The subject invention provides systems, apparatuses and methods for cultivating microorganisms and for producing microbial metabolites on a large scale. Specifically, in certain embodiments, a system comprising three separate, but connected, vessels is provided, wherein a first vessel serves as a feed tank for supplying nutrient medium to a second vessel, said second vessel serving as a submerged fermentation reactor; and wherein a third vessel serves as a collection container into which foam containing microbial growth by-products is transferred from the second vessel.

This disclosure describes hardware for microfluidic chips and an associated support module for facilitating operation of one or more microfluidic chips. The microfluidic chips described herein are designed for supporting multiple different tissue types, including kidney tissue, liver tissue, adipose cells, and so forth. Chip geometry facilities fluid flow through one or more channels of the chip with a particular flow rate. For example, shear forces are reduced where needed to ensure proper flow rate of fluid in the channels. The chamber geometry and the geometry of the channels ensures that a desired amount of oxygen is delivered to sample cells or tissues in a controlled manner.

A Disposable Bioprocess System having a Single-Use-Bioreactor, a Single-Use-Pump and a single-use micro-organism retention filter. Combined most suitable for cultivation of suspended micro-organisms in a liquid media at high micro-organism concentration in a perfusion mode continuous process for expression of biological material. The inlet port of the liquid Single-Use-Pump connects via a valve to the broth reservoir of the Single-Use-Bioreactor through a liquid conveying port. The outlet port of the liquid pumping Single-Use-Pump connects via a valve to a micro-organism retention filter. And a method for operating the sterile Disposable Bioprocess System in perfusion mode for continuously processing.

This cell production device comprises: a cell production plate with a first side and a second side, comprising a cell induction and culture tank configured to perform at least one of induction and culture of cells, and a culture medium reservoir tank configured to store a culture medium to be supplied to the cell induction and culture tank; a warming element that is arranged at or near the first side of the cell production plate and that warms the cell induction and culture tank; and a cooling element that is arranged at or near the second side of the cell production plate and that cools the culture medium reservoir tank.

The present invention relates in a first aspect to a plug flow tubular bioreactor having an integral or multi-part tube adapted for cultivation, and, optionally, infection, viral transduction, or transfection of eukaryotic cells. In a further aspect, the present invention relates to a plug flow tubular bioreactor system comprising the plug flow tubular bioreactor according to the present invention. Further, the present invention relates to a method for preparing virus particles, vectors, cells or other molecules including toxic molecules using the plug flow tubular bioreactor or the system according to the present invention. Finally, the present invention relates to the use of a plug flow tubular bioreactor for the preparation of virus particles, vectors including viral vectors, cells including modified cells or other molecules including toxic molecules.

An observation system includes an observation apparatus including a housing having an arrangement surface for placement of a sample, the sample including a culture medium, and an external illumination unit which is disposed outside the housing and includes at least one light source configured to emit illumination light. At least a part of the arrangement surface is formed of a transparent member having an optically transparent property. The observation apparatus includes an imaging unit which is provided in the housing and includes an image sensor configured to image, via the transparent member, the sample illuminated by the illumination light from the external illumination unit to acquire images of at least three colors.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

The present disclosure provides an integrated and energy-efficient apparatus for the fabrication of completely biodegradable Polyhydroxyalkanoate, PHA, bioplastic products. The apparatus combines a bioreactor fermenter, mixing chambers, and an oven and mold chamber in a single compact design, allowing the entire fabrication process to be handled by a process controller if desired. Plates, cups, masks, packaging materials, and other plastic products can be produced directly from biomass media, with solvents and other agents recycled within the apparatus between batches.

Method for producing bioproducts from streams of organic material, comprising the following steps: (i) the physical-biological pre-treating of the organic stream with water and one or more mechanical steps; (ii) setting the pH value of the mixture between pH 5 and pH 9; iii) adding an inoculum of a natural anaerobic culture that releases organic compounds; iv) a first separation step which splits the mixture into a solid and a liquid fraction, after which an anaerobic fermentation processes the solid fraction, with formation of biogas and digestate; v) an aerobic treatment of the separated liquid fraction with biological conversion of the organic compounds to a protein-rich bioproduct; vi) a second separation step with separation of the formed protein-rich bioproduct; vii) recirculating the liquid phase; viii) drying the formed protein-rich bioproduct, such that in the end a dry, protein-rich bioproduct is obtained as well as the bioproducts biogas and digestate.

A bioreactor sensing system is presented. The bioreactor sensing system comprises a bioreaction vessel. The bioreaction vessel comprises an aperture configured to provide an interface for monitoring contents within the bioreaction vessel. The bioreaction vessel also comprises a port coupled to the bioreaction vessel proximate the aperture. The bioreactor sensing system also comprises a sensing device disposed at least partially within the port such that a sensing element is exposed to the contents. The bioreactor sensing system also comprises an external seal configured to be applied over a portion of the sensing device and a portion of the port.