Incubators are disclosed which include an enclosure with an internal chamber configured to support a cell culture plate and provide an environment suitable for maintaining and/or culturing biological cells. The enclosure can include one or more openings configured to allow access to the cell culture plate. The incubators can further include a structure having a plurality of openings configured to be aligned with a corresponding plurality of wells in the cell culture plate, and a sealing element configured to moveably seal the plurality of openings in the structure. The sealing element can comprise a plurality of openings corresponding to at least a subset of the plurality of openings of the structure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the structure. Methods for using the incubators are also provided.

The invention relates to a sterile connector for the sterile transfer of a liquid medium, in particular a biological medium, from a liquid container (2) into a fluid chamber (3, 3), wherein the sterile connector (1) has a first coupling device (5) and a second coupling device (6). It is proposed that the first coupling device (5) has a fluid inlet (7) and a fluid outlet (8) and also a first cannula (9), the latter having an end which is directed away from the fluid inlet (7) and forms the fluid outlet (8), that the second coupling device (6) has a fluid passage (10) which, in an initial state of the sterile connector (1), is covered axially by a first septum (11), that the fluid outlet (8), in the initial state of the sterile connector (1), is arranged in a hermetically sealed region of the first coupling device (5) and the surface (11a) of the first septum (11) facing axially away from the fluid passage (10) is arranged in a hermetically sealed region of the second coupling device (6), and that, in a fluidic connection process starting from the initial state of the sterile connector (1), a fluidic connection between the fluid inlet (7) and the fluid passage (10) can be produced by the fact that the end of the first cannula (9) forming the fluid outlet (8) pierces the first septum (11) of the second coupling device (6).

Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.

Embodiments of the invention relate to a Disposable Bioprocess System including a Single-Use-Bioreactor, a Single-Use-Pump and a single-use micro-organism retention filter. Combined most suitable for cultivation of suspended micro-organisms in a liquid media at high micro-organism concentration in a perfusion mode continuous process for expression of biological material. The inlet port of the liquid Single-Use-Pump connects via a valve to the broth reservoir of the Single-Use-Bioreactor through a liquid conveying port. The outlet port of the liquid pumping Single-Use-Pump connects via a valve to a micro-organism retention filter. And a method for operating said sterile Disposable Bioprocess System in perfusion mode for continuously processing.

Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.

A method and apparatus of aseptic processing and production of cells in a non-sterile enclosure apparatus without chemical biocides is disclosed, by controlling the level of humidity throughout the enclosure to 25% relative humidity (RH) or less, and preferably 20% or 15% or less RH. In addition, the temperature is controlled to 37° C., and consistent gas flow is maintained the enclosure. Colony forming units from microbial contamination detected by environmental monitoring within the enclosure are significantly reduced in this method.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.

Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.

A functional element for a beta container of a transfer system, wherein the functional element is arrangeable on and/or in a beta port opening of a beta port, and the functional element comprises a functional element opening that is smaller than the beta port opening. There is also disclosed a functional element for a beta container of a transfer system, wherein the functional element is arrangeable on and/or in a beta port opening of a beta port, the functional element comprises one or more nutrient medium carrier holders, and each nutrient medium carrier holder is configured to hold a nutrient medium carrier. Furthermore, there is also disclosed a beta container for a transfer system, a transfer system, and a barrier system, such as an isolator system.