Systems and methods for manufacturing maltodextrin and protein nutritional products from rice are disclosed. Some embodiments include: milling hydrated rice, digesting with an α-amylase enzyme to form a mixture of maltodextrin and protein, and separating the protein and maltodextrin from one another.

An isoamylase having improved heat resistance and an industrial method for producing maltose from starch.

The isoamylase is an isoamylase consisting of the amino acid sequence represented by SEQ ID NO: 1 or an isoamylase resulting from deletion, substitution, or insertion of one to several amino acid residues in the amino acid sequence represented by SEQ ID NO: 1, wherein at least valine at amino acid number 515 and methionine at amino acid number 570 are mutated to other amino acids.

The present invention relates to a method for producing trehalose, comprising the steps of mixing and reacting, in any order, (i) at least one alpha-phosphorylase capable of catalyzing the production of alpha-D-glucose 1-phosphate intermediate from a saccharide raw material, and from at least one phosphorus source; (ii) at least one trehalose phosphorylase capable of catalyzing the production of trehalose from an alpha-D-glucose 1-phosphate intermediate and a glucose substrate, wherein the trehalose phosphorylase is a trehalose phosphorylase variant with an amino acid sequence which differs from the amino acid sequence of a wild type trehalose phosphorylase in at least one amino acid position, (iii) at least one saccharide raw material which produces an alpha-D-glucose 1-phosphate intermediate and a co-product by catalytic action of the alpha-phosphorylase; and (iv) at least one phosphorus source selected from the group consisting of a phosphoric acids and an inorganic salt thereof.

The present invention relates to a method for producing trehalose, comprising the steps of mixing and reacting, in any order, (i) at least one alpha-phosphorylase capable of catalyzing the production of alpha-D-glucose 1-phosphate intermediate from a saccharide raw material, and from at least one phosphorus source; (ii) at least one trehalose phosphorylase capable of catalyzing the production of trehalose from an alpha-D-glucose 1-phosphate intermediate and a glucose substrate, wherein the trehalose phosphorylase is a trehalose phosphorylase variant with an amino acid sequence which differs from the amino acid sequence of a wild type trehalose phosphorylase in at least one amino acid position, (iii) at least one saccharide raw material which produces an alpha-D-glucose 1-phosphate intermediate and a co-product by catalytic action of the alpha-phosphorylase; and (iv) at least one phosphorus source selected from the group consisting of a phosphoric acids and an inorganic salt thereof.

Disclosed is a method for improving the productivity of 2′-fucosyllactose (2′-FL) through enzymatic treatment. Lactose used as a substrate in the stationary phase during culture is degraded by treatment with a small amount of enzyme, the resulting glucose is consumed to produce guanosine diphosphate-L-fucose as a precursor of 2′-fucosyllactose, and the use of lactose left after culture can be maximally utilized for the production of 2′-fucosyllactose. As a result, it is possible to increase the productivity of 2′-fucosyllactose in an economically efficient manner because additional glucose is not required while minimizing by-products.

The present invention relates to a strain deposited with the CNCM (Collection Nationale de Culture de Microorganismes, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France), under number CNCM I-5499. The present invention also relates to the in vitro use of strains for producing oligosaccharides and/or in a process for producing oligosaccharides.

The present invention finds an application, in particular in the bioproduction field, for example the production of compounds, for example of bio-compounds.

The present invention relates to a strain deposited with the CNCM (Collection Nationale de Culture de Microorganismes, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France), under number CNCM I-5499. The present invention also relates to the in vitro use of strains for producing oligosaccharides and/or in a process for producing oligosaccharides.

The present invention finds an application, in particular in the bioproduction field, for example the production of compounds, for example of bio-compounds.

The present invention relates to a Myceliophthora thermophila host cell which expresses a recombinant enzymes from Fusarium oxysporum with beta-xylosidase activity. The invention also refers to an enzymatic composition comprising the host cell of the invention and/or the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention. The invention further relates to the use of the host cell of the invention, the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention or the composition of the invention for the degradation of biomass and to a method of producing bioproducts, preferably bioethanol, which comprises the use of the host cell of the invention, the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention or the composition of the invention.

The present invention relates to a Myceliophthora thermophila host cell which expresses a recombinant enzymes from Fusarium oxysporum with beta-xylosidase activity. The invention also refers to an enzymatic composition comprising the host cell of the invention and/or the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention. The invention further relates to the use of the host cell of the invention, the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention or the composition of the invention for the degradation of biomass and to a method of producing bioproducts, preferably bioethanol, which comprises the use of the host cell of the invention, the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention or the composition of the invention.

A method of generating a saccharide, especially lactulose or lactosucrose, in the presence of a transgalactosylase enzyme, is described herein.