Patent classifications
C12N15/01
Bacterial genes and isolates for conferring insect resistance
Provided are biologically pure bacterial isolates characterized by a genome structure at least 90% similar to a genome structure of a bacterial species selected from the group consisting of: Streptomyces sp. E128 having an NRRL Accession No. B-67462, Bacillus amyloliquefaciens A190 having an NRRL Accession No. B-67464, Bacillus subtilis P243 having an NRRL Accession No. B-67459, Bacillus thuringiensis M979 having an NRRL Accession No. B-67457, Massilia aurea P63 having an NRRL Accession No. B-67461, Rhodococcus sp. G706, Stenotrophomonas maltophilia E132 having an NRRL Accession No. B-67460, Streptomyces aurantiacus A918, Streptomyces badius O180, Streptomyces mirabilis B670 having an NRRL Accession No. B67463, Streptomyces scopuliridis F427 having an NRRL Accession No. B-67458, and Streptomyces sp. L219. Also provided are whole cell broth or lysates thereof, and polynucleotide, polypeptides and constructs expressing same, compositions-of-matter comprising same and methods using same for killing or inhibiting the development of insects.
Bacterial genes and isolates for conferring insect resistance
Provided are biologically pure bacterial isolates characterized by a genome structure at least 90% similar to a genome structure of a bacterial species selected from the group consisting of: Streptomyces sp. E128 having an NRRL Accession No. B-67462, Bacillus amyloliquefaciens A190 having an NRRL Accession No. B-67464, Bacillus subtilis P243 having an NRRL Accession No. B-67459, Bacillus thuringiensis M979 having an NRRL Accession No. B-67457, Massilia aurea P63 having an NRRL Accession No. B-67461, Rhodococcus sp. G706, Stenotrophomonas maltophilia E132 having an NRRL Accession No. B-67460, Streptomyces aurantiacus A918, Streptomyces badius O180, Streptomyces mirabilis B670 having an NRRL Accession No. B67463, Streptomyces scopuliridis F427 having an NRRL Accession No. B-67458, and Streptomyces sp. L219. Also provided are whole cell broth or lysates thereof, and polynucleotide, polypeptides and constructs expressing same, compositions-of-matter comprising same and methods using same for killing or inhibiting the development of insects.
PLANT WITH REDUCED WOUND-INDUCED SURFACE DISCOLORATION PHENOTYPE
This disclosure relates to plants having a modified F5H gene which confers the trait of reduced wound-induced surface discoloration. The disclosure further relates to all progeny, seed, and plant parts of said plant. Furthermore, the disclosure relates to a propagation material suitable for producing said plants, and to methods for selecting and producing said plants.
PLANT WITH REDUCED WOUND-INDUCED SURFACE DISCOLORATION PHENOTYPE
This disclosure relates to plants having a modified F5H gene which confers the trait of reduced wound-induced surface discoloration. The disclosure further relates to all progeny, seed, and plant parts of said plant. Furthermore, the disclosure relates to a propagation material suitable for producing said plants, and to methods for selecting and producing said plants.
USE OF ST GAL(+) BACTERIA FOR PRODUCING A FERMENTED MILK PRODUCT WITH A RELATIVELY HIGH STABLE PH
A method for producing a fermented milk product (e.g. a yogurt) with a relatively high stable pH value at the end of the fermentation comprising inoculating milk with Streptococcus thermophilus (ST) Gal(+) bacteria.
USE OF ST GAL(+) BACTERIA FOR PRODUCING A FERMENTED MILK PRODUCT WITH A RELATIVELY HIGH STABLE PH
A method for producing a fermented milk product (e.g. a yogurt) with a relatively high stable pH value at the end of the fermentation comprising inoculating milk with Streptococcus thermophilus (ST) Gal(+) bacteria.
APPLICATION OF MAL33 GENE DELETION IN IMPROVING TOLERANCE OF SACCHAROMYCES CEREVISIAE TO INHIBITORS IN THE LIGNOCELLULOSE HYDROLYZATES
An application of MAL33 gene deletion in improving the tolerance of Saccharomyces cerevisiae to inhibitors in a lignocellulose hydrolyzate is provided. The tolerance of the present MAL33 gene-deleted Saccharomyces cerevisiae strain to acetic acid is greatly improved, and the tolerance of the Saccharomyces cerevisiae strain to other typical inhibitors and H.sub.2O.sub.2 in the lignocellulose hydrolyzate is also improved. The lag period of the Saccharomyces cerevisiae strain in a glucose and xylose medium (YPDX) with 3.5 g/L acetic acid is shortened by 24 h. The fermentation period of the Saccharomyces cerevisiae strain to produce ethanol through co-utilization of glucose and xylose is shortened by 20 h. The growth of the Saccharomyces cerevisiae strain in a glucose and xylose medium (YPDX) with a mixed inhibitor and the ethanol production of the Saccharomyces cerevisiae strain through the co-fermentation of glucose and xylose are superior to those of a control strain.
APPLICATION OF MAL33 GENE DELETION IN IMPROVING TOLERANCE OF SACCHAROMYCES CEREVISIAE TO INHIBITORS IN THE LIGNOCELLULOSE HYDROLYZATES
An application of MAL33 gene deletion in improving the tolerance of Saccharomyces cerevisiae to inhibitors in a lignocellulose hydrolyzate is provided. The tolerance of the present MAL33 gene-deleted Saccharomyces cerevisiae strain to acetic acid is greatly improved, and the tolerance of the Saccharomyces cerevisiae strain to other typical inhibitors and H.sub.2O.sub.2 in the lignocellulose hydrolyzate is also improved. The lag period of the Saccharomyces cerevisiae strain in a glucose and xylose medium (YPDX) with 3.5 g/L acetic acid is shortened by 24 h. The fermentation period of the Saccharomyces cerevisiae strain to produce ethanol through co-utilization of glucose and xylose is shortened by 20 h. The growth of the Saccharomyces cerevisiae strain in a glucose and xylose medium (YPDX) with a mixed inhibitor and the ethanol production of the Saccharomyces cerevisiae strain through the co-fermentation of glucose and xylose are superior to those of a control strain.
Mutant algal strain and methods thereof
A mutant algal strain showing upregulation of mRNA transcripts encoding urea carboxylase, Δ-15-ω3-desaturase and downregulation of mRNA transcripts of gene encoding triacylglycerol lipase is provided herein. The mutant algal strain of the present disclosure is tolerant to low temperature and thus can be grown over a wide temperature range. The strain shows enhanced biomass and fatty acid production and enhanced growth rate and nitrogen metabolism over a wide temperature range of about 10° C. to about 37° C., wherein the enhancement is in comparison to the wild type algal strain. A method of obtaining the mutant algal strain and a method of producing industrially relevant products such as fatty acids from the mutant algal strain also are provided herein.
<i>Saccharomyces cerevisiae </i>kwon P-1, 2, 3 which produce aldehyde dehydrogenase and glutathione
A yeast strain producing glutathione (GSH) and aldehyde dehydrogenase, and more specifically, the yeast strains Saccharomyces cerevisiae Kwon P-1 KCTC13925BP, Saccharomyces cerevisiae Kwon P-2 KCTC14122BP, and Saccharomyces cerevisiae Kwon P-3 KCTC14123BP, which produce both glutathione and aldehyde dehydrogenase.