The present invention relates to a cell reprogramming method using a physical stimulation-mediated environmental influx, and more specifically, by subjecting differentiated or non-differentiated cells to physical stimulation which can promote an environmental influx, such as ultrasonic waves, laser or heat shock, without the introduction of a reprogramming-inducing factor or a chemical substance to the differentiated cells, the cells can be reprogrammed with just the imposition of an external environmental influx into pluripotent cells or arbitrary differentiated cells having a different expression type from the differentiated or non-differentiated cells, and as such an inducement has a simple and effective production process, the possibility of an autogenic cell therapy can be made greater.

A method for isolating and/or culturing cambium stem cells of panax ginseng is disclosed. The method comprises a step of treating tissues containing panax ginseng cambium with a compound of formula I. The present application further relates to cambium stem cells of panax ginseng obtained according to the method and use of such cambium stem cells in preparing a product for a suspension culture of panax ginseng. The method for isolating and/or culturing cambium stem cells of panax ginseng according to the present application can effectively separate the cambium stem cells of the panax ginseng which have unlimited cytodieresis ability and strong anti-adversity ability, can provide a basis for ultra-large volume liquid culture, which can significantly reduce production costs.

Provided herein are systems and methods for molecule delivery into cells. The method may include steps of forming a solution comprising an amount of microbubbles, an amount of soluble molecules, and one or more cells, flowing the solution through a microfluidic device, and sonicating the solution for a period of time sufficient to form pores in the one or more cells. In particular, such methods may be used for preservation of cells through delivery of a preservation agent.

The present invention is directed to indirect ultrasonic cavitation-derived perivascular cells, to methods of use of a perivascular cell composition, to a method of processing a tissue and to an apparatus for the processing of a tissue. The methods include the mechanic indirect ultrasonication of a cellular non-structural tissue, and produce a perivascular fraction which includes perivascular cells. The methods of use are directed to the treatment of a variety of diseases and disorders and to the improvement of a tissue in a subject. The apparatus is provided for the processing of cellular non-structural tissue.

The present disclosure provides a reprosome that can induce reprogramming of a cell, in which the reprosome is characterized by including RNA of a gene involved in chromatin remodeling, in which the gene includes a kinase gene on a mitogen-activated protein kinase (MAPK) signal transduction system, and a gene having histone modification activity. The reprosome may be obtained from a stem cell difficult to process and/or from a readily obtainable somatic cell via a simple process including ultrasonic treatment. A reprogramming of one kind of a cell into another kind of a cell with a desired function can be achieved at a high efficiency in a short time via a simple treatment including a co-culturing between the reprosome and the cell. The cell thus obtained has the desired function without introduction of a chemical or foreign transcription factor into a genome and thus is more suitable for cell replacement therapies. Further, the present disclosure provides a composition including the reprosome and a method for regenerating a tissue by treating a body site with the composition to promote reprogramming of a cell present in the treated body site to a target cell having a desired function.

The present invention relates to a cell reprogramming method using a physical stimulation-mediated environmental influx, and more specifically, by subjecting differentiated or non-differentiated cells to physical stimulation which can promote an environmental influx, such as ultrasonic waves, laser or heat shock, without the introduction of a reprogramming-inducing factor or a chemical substance to the differentiated cells, the cells can be reprogrammed with just the imposition of an external environmental influx into pluripotent cells or arbitrary differentiated cells having a different expression type from the differentiated or non-differentiated cells, and as such an inducement has a simple and effective production process, the possibility of an autogenic cell therapy can be made greater.

Provided herein are methods of intracellular delivery of a substance to one or more cells. The methods include providing a substrate defining a micro-channel in fluid communication with a first chamber and optionally in fluid communication with a second chamber, the micro-channel having a hydraulic diameter that is less than a hydraulic diameter of the first and second chambers; and driving a cell suspension through the micro-channel, thereby: i) causing the one or more cells to be stretched along a direction of flow and ii) inducing a formation of one or more temporary pores in a membrane of the one or more cells, wherein the cell suspension comprises the one or more cells, a polymer, and the substance. Also provided are systems for the intracellular delivery of a substance to one or more cells.

In an aspect of the present disclosure there is provided a method for reversibly stimulating neuronal cells, said method comprising activating mechanosensitive ion channels of neuronal cells expressing said channels by exposing said cells to an ultrasound stimulus.

A method for loading immunocompetent cells with nanoparticles and/or a cytotoxic substance is for use in the theranostic treatment of patient-specific genetically modified cells. The method includes the steps: (a) providing immunocompetent cells; and (b) loading the immunocompetent cells with the nanoparticles and/or the cytotoxic substance, wherein the nanoparticles and/or the cytotoxic substance are nuclides for radiotherapy. The loading step includes (i) applying the nanoparticles and/or the cytotoxic substance to the immunocompetent cells or mixing the nanoparticles and/or the cytotoxic substance with the immunocompetent cells in a liquid carrier; and (ii) poration of the immunocompetent cells in order to increase the permeability of the cell membranes of the immunocompetent cells for the nanoparticles and/or the cytotoxic substance and thereby loading the immunocompetent cells with the nanoparticles and/or the cytotoxic substance.

Microparticles and nanoparticles made of various materials that are used in various configurations are disclosed. Such particles can also contain various types of materials as payloads to be used in the separation, segregation, differentiation, modification or filtration of a system or a host anatomy. The microparticles and nanoparticles are utilized in conjunction with an acoustic standing wave or an acoustic traveling wave in various processes.