The present disclosure relates to algae cultivation including the integration of liners for large-scale open algal biomass to facilitate algae facility commercial scale-up operations. Liners include those having a first portion having a first thickness and a second portion comprising a second thickness, wherein the first thickness and the second thickness are different. Liners also include those having a first portion having a first thickness, a second portion comprising a second thickness, and a third portion comprising a third thickness, wherein the first thickness, the second thickness, and the third thickness are different.

The invention relates to a pro-biofilm coating applied by means of cold atmospheric plasma polymerization of a precursor on a substrate. The coating has a roughness such that it promotes the creation of more than 100% biofilm on the substrate, where the 100% of biofilm is the one as produced on the same substrate being devoid of said pro-biofilm coating. The invention also relates to a method of producing said pro-biofilm coating and a substrate coated with same.

A biological component treatment system according to embodiments of the present disclosure includes an IC route through which the liquid is capable of flowing in inner cavities of hollow fibers, and an EC route through which the liquid is capable of flowing in a main space on an outer side of the hollow fibers. The biological component processing system is further equipped with a control unit that controls a flowing state of the liquid through the IC route and the EC route. The control unit, at a time of priming, while the liquid flows through both the IC route and the EC route, causes a differential pressure to be generated between the liquid flowing through the inner cavities and the liquid flowing through the main space, and causes the gas to flow out from the hollow fibers.

The disclosure relates generally to a cell culture apparatus and a cell culture method. One aspect of the disclosure is an oxygen-permeable bag comprising one or more polymer films defining a boundary of an interior compartment of the bag, each of the one or more polymer films comprising an inner layer adjacent the interior compartment of the bag, the inner layer comprising a fluoropolymer, and adhered to the inner layer, an outer layer comprising polymer; a first port formed in an exterior surface of the bag and in fluid communication with the interior compartment; a second port formed in an exterior surface of the bag and in fluid communication with the interior compartment; and contained in the interior compartment, a plurality of microcarriers.

The present disclosure provides a substrate for cell culture. Systems comprising the substrate, and methods for using and manufacturing the substrate are also disclosed herein.

By using a graft polymer comprising a dendritic polymer with a styrene skeleton and a hydrophilic polymer grafted to a terminal thereof, a temperature-responsive substrate for cell culture having a temperature-responsive surface for cell culture that allows cells to be cultured with high efficiency and which yet allows cultured cells to be exfoliated in a short period of time and with high efficiency by simply changing the temperature of the substrate surface can be prepared conveniently. If this temperature-responsive substrate for cell culture is used, cells obtained from a variety of tissues can be cultured with high efficiency. If this culture method is utilized, cultured cells can be exfoliated intact in a short amount of time with high efficiency. In addition, by using this graft polymer, a wide range of peptides and proteins can also be separated by simply changing the temperature of a chromatographic carrier. This allows for convenient separation procedure and improves the efficiency of separating operations. What is more, the stereoregularity of the dendritic polymer per se may be utilized to enable separation of solutes based on differences in their molecular structures.

Photoreceptor scaffolds and scaffold systems including the photoreceptor scaffolds are described herein. The scaffolds and scaffold systems can be used for transplantation of organized photoreceptor tissue, with or without RPE, which may improve grafted cell survival, integration, and functional visual rescue. Particularly, the photoreceptor scaffold is structured from a biocompatible film, patterned with an array of unique through-holes having a curvilinear cell receiver and at least one cell guide channel.

A device for seeding cells includes a container with a wall, a bottom and a lid. The wall extends between the bottom and the lid. The container can be equipped to be loaded with cells, in particular with cells form a cell suspension. The container defines a rotation axis. The device is further equipped to rotate the container around the rotation axis. The container includes a structured surface that can be arranged at the inner surface of the container. The structured surface has structures equipped to receive the cells. The rotation exerts a (g-)force in direction of the structured surface, such that the g-force acts perpendicular to the structured surface. The exerted force in the direction of the structured surface resembles a g-force required for sedimentation of the cells into the structures.

A cell culture chip comprises a body having a first flow channel and a second flow channel at least part of which overlaps the first flow channel when seen from an angle parallel with a predetermined direction, a cell separation membrane having first and second principal surfaces facing away from each other, the cell separation membrane being disposed between the first flow channel and the second flow channel so that the first flow channel is located on the first principal surface and the second flow channel is located on the second principal surface, a first electrode which is in contact with the first flow channel and extending through the first flow channel along the first flow channel, and a second electrode which is in contact with the second flow channel and extending through the second flow channel along the second flow channel.

The present invention is drawn to the generation of micropatterns of biomolecules and cells on standard laboratory materials through selective ablation of a physisorbed biomolecule with oxygen plasma. In certain embodiments, oxygen plasma is able to ablate selectively physisorbed layers of biomolecules (e.g., type-I collagen, fibronectin, laminin, and Matrigel) along complex non-linear paths which are difficult or impossible to pattern using alternative methods. In addition, certain embodiments of the present invention relate to the micropatterning of multiple cell types on curved surfaces, multiwell plates, and flat bottom flasks. The invention also features kits for use with the subject methods.