzyme preparation tank, a first plate heat exchanger, a second plate heat exchanger, a plate and frame filter, a buffer tank and a rotary drum filter. The present invention further provides a method of recovering protein by using the system. After the sugar liquid in the saccharification tank is stood, the protein floats at the upper part of the saccharification tank and the lower liquid is clear and transparent and thus the sugar liquid can be directly filtered. When the saccharification tank is discharged, the lower liquid is firstly discharged with the remaining liquid being bottoms containing protein. During a production process, enzymatic hydrolysis is performed for the sugar liquid containing protein before filtration to improve the filtration effect of the sugar liquid. Assisted by the plate and frame filter, protein can be recovered. The present invention improves the economic benefits, reduces the production costs, and thus solves the problem of difficulty in recovering protein in a production process of an ultrahigh maltose syrup.