The present invention relates to a large-scale photosynthetic bioreactor in which transparent photobioreactors including a baffle are connected in parallel or in series by an adhesive element so that the reactor volume can be easily scaled up for scale-up culture of photosynthetic organisms, and to a fabrication method thereof. The large-scale photosynthetic bioreactor according to the present invention makes it possible to culture a larger amount of microalgae than a conventional photobioreactor in the same area. In addition, it has high light transmittance, produces a large amount of biomass per unit area due to smooth mixing, and has a significant effect of reducing carbon dioxide. Furthermore, the present invention has an advantage in that the number of photosynthetic bioreactors required to culture the same scale of photosynthetic organisms is significantly smaller than that in a conventional process for culture of photosynthetic organisms, and thus the operating costs can be reduced.

Methods, systems, and apparatus for cultivating cells in media-exchanging wells. In an exemplary method of cell cultivation, a device (50) may be selected that includes a row of wells (52), and a first reservoir (56) and a second reservoir (58) located at opposite ends of the row of wells (52). Each well (52) may have a lower portion and an upper portion. The lower portion of each well (52) of at least two of the wells (52) may contain a cell culture in contact with a liquid. Liquid may be transferred between the first reservoir (56) and the second reservoir (58) at least partly along a flow path (62) defined by the device and extending through the upper portion of each well (52) of the row of wells (52), such that molecules of the media are exchanged between or among the at least two wells (52).

In one embodiment, an algae cultivation system includes a basin that contains a liquid and a photobioreactor at least partially immersed in the liquid of the basin, the photobioreactor comprising a closed container including multiple panels that together define an interior space in which algae can be cultivated, at least one of the panels being transparent, the photobioreactor further comprising an inflatable float associated with the container that can be filled with a gas to change one or both of the position and orientation of the container within the liquid.

The described invention provides an ex vivo dynamic multiple myeloma cancer niche contained in a pumpless perfusion culture device. The dynamic multiple myeloma cancer niche includes (a) a three-dimensional tissue construct containing a dynamic ex vivo bone marrow niche, which contains a mineralized bone-like tissue containing viable osteoblasts self-organized into cohesive multiple cell layers and an extracellular matrix secreted by the viable adherent osteoblasts; and a microenvironment dynamically perfused by nutrients and dissolved gas molecules; and (b) human myeloma cells seeded from a biospecimen composition comprising mononuclear cells and the multiple myeloma cells. The human myeloma cells are in contact with osteoblasts of the bone marrow niche, and the viability of the human myeloma cells is maintained by the multiple myeloma cancer niche.

The invention provides a structured cell growth matrix or assembly comprising a one or more spacer layers and one or more cell immobilization layers. The invention further provides a bioreactor comprising said matrix or assembly.

A culture device and a culture method by which a substance to be cultured can be three-dimensionally cultured, and the substance thus cultured can be removed as an integrated product with multiple tubes; and a cultured organ produced by the culture method. The culture device comprises a sealed container (2) which contains the substance to be cultured (A) and is disassembled after the culture is completed; multiple ducts (3, 4, 5) arranged in the sealed container (2) and have micropore formed on the outer peripheral surface; a culture medium-feeding device (6, 7) for feeding/circulating the culture medium (B) to at least one duct (3 or 5); an excretory device (8) connected to at least one duct (4) for excreting the waste product (C) permeating into the duct (4) through the micropore of the duct (4) from the substance to be cultured to the outside of the sealed container.

The invention relates to a bioreactor (10, 10′, 100) adapted for rotation, the bioreactor comprising: a vessel (12) comprising: a first end (24) and a second end (26) which define a central axis (28) of the vessel (12) extending along a first direction, e.g. a length direction, of the vessel (12) from said first (24) to said second end (26), at least one wall (18, 18′, 20) running along the first direction of the vessel (12), at least one media conduit (22, 22′) defining a volume for receiving fresh or spent media; an inner chamber defined by at least a part of a space confined within said at least one wall (18, 18′, 20) and comprising a fresh media chamber (14) and a spent media chamber (16); a cell culture chamber (30) in fluid communication with said at least one media conduit (22, 22′) and said fresh (14) and/or spent media chamber (16); and a movable wall (38) configured, within said inner chamber, to separate said fresh media chamber (14) from said spent media chamber (16) within said inner chamber.

A device for growing microorganisms. The device includes a body member comprising a self-supporting, water-proof substrate having upper and lower surfaces; a hydrophobic spacer element adhered to the upper surface of the substrate forming side walls to retain a predetermined amount of liquid in contact with the substrate, wherein the hydrophobic spacer element has a hole therein; a fluid control film in the hole of the hydrophobic spacer element; a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the body member; and a substantially dry, first microbial growth nutrient composition disposed on a portion of the inner surface of the cover sheet; a first adhesive composition adhered to the first microbial growth nutrient composition; and a cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition.

A glass composite is provided that has a first and second glass element, each having a first surface, and a first coupling agent layer having a first and second silane coupling agent. The first coupling agent layer has covalent bonds between the first and second silane coupling agents. The first and second silane coupling agents are covalently bonded to the first surface of the first and second glass elements, respectively. The first and second glass elements are irreversibly connected by the first coupling agent layer. Such a glass composite is made by bonding the first surface of the first and second glass elements to the first and second silane coupling agents, respectively, and contacting both first surfaces with each other to cause the first and second silane coupling agents thereon to covalently bond so that the first and second glass elements are irreversibly connected.

A bioreactor capable of producing complex, three-dimensional tissue constructs has improved media transfer and increased controllability with regard to exposure to the external environment. The bioreactor includes an external surface, a first tissue culture side for culturing a first cell source in a first tissue culture support region, a second tissue culture side for culturing a second cell source in a second tissue culture support region, and a plurality of ports. At least one of the ports of the plurality of ports extend from the first tissue culture support region of the first tissue culture side to the external surface. The plurality of ports can include an external port that is configured to be a liquid inlet when the bioreactor is in a first orientation and a gas outlet when the bioreactor is in a second orientation.