An algal growth method includes the steps of providing an algal growth system, the algal growth system including a frame, a first flexible sheet material mounted on a first frame in a first mounted geometry, the first flexible sheet material having a substantially vertical orientation when mounted on the first frame, a second flexible sheet material mounted on a second frame in a second mounted geometry, where the second flexible sheet material has a substantially vertical orientation, a first drive shaft, a second drive shaft, an actuator system, wherein the actuator system is coupled with the first drive shaft and the second drive shaft, a motor, a harvesting mechanism, and a reservoir, and operating the algal growth system, and harvesting algae from the algal growth system.

The present invention relates to bioreactor system and method thereof wherein support matrix (2) comprises at last one central shaft and plurality of peripheral shaft being radially surrounds central shaft. Arrays of discs (11) are mounted along the shaft by defining interspatial vicinities between two successive plates. Thus, discs mounted on peripheral shafts are rotated within the interspatial vicinity of discs of central shaft to ensures sufficient mixing and avoid stagnant fluidic zones which is created when discs are mounted closely apart from each other on shafts. Further, plurality of deflector vanes that are axially provided along the length of the central shaft to redirect substantially co-axial direction fluid flow into interior of culture vessel and more specifically towards the central axis. Thus, bioreactor system provides scalable and disposable bioreactor with efficient mixing and homogeneous conditions and thereby supports high density growth and maintenance of cells and other biological material.

An algal growth system including a first flexible sheet material mounted in a first mounted geometry such that the height is greater than the width of the first mounted geometry, and a second flexible sheet material mounted in a second mounted geometry such that the height is greater than the width of the second mounted geometry, the first flexible sheet and the second flexible sheet material being noncontiguous, a motor, the motor being coupled with an actuator system, where the motor actuates the actuator system, and a reservoir.

Embodiments for loading and expanding particular cell types are described. Embodiments may include the use of hollow fiber membranes with particular characteristic such as hollow fibers with inner diameters that provide mechanical stimulus (e.g., radius of curvature greater than a dimension of a cell). In addition, embodiments may provide for manipulation of flow rates and other features that also provide mechanical stimuli and promote or enhance the growth of particular types of cells.

A culture device supplying an accurate amount of liquid into a culture bag has a culture bag having ports, a first bag holding portion having supporting surfaces supporting the culture bag, a rotation mechanism rotating the first bag holding portion, a liquid supplying mechanism supplying a liquid through a tube communicating with the ports a weight detector detecting the weight of the culture bag and the first bag holding portion, and a control portion, in which the control portion sets a first reference value according to a first detection information output from the weight detector in a first state and carries out a liquid supplying step of stopping the liquid supplying mechanism under the condition where a second detection information output from the weight detector when a liquid is supplied to the culturing bag reaches a first target value obtained by adding the weight of the liquid to be supplied to the first reference value.

A cell culture apparatus including a cell culture device and a movement system for moving the cell culture device. The cell culture device includes at least two reservoirs for holding a liquid cell medium, one or more chambers for culturing of living cells, tissues or living organoids and at least two perfusion channels connecting the reservoirs. The chambers are separated from at least one of the perfusion channels by a semipermeable barrier for selective transport of cell media and/or for selective growth or migration of living cells. The movement system is configured to move the cell culture device with a movement axis in a tilted orientation in order to move the lowest point of a flow loop through all points within the flow path of the flow loop and thereby generate gravity driven circulation of the liquid cell medium around the flow loop.

A method of conducting oxidation in an inflatable bag bioreactor or a batch reactor is provided. The inflatable bag bioreactor or the batch reactor is used as an efficient, economical, and convenient reaction vessel. The inflatable bag bioreactor or batch reactor is rotated or rocked during the reaction to ensure continued exposure of the reaction mixture to the headspace gas in the vessel. The ability of the inflatable bag to expand or contract as the volume of the contents changes helps maintain consistent pressure and avoids the need to replenish the headspace gas.

An algal growth system can include a frame and a flexible sheet material that can have a substantially vertical orientation, where the flexible sheet material can facilitate the growth and attachment of algae, where the flexible sheet material can be supported by the frame.

A fluidic system for producing extracellular vesicles from producer cells, including at least one container, a liquid medium contained by the container and producer cells, characterised in that it also includes microcarriers suspended in the liquid medium, the majority of producer cells being adherent to the surface of the microcarriers, and a liquid medium agitator, the agitator and the dimensions of the container being adapted to control a turbulent flow of the liquid medium in the container.

A bioreactor liquid agitation device may include a rack which may be movably coupled to a frame. Preferably, a motivator which may be configured to motivate the rack in a movement circuit. A rack may comprise one or more sets of rails. Each rail may comprise a channel which may be formed by two opposing retaining walls which may be coupled to and separated by a central wall. A set of two rails, with each rail having its channel facing the other rail, may be configured to support one or more bioreactors by receiving opposing portions of a vessel coupler of each bioreactor in the two channels. An arrester may be coupled to the rack(s), and the arrestor may block the vessel couplers from exiting an end of the channels so that the vessel couplers may only enter and exit the channels from an opposing end of the channels.