The present invention relates to a bioreactor including at least one reactor vessel in form of a plastic bag, a tray (4) for holding said at least one bag, a rocking device for limited rocking motions of the tray (4) around a rocking axis (2). According to the invention the bioreactor comprises a device (6,7,8,9) for enabling swinging of the tray (4) around a second axis (7) parallel to and distanced from the rocking axis (2).

The insert shelf (1) is characterized in that it has at least one means (3) with which an incubation medium located in an incubation vessel placed on the insert shelf (1) can be set in motion during an incubation treatment or during an incubation process in an incubator (2). For this purpose, the at least one means (3) can be operated electrically and/or preferably moved mechanically.

Embodiments disclosed herein relate to novel apparatuses, methods and systems employing said novel apparatuses, and sensors for use with said apparatuses and systems. In some embodiments, apparatuses and systems described herein are used to culture cells and/or detect changes in pH levels or dissolved oxygen and/or carbon dioxide levels in a cell culture sample resulting from the growth of living cells contained within a reaction vessel such as a flask. In some embodiments, said novel apparatuses may be used in combination with a rocker or shaking device or apparatus. The systems and methods may be used to detect changes in pH and dissolved gas levels in a liquid contained in the reaction vessel due to growth of living cells.

A gas supply device for operation in an incubator allowing treatment of a culture within a shaker flask with gas independently of the composition and humidity of the gas atmosphere within the incubator individually supplies each shaker flask of the incubator with gas using a gas supply clamp detachably connected to the closure cap of each flask. The gas supply clamp has at least two elastic clamping jaws and a web connecting the clamping jaws to one another. The clamping jaws exert a clamping force on the side wall of the closure cap. The clamp is held in place by the elastic clamping jaws. Above a sterile filter in the closure cap, an individual gas atmosphere for treatment of the culture in the shaker flask is produced in a recess of the web above a gas passage in the closure cap.

The invention relates to a separator for retaining and recirculating cells in a continuous-flow or batch-flow type plastic bag or bottle, which can preferably be operated outside of a bioreactor. Additionally, the invention relates to a method for retaining and recirculating cells within or outside a bioreactor. The invention further relates to a method for producing the separator according to the invention.

A cell culture apparatus including cell culture units U and a measurement part (5). The measurement part (5) is provided with an imaging part (51) and is disposed so as to be capable of moving up and down between a back panel part (21) of a casing (2) and a culture container accommodating shelf (32). When the measurement unit (5) reaches a measurement position of each cell culture unit U which is a measurement subject, the culture container accommodating shelf (32) slides toward the measurement unit (5) so that portions to be measured of culture containers U20, U30 in each cell culture unit U come in an imaging range of the imaging part (51).

A culturing device (1) includes a carrier unit having a carrier (2) to which a culture subject adheres, a supply unit (3) that supplies a culture solution to the carrier (2), and a reservoir unit (5) in which the culture solution flowing out from the carrier (2) is stored. The carrier (2) includes a base (21) and a plurality of protrusions (23) which are formed on the surface of the base (21) and of which a relative position of each tip (233) with respect to the base (21) can be changed. The carrier unit includes a swinging mechanism (27) that swing the the carrier (2) and changes the relative positions of the protrusions (23).

Disclosed is an incubator shaker (1) for cell cultivation, which comprises an incubation chamber (10), a shaking table (21) disposed in the incubation chamber (10), and an ozone distributing device (100) for distributing ozone in the incubation chamber (10) including a space underneath the shaking table (21). The shaking table (21) is configured to place a cultivation container thereon during a cultivation process. This incubator shaker (1) can be used for a further disclosed method for decontaminating the incubator shaker (1), including the steps of placing the ozone distributing device (100) into the incubation chamber (10), guiding ozone into the incubation chamber (10), and conveying the ozone inside the incubation chamber (10) including a space underneath the shaking table (21).

A method of manufacturing a composite sensor assembly includes: providing a port including a high surface tension thermoplastic, the port having a hollow tubular portion and a base having a polymer surface; fusing the polymer surface of the port to a wall of a polymeric bioprocess vessel so that the port communicates with an opening in the wall; and retaining a sensor unit within the hollow tubular portion of the port with an adhesive, at least a portion of the sensor unit being polymeric, wherein the port provides the sensor unit access to an interior of the polymeric bioprocess vessel.

The present disclosure discloses an incubator, comprising: a housing defining an incubation chamber, and the incubation chamber is a sealable structure; a shaker located inside the incubation chamber; one or more vent panels, which are each provided with a plurality of vent openings, wherein the incubation chamber is divided into a first area and a second area by the vent panels and the housing, and the shaker is located inside the first area; and a blowing device, for guiding gas flow; wherein, gas in the incubation chamber is driven to circulate between the first area and the second area through cooperation of the vent panels, an inner wall of the housing and the blowing device.