An apparatus for substantially horizontally oscillating one or more vessels containing a liquid, a solid, or a mixture thereof that operates to thoroughly disrupt and mix solid and liquid substances in vessels, like test tubes. The apparatus provides superior mixing through oscillation of the vessels both horizontal and vertical directions through the use of one or more springs and rotating mechanical components. The apparatus includes a circular rotating ring having a toothed circumference that rotates horizontally, at least one vessel support having a compartment configured to hold the one or more vessel vessels and a spring attached to the housing and in constant contact with each of the one or more vessel supports.

An apparatus for substantially horizontally oscillating one or more vessels containing a liquid, a solid, or a mixture thereof that operates to thoroughly disrupt and mix solid and liquid substances in vessels, like test tubes. The apparatus provides superior mixing through oscillation of the vessels both horizontal and vertical directions through the use of one or more springs and rotating mechanical components. The apparatus includes a circular rotating ring having a toothed circumference that rotates horizontally, at least one vessel support having a compartment configured to hold the one or more vessel vessels and a spring attached to the housing and in constant contact with each of the one or more vessel supports.

A microalgae photochemistry culturing apparatus includes a photochemistry culturing machine for culturing microalgae by photochemical reactions; a red variation photochemistry culturing machine, which is connected to the photochemistry culturing machine to mix a nitrogen starvation medium filled therein with the microalgae precipitated in the photochemistry culturing machine and allows the nitrogen starvation medium to subject the microalgae to red variation to produce red variation microalgae in which astaxanthin is produced; a floatation separator enriching the red variation microalgae precipitated and emitted from the red variation photochemistry culturing machine through microbubbles fed; and a centrifugal separator removing residual moisture from the red variation microalgae enriched in the floatation separator and collecting the red variation microalgae.

Disclosed is a device for angling a multi-well plate during shaking on a typical low speed laboratory incubator-shaker to facilitate cell growth. An assembly with a holder for a deep-well multi-well plate to attach it to a base has an adjustable-angle joint. The base is attached to a conventional incubator shaker tray via screws or a sticky pad. The adjustable-angle joint allows maintaining the desired angle of the plate, even at full shaking speed.

A compact rotary algae biofilm reactor includes an algae biofilm module and a light guide module. The algae biofilm module includes a plurality of rotary members and a reaction tank. In the rotary member, an algae biofilm growth carrier and a power transmission belt are wound on a driving shaft and a driven shaft. A top end of the rotary member is fixed on a fixing frame while a bottom end thereof is submerged in the reaction tank. The light guide module includes a rotatable lens holder. The rotatable lens holder is fixedly provided with a linear Fresnel lens on an upper end thereof and is provided with a light guide plate on a lower end thereof. One side of the linear Fresnel lens is provided with a step motor configured to control the linear Fresnel lens to rotate with an angle of sunlight.

An automated cell culturing device, which expands, detaches and prepares cells, ready to be implanted in vivo is disclosed. The device is composed by a multi-layered cell culture chamber, a cell preparation chamber, and critical parameters control units which automatically drive the cell culture medium circulation, change and refill. The device according to the invention is characterized in that all the components contacting cells and culture medium constitute a totally disposable cartridge in order to avoid cross-contamination and improve safety. The device is particularly useful for expanding and preparing mesenchymal stem cells for osteoarthritis (OA) therapy, and for other cell based therapies in mammals.

A compact rotary algae biofilm reactor includes an algae biofilm module and a light guide module. The algae biofilm module includes a plurality of rotary members and a reaction tank. In the rotary member, an algae biofilm growth carrier and a power transmission belt are wound on a driving shaft and a driven shaft. A top end of the rotary member is fixed on a fixing frame while a bottom end thereof is submerged in the reaction tank. The light guide module includes a rotatable lens holder. The rotatable lens holder is fixedly provided with a linear Fresnel lens on an upper end thereof and is provided with a light guide plate on a lower end thereof. One side of the linear Fresnel lens is provided with a step motor configured to control the linear Fresnel lens to rotate with an angle of sunlight.

The disclosure relates to bioreactors, for example for biological treatment and, more specifically to bioreactor insert apparatus including biofilms and related methods. The bioreactor insert apparatus provides a means for circulation of reaction medium within the bioreactor, a biofilm support, and biological treatment of an inlet feed to the reactor/insert apparatus. The bioreactor insert apparatus has a high relative surface area for biofilm attachment and is capable of generating complex flow patterns and increasing treatment efficiency/biological conversion activity in a biologically-active reactor. The high surface area structure incorporates multiple biofilm support structures such as meshes at inlet and outlet portions of the structure. The biofilm support structures and biofilms thereon can increase overall reaction rate of the bioreactor and/or perform some solid/liquid separation in the treatment of the wastewater or other influent.

A multi-layer culture vessel operation system capable of easily performing a multi-layer culture vessel handling operation is provided. A multi-layer culture vessel operation system includes: a cart device 20 movable with a multi-layer culture vessel 30 mounted thereon; and an operational device 10 capable of holding and rotating the multi-layer culture vessel 30, wherein the cart device 20 includes: a cart 21 having wheels 22; and a fixing part 23 that is removably mounted on the cart 21, and fixes the multi-layer culture vessel 30 to the cart, wherein the operational device 10 includes: a rotating portion 11 that holds the multi-layer culture vessel 30 and performs a rotation operation of rotating the multi-layer culture vessel 30 around a first rotation axis and/or a second rotation axis; a shaking portion 13 that holds the multi-layer culture vessel 30 and performs a shaking operation of shaking the multi-layer culture vessel 30 in a horizontal direction; and a control portion 15 that causes the shaking portion 13 to perform the shaking operation following the rotation operation by the rotating portion 11, without returning the multi-layer culture vessel 30 to the cart 21.

A method of differentiating induced pluripotent stem cells using a hollow fiber bioreactor includes obtaining induced pluripotent stem cells in the hollow fiber bioreactor and causing a differentiating medium to move through the hollow fiber bioreactor. In certain variations, the obtaining of the induced pluripotent stem cells may include expanding the induced pluripotent stem cell using the hollow fiber bioreactor. In other variations, the obtaining of the induced pluripotent stem cells may include introducing the induced pluripotent stem cells into the hollow fiber bioreactor.