The present disclosure relates to a cell culture apparatus, methods for cell cultivation by using the cell culture apparatus, and a cell culture incubator comprising the cell culture apparatus. The cell culture apparatus comprises a plurality of cell culture modules arranged adjacent to each other. Each cell culture module comprises two or more culture medium reservoirs connected by two or more flow channels. The flow channels go through a basal chamber arranged under an apical chamber. The apical and basal chambers are separated by a porous membrane. The bottom part of the at least two culture medium reservoirs comprise a holding member configured to be compatible with a liquid handling system. The basal chamber has a bottom part arranged higher than a bottom part of each of the culture medium reservoirs. The cell culture apparatus further comprises a cavity under the bottom part of the basal chamber.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Compositions and methods for a hybrid biological and chemical process that captures and converts carbon dioxide and/or other forms of inorganic carbon and/or CI carbon sources including but not limited to carbon monoxide, methane, methanol, formate, or formic acid, and/or mixtures containing CI chemicals including but not limited to various syngas compositions, into organic chemicals including biofuels or other valuable biomass, chemical, industrial, or pharmaceutical products are provided. The present invention, in certain embodiments, fixes inorganic carbon or CI carbon sources into longer carbon chain organic chemicals by utilizing microorganisms capable of performing the oxyhydrogen reaction and the autotrophic fixation of CO.sub.2 in one or more steps of the process.

To provide a closed system culture vessel for realizing culturing in which an aseptic state is maintained at the time of culturing, and making the reduction of the shear stress generated during conveyance after manufacturing possible. A closed system culture vessel 201 holds a second vessel 302 consisting of an insert vessel on the inside of a first vessel 301, and a lid portion 303 of the closed system culture vessel 201 forms a closed space with the first vessel 301 and has a convex portion 305 contacting the entire outer circumference of the second vessel 302. During conveyance, the closed system culture vessel is conveyed in a state in which the culture medium for conveyance is filled within the second vessel 302.

A biomass containment device (BCD) and methods of measuring microbial growth or enzyme activity in the presence of insoluble substrates using the BCD is described. The BCD is compatible with microbial growth and enzyme assays, is sterilizable, is reusable, and the size can be varied to fit any container.

The invention relates to devices, methods and systems for collecting and solidifying waste material from bioreactor cell cultures in order to facilitate waste disposal. The invention finds particular utility in mammalian cell culture applications. A transverse wall divides the interior of a waste bag into two chambers, the first chamber containing an inlet receiving waste liquid from a bioreactor, the second chamber containing an absorbent material to solidify the liquid into a gel. The transverse wall acts to direct the flow of waste media into the second chamber where it is converted into a gel and further prevents the inlet from being blocked by any gel or particulate materials. Methods and uses regarding this invention are described.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Provided is a cell-culturing apparatus including a culturing unit in which a culturing vessel is placed and an incubator in which a plurality of culturing units are placed in an interior thereof, and that can maintain the interior thereof at an environment that is appropriate for cell culturing, wherein the incubator includes openings that are provided for each of the plurality of culturing units and through which the culturing units can separately be moved into and out of the incubator, and closing means that are provided at the openings and that seals off the internal environment of the incubator from outside when the culturing unit is taken out of the incubator.

This disclosure describes a biochemical reactor with a lower divider support structure. The biochemical reactor may include a tank configured to house immobilized carriers and fluid. The biochemical reactor may include a circulation conduit at least partially disposed within the tank. The circulation conduit may include a circulation outlet opening. The biochemical reactor may include one or more vanes disposed proximate to the circulation outlet opening. The biochemical reactor may include a tank recirculation port disposed proximate to a second end. The biochemical reactor may include a tank inlet configured for feeding fluid into the tank. The biochemical reactor may include a tank outlet configured for drawing fluid from the tank. The tank outlet may be disposed proximate to a first end. The biochemical reactor may include a first divider and a second divider. The second divider may include a support structure including a grating configured to withstand variable loads.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.