A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example.

A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example.

The invention provides variants of the Aspergillus japonicus β-fructofuranosidase enzyme which have been modified so as to improve synthesis of inulin-type fructooligosaccharides (FOS) from sucrose. One or more substitutions may be made to the parent β-fructofuranosidase polypeptide at amino acid positions 121, 159, 302 and/or 471 of the mature peptide (SEQ ID NO: 3), corresponding to crystal positions 140, 178, 321 and 490. A method of synthesising FOS using the variants is also claimed.

The invention provides variants of the Aspergillus japonicus β-fructofuranosidase enzyme which have been modified so as to improve synthesis of inulin-type fructooligosaccharides (FOS) from sucrose. One or more substitutions may be made to the parent β-fructofuranosidase polypeptide at amino acid positions 121, 159, 302 and/or 471 of the mature peptide (SEQ ID NO: 3), corresponding to crystal positions 140, 178, 321 and 490. A method of synthesising FOS using the variants is also claimed.

The present invention relates to a method for obtaining a high titer of enzyme mixture comprising cellulases, hemicellulases and β-glucosidases in reutilization of waste water generated during hot water extraction of lignocellulosic biomass or biorefinery waste water using Penicillium funiculosum MRJ-16 mutant strain. The cellulose or lignocellulosic biomass used in the fermentation process is selected from the group consisting of rice straw, wheat straw, corn stover, cotton stalk or a combination thereof. The enzyme mixture obtained by the present process is used for the saccharification of acid pretreated lignocellulosic biomass.

The present invention relates to a method of making glucose syrup from liquefied starch, and to compositions useful therein.

The present invention relates to a method of making glucose syrup from liquefied starch, and to compositions useful therein.

Processes for grinding corn, ground corn products, and processes for making ethanol from the ground corn products. In some examples, a process for making ethanol can include introducing a plurality of corn pieces into a mill. The process can also include milling the corn pieces in the mill to produce a ground corn product. Greater than 25 wt % of the ground corn product can have a particle size of greater than 105 μm, as measured according to AOAC 965.22-1966. Greater than 80 wt % of the ground corn product can have a particle size of 425 μm or less, as measured according to AOAC 965.22-1966. The process can also include processing the ground corn product to produce a fermentation mash that can include ethanol and separating at least a portion of the ethanol from the fermentation mash to produce a stillage.

Processes for grinding corn, ground corn products, and processes for making ethanol from the ground corn products. In some examples, a process for making ethanol can include introducing a plurality of corn pieces into a mill. The process can also include milling the corn pieces in the mill to produce a ground corn product. Greater than 25 wt % of the ground corn product can have a particle size of greater than 105 μm, as measured according to AOAC 965.22-1966. Greater than 80 wt % of the ground corn product can have a particle size of 425 μm or less, as measured according to AOAC 965.22-1966. The process can also include processing the ground corn product to produce a fermentation mash that can include ethanol and separating at least a portion of the ethanol from the fermentation mash to produce a stillage.

Oligosaccharides from bovine milk, whey and dairy products, and methods of producing bovine milk oligosaccharides are provided.