The present disclosure provides a system for mimicking the secondary lymphoid organs where suspension cells (e.g., T cells) are expanded; methods el expanding activating, and transfecting the suspension cells in the synthetic, microenvironment, and suspension cells produced by such systems and methods.

A configurable system for repeatably performing processes related to tissue growth in a controlled environment, possibly part of an industrial production line. The system can accommodate various sizes and shapes of culture vessels, and can maintain the cells at a desired temperature in the culture vessels, thus enabling a plug and play system that can produce consistent and repeatable results. The system includes gas management, fluid management, and control of multiple processes simultaneously, and can be automatically operably coupled with a variety of supporting technologies that can enable tissue-related processes. The system can communicate with supporting technologies upstream and downstream on a manufacturing line, enabling fully automated process control, centralized data historization, and centralized control.

Disclosed herein are systems and methods for testing effects of simulated microgravity on muscle structures. The system includes a clinostat and an adapter that is receivable into the clinostat. The adapter includes a housing that defines at least one receptacle. The system further includes an assembly having a substrate and a plurality of fibrils deposited on the substrate. The plurality of fibrils comprise collagen and extend along an axis. The assembly is receivable into a first receptacle of the at least one receptacle. The plurality of fibrils can have myoblasts thereon. Operation of the clinostat simulates microgravity. Test substances can screened using the muscle structures to determine effects on myogenesis.

A cell culture device includes: a base; a rotary table attached to the base; a container holding member attached to the rotary table and configured to hold a cell culture container; and a drive mechanism configured to rotatably drive the rotary table around a first axis and the container holding member around a second axis, wherein a liquid port to which a liquid tube is connected is arranged on an outer peripheral surface of the cell culture container, wherein a first predetermined portion of the liquid tube is positioned on the container holding member by a first positioning member, wherein a second predetermined portion of the liquid tube is positioned on the rotary table by a second positioning member, and wherein the first positioning member is rotatably attached to the container holding member.

A culture vessel configured for culturing biological cells including a body being configured to be rotated about an axis of rotation, the body including one or more helical conduit configured to receive a fluid and extending along an exterior surface of the body, the body defining a reservoir configured to receive the fluid, and the body defining a first aperture and a second aperture each in communication with the helical conduit and the reservoir and configured for passage of the fluid, and the culture vessel further optionally including a support being received within the reservoir of the body, the support including a permeable membrane configured to accommodate biological cells, wherein the helical conduit of the body is configured to facilitate movement of the fluid between the first aperture and the second aperture of the body during rotation of the body.

A tissue culture system, comprising: a support configured to be rotated about an axis of rotation; and one or more culture vessels configured to be mounted to at least a portion of the support, a culture vessel comprising: (i) a chamber configured to receive a biological tissue, and (ii) a conduit in communication with the chamber and configured to facilitate a flow of a composition, wherein the support is configured to cause rotation of the one or more culture vessels during rotation of the support, and wherein the conduit is configured to allow for flow of the composition through the chamber during rotation of the culture vessel. Related methods and tissue treatment compositions are also provided.