Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids.

Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids.

A thermal cycling method and associated device is described. The method is for carrying out a polymerase chain reaction (PCR) process to amplify deoxyribonucleic acid (DNA), and the method includes: pre-heating a series of blocks to respective temperatures that correspond to different respective heating stages in a PCR process, in which each block of the series of blocks defines a respective heat transfer surface, in which the series of blocks define a sequence of positions along a path, with each position defined by a respective heat transfer surface of a respective block; and moving a PCR reaction vessel, which contains deoxyribonucleic acid (DNA) and PCR reagents, along the path into and out of each respective position in the sequence of positions according to a schedule, in which, at each respective position the PCR reaction vessel is in thermal contact with the respective heat transfer surface to equilibrate a temperature of the PCR reaction vessel to a target temperature that corresponds to a respective heating stage in the PCR process.

A thermal cycling method and associated device is described. The method is for carrying out a polymerase chain reaction (PCR) process to amplify deoxyribonucleic acid (DNA), and the method includes: pre-heating a series of blocks to respective temperatures that correspond to different respective heating stages in a PCR process, in which each block of the series of blocks defines a respective heat transfer surface, in which the series of blocks define a sequence of positions along a path, with each position defined by a respective heat transfer surface of a respective block; and moving a PCR reaction vessel, which contains deoxyribonucleic acid (DNA) and PCR reagents, along the path into and out of each respective position in the sequence of positions according to a schedule, in which, at each respective position the PCR reaction vessel is in thermal contact with the respective heat transfer surface to equilibrate a temperature of the PCR reaction vessel to a target temperature that corresponds to a respective heating stage in the PCR process.

The present disclosure provides systems, devices, and methods for purifying microorganism nucleic acid from a host sample, such as a whole blood sample from a human. In certain embodiments, devices and systems with multiple filters are employed and provide for the selective removal of blood cells and host nucleic acids from a sample in order to enrich for microorganism nucleic acid.

The present disclosure provides systems, devices, and methods for purifying microorganism nucleic acid from a host sample, such as a whole blood sample from a human. In certain embodiments, devices and systems with multiple filters are employed and provide for the selective removal of blood cells and host nucleic acids from a sample in order to enrich for microorganism nucleic acid.

A preparation method for an aerolysin nanopore in this disclosure comprises the following steps; (1) pretreatment of an aerolysin; (2) preparation of a lipid bilayer membrane by pulling process; (3) forming of the aerolysin nanopore: the aerolysin nanopore is obtained at a current of 505 pA. The aerolysin nanopore prepared in the invention is structurally stable and has a high resolution with the whole internal cavity carried with a positive charge, can be used for detection without modification and is easily operated. Further, the aerolysin nanopore can be applied in DNA sequencing, DNA damage and Micro-RNA detection.

A preparation method for an aerolysin nanopore in this disclosure comprises the following steps; (1) pretreatment of an aerolysin; (2) preparation of a lipid bilayer membrane by pulling process; (3) forming of the aerolysin nanopore: the aerolysin nanopore is obtained at a current of 505 pA. The aerolysin nanopore prepared in the invention is structurally stable and has a high resolution with the whole internal cavity carried with a positive charge, can be used for detection without modification and is easily operated. Further, the aerolysin nanopore can be applied in DNA sequencing, DNA damage and Micro-RNA detection.

A system and methods for detection of a nucleic acid including forming a plurality of nucleic acid detection complexes are described, each of the complexes including a nucleic acid analyte, a detection agent and a functionalized probe. The method further including binding the nucleic acid detection complexes to a plurality of functionalized particles in a fluid sample and separating the functionalized particles having the nucleic acid detection complexes bound thereto from the fluid sample using a density media. The nucleic acid analyte is detected by detecting the detection agent.

A system and methods for detection of a nucleic acid including forming a plurality of nucleic acid detection complexes are described, each of the complexes including a nucleic acid analyte, a detection agent and a functionalized probe. The method further including binding the nucleic acid detection complexes to a plurality of functionalized particles in a fluid sample and separating the functionalized particles having the nucleic acid detection complexes bound thereto from the fluid sample using a density media. The nucleic acid analyte is detected by detecting the detection agent.