The present invention relates to a hybrid system of a culture and purification process for manufacturing antibody pharmaceuticals, in which one or more devices selected from bioreactor, chromatography, and filtration equipment are provided with one or more materials selected from among SU disposable bags and stainless steel (SS), and one or more devices selected from preparation and storage tanks of a medium and a buffer are provided with SS material, thereby simultaneously obtaining the advantages of a SS process system and a SU process system, and preventing cross-contamination through independent operation of a culture unit and a purification unit, and a process system is designed and positioned such that an effluent stream exiting individual culture units is introduced into one or more purification units using a control unit, thereby avoiding a bottleneck phenomenon from occurring in the purification process.

Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency.

A variable diameter bioreactor vessel is provided that includes a first vessel section having a first diameter configured to hold a liquid media and biologic material, and a second vessel section having a second diameter that is greater than the first diameter such that the liquid media can be increased from a first volume to a second volume within the vessel.

Disclosed herein is an instrument suitable for processing cells for example culturing, concentrating or washing said cells, the instrument comprising: a housing for accommodating mechanical elements including at least one fluid pump; and a disposable processing kit complementary to the mechanical elements within the housing and comprising a fluid circuit including a fluid reservoir and plural fluid paths capable of carrying fluid flow caused by said pump(s), the instrument further including a mechanism for determining the quantity, or change in quantity of the fluid in the reservoir resulting from said fluid flow, the instrument yet further comprising a controller operable to control at least the pump and operable to perform a fault determination process, which includes the steps of determining the expected flow rate of said pump(s) calculated from the speed of the pump(s) and comparing that expected flow with the change in quantity of the fluid in the reservoir as determined by said mechanism.

A multilayer bioreactor bag (20) made from a multilayer film (10), the bag having: inner and outer exterior surface layers (11, 16) on opposing faces of the multilayer film (10) consisting essentially of a low density polyethylene having a density in a range of 0.91 to 0.94 g/cc to provide a non-tacky exterior surface layers; interior layers disposed between the exterior surface layers comprising a gas barrier layer (14) and a structural layer (12) comprising an ethylene alpha-olefin elastomer having a density in a range of 0.87-0.91 to provide a flexible support to the multilayer film.

The invention discloses a first unit (1) for treatment of a bioprocess liquid comprising a first lateral face (2), a second lateral face (3) and a front face (4) which meets the two said lateral faces. The front face comprises: a plurality of valves (7) adapted to receive and act upon one or more legs (8) of a disposable flow path (6); optionally one or more pumps (10) adapted to receive and act upon one or more legs of the disposable flow path; optionally one or more sensors (11) adapted to receive and to measure one or more parameters in one or more legs of the disposable flow path; wherein the plurality of valves and optional pumps and sensors are vertically offset from each other to give one or more legs of a disposable flow path received by said valves and optional pumps and sensors a slope of at least 3.0 degrees from the horizontal plane (h).

This disclosure relates to reaction container systems providing for headspace-based condensation, coalescing devices, and other features.

The present application relates to a bioreactor system for culturing cells, especially cells without cell walls; the bioreactor system includes: a container containing a hollow cylinder with a diameter of D1 and a height of H1, and a hollow circular truncated cone with an upper diameter of D2, a lower diameter of D3, and a height of H2, where the hollow cylinder is connected to a top surface of the hollow circular truncated cone, and D1=D2; an oscillator, configured to cause the container to make an eccentric motion according to a certain eccentricity and rotational speed; a ventilation device, configured to introduce an oxygen-containing gas from an upper portion of the container to the inside of the container, and a culture solution filled in the container, of which a top surface is exposed to the oxygen-containing gas; where the oscillator is configured to maintain the eccentric motion of the container, such that a ratio of the total liquid surface area to the volume (S/V) of the culture solution when in a steady state of motion is 5.65 or more, the turbulence kinetic energy is 2.73E−03 m.sup.2/s.sup.2 or more, and the flow field shear rate is 20.27/s or less, where the total liquid surface area is the sum of the contact area between the culture solution and the reactor wall surface and the contact area between the top surface and the gas.

A device for detachably fastening a sensor for determining and/or monitoring at least one process variable of a medium to a single-use container, wherein there is a connection between the device and a wall of the single-use container, comprises a fastening unit for detachably fastening the sensor to the single-use container. Disclosed also is a single-use container having a device according to the invention.

An illuminated container for the growth of biological entities is provided. The container is illuminated by a flexible light diffusing fiber. The light diffusing fiber includes a core formed from a silica-based glass and a cladding in direct contact with the core. The light diffusing fiber also includes an outer polymer coating layer surrounding the cladding, the outer polymer coating layer being the cured product of a liquid polymer blend including a scattering material and a luminophore.