A deepwater photobioreactor system including a vertical stack extending between an ocean surface and an ocean floor. The vertical stack includes an inlet conduit and an outlet conduit where the inlet conduit is arranged to transport at least seawater and the outlet conduit is arranged to transport at least a biomass. The system includes a first photobioreactor in fluid communication with the inlet conduit and the outlet conduit that is connected to the vertical stack via the inlet and outlet conduits at a first position along the vertical stack below the ocean surface. The first bioreactor is arranged to cultivate the biomass. The system also includes a mooring system arranged to anchor the vertical stack to the ocean floor and arranged to receive the biomass via the outlet conduit and output the biomass to a harvest pipeline.

The present disclosure includes an improved method of making a protein. The steps and conditions were described and carried out with significantly improved protein yield, thereby reducing manufacturing cost.

An inverted conical bioreactor is provided for growing cells or microorganisms. The bioreactor has an internal space and a perforated barrier within the vessel, through which a liquid may flow, where cells or microorganisms cannot pass through the perforated barrier. The perforated barrier divides the internal space of the bioreactor into a first chamber and a second chamber. Cells are grown within the second chamber and can be perfused by re-circulating the liquid, for example a growth medium, through the bioreactor. Various inlet ports and outlet ports allow controlling the parameters of flow of the growth medium.

A controller of a sampling apparatus controls a stirrer to operate at a basic speed during a period of circulation of a liquid in a container through a circulation flow path. The stirrer thus stirs the liquid in the container. The controller controls a flow path switching unit to switch a flow path after lapse of a given time period since lowering in operation speed of the stirrer from the basic speed. The liquid circulated in the circulation flow path thus flows out to a branch flow path.

The present disclosure relates to systems, devices, and methods for controlling fluid flow within a cell processing system. In an embodiment, the present disclosure relates to a device having a rotor comprising one or more rollers configured to compress a first fluid conduit, a first lever arm defining a proximal portion and a distal portion, where the first lever arm comprises a first hinge at the proximal portion, and a second hinge in between the proximal portion and the distal portion, a spring coupled to the distal portion, and a second lever arm, where the first and second lever arms are aligned with the rotor and are configured to receive the first fluid conduit.

A bioreactor for the production of organoids includes at least two reactor modules for cultivation of organoids, for example allowing parallel cultivation of organoids. A method for cell culturing using the bioreactor, more specifically a method for the expansion and differentiation of organoids using the bioreactor.

A pneumatic bioreactor includes a vessel containing a fluid to be mixed and at least one mixing device driven by gas pressure. A first embodiment includes a floating impeller that rises and falls in the fluid as gas bubbles carry it upward to the surface where the gas is then vented, permitting the impeller to sink in the fluid. The floating impeller may be tethered to a second impeller with a flexible member and pulley. The mixing speed is controlled with electromagnets in the vessel acting upon magnetic material in the impeller or its guides. In another embodiment, floating pistons mix the fluid, pushing it through a mixing plate with one or more apertures. In a third embodiment, the mixing device is a rotating drum with bubble-catching blades and rotating mixing plates with apertures. The top of the vessel for these mixers may include a closed top and sterile filters.

A deepwater photobioreactor system including a vertical stack extending between an ocean surface and an ocean floor. The vertical stack includes an inlet conduit and an outlet conduit where the inlet conduit is arranged to transport at least seawater and the outlet conduit is arranged to transport at least a biomass. The system includes a photobioreactor in fluid communication with the inlet conduit and the outlet conduit that is connected to the vertical stack via the inlet and outlet conduits at a first position along the vertical stack below the ocean surface. The first bioreactor is arranged to cultivate the biomass and is positioned below the photic zone of the ocean. The system also includes a mooring system connected to the vertical stack and to the ocean floor and arranged to receive the biomass via the outlet conduit and output the biomass to a harvest pipeline.

Bioreactor systems can include a first frame and a second frame, a well plate, a motor plate, a motor, and a controller. The first frame may define a well plate inset and standoff insets for a first set of metal standoffs. The second frame may define a plurality of mounts and a plurality of insets for a second set of metal standoffs. A gear may be positioned in each of the plurality of mounts. A paddle may be coupled to each of the gears. The well plate can be positioned within the well plate inset. The motor plate can be supported by and connected to the first set of metal standoffs and the second set of metal standoffs. The motor can be mounted on the motor plate and operatively connected to one of the plurality of gears.

The embodiments disclose a portable and compact bioreactor for controlled cultivation of mycelium spores, including a bioreactor cartridge with an injection port configured to transfer predetermined nutrients and mycelium spores into the bioreactor cartridge for cultivation of the mycelium spores, a pressure relief valve, a heating element, an air pump, an agitator and a cloud control system configured to analyze empirical data related to cultivation of the mycelium spores to determine the predetermined speed, the predetermined parameters and the predetermined nutrients to produce specific harvest results and to make future automatic adjustments to the predetermined speed, the predetermined parameters and the predetermined nutrients based on the specific harvest results and a bioreactor cloud data app configured to remotely monitor settings of the bioreactor, to allow the user to adjust the settings and to receive recommended settings based on the analysis of the cloud control system.